Article
Microbiology
Tan-Trung Nguyen, Tran T. T. Xuan, To H. Ngoc, Le T. My Duyen, Tu Q. Vinh, Pham D. T. My, Hoang A. Hoang, Le P. Nga
Summary: This study reveals the existence of two novel bacteriophages infecting Edwardsiella ictaluri, vB_EiM_PVN06 and vB_EiA_PVN09, originating from the Mekong Delta in Vietnam. These bacteriophages exhibit distinct biological properties and show potential for phage therapy in the control of ESC disease.
Article
Fisheries
Jillian K. Malecki, Luke A. Roy, Cova R. Arias, Triet Nhat Truong, Terrill R. Hanson, Miles D. Lange, Craig A. Shoemaker, Benjamin H. Beck
Summary: The study investigated the efficacy of the 17-23 vaccine in Channel Catfish fingerlings compared to nonvaccinated fingerlings, showing potential economic benefits to producers. Further validation is needed under both research and commercial settings.
NORTH AMERICAN JOURNAL OF AQUACULTURE
(2021)
Article
Fisheries
Nermeen Y. Abass, Rhoda Mae C. Simora, Jinhai Wang, Shangjia Li, De Xing, Michael Coogan, Andrew Johnson, David Creamer, Xu Wang, Rex A. Dunham
Summary: This study demonstrates that genetic enhancement of the channel catfish's resistance to Edwardsiella ictaluri can be achieved through cecropin transgenesis. The transgenic fish exhibited enhanced disease resistance and improved survival time when challenged with the bacteria. The growth rates of transgenic and non-transgenic fish were not different, indicating that the transgene did not affect the overall growth of the fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Nguyen Van Sang, Tran Thi Phuong Dung, Vo Hong Phuong, Nguyen Hong Nguyen, Nguyen Huu Thinh
Summary: The present study analyzed the immune responses of resistant (RF) and susceptible families (SF) of striped catfish (Pangasianodon hypophthalmus) to Edwardsiella ictaluri, the causative agent of bacillary necrosis of Pangasius. The RF group showed a more effective non-specific immune response compared to the SF group. White blood cell counts increased significantly in the RF group, starting from 48 hours post infection (hpi), while the increase was slower in the SF group, mainly after 264 hpi. Monocyte and neutrophil development was faster in the RF families, and the number of melano-macrophage centers was higher in the RF group at 24-48 hpi. The RF group also exhibited a higher specific immune response (antibody titer) compared to the SF group. The study identified the critical period of 24-48 hpi for differentiating disease resistant individuals, with an Area Under the Curve (AUC) value of 0.95.
Article
Immunology
Xu Zhou, Gui-Rong Zhang, Wei Ji, Ze-Chao Shi, Xu-Fa Ma, Zun-Lan Luo, Kai-Jian Wei
Summary: The study revealed that the immune response of yellow catfish against E. ictaluri infection is mainly manifested in the spleen and liver, with key gene expression peaking at 72 hours post-infection. Cytokines were continuously transcribed in the spleen, while the liver mainly secreted components of the complement system, and metabolic regulation was activated from 12 to 120 hours post-infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Doan Thi Nhinh, Dang Thi Hoa, Nguyen Thi Huong Giang, Kim Van Van, Lua Thi Dang, Mags Crumlish, Ha Thanh Dong, Truong Dinh Hoai
Summary: Widespread distribution of a highly pathogenic Edwardsiella ictaluri strain in farmed tilapia in northern Vietnam has caused a disease outbreak with high fish mortality. The co-infection of E. ictaluri and Flavobacterium oreochromis in tilapia fish exhibited clinical signs of both edwardsiellosis and columnaris diseases, suggesting a synergistic interaction between the two pathogens. Efficient methods to control both pathogens are needed.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Ahmed Abdelmoniem Mousa, Reshma Ramachandran, Ozan Ozdemir, Attila Karsi, Hossam Abdelhamed
Summary: The study showed that dietary trans-cinnamaldehyde (TC) can increase the activity of superoxide dismutase (SOD) and decrease the concentration of malondialdehyde (MDA) in hepatic tissue of channel catfish. Additionally, after infection with Edwardsiella ictaluri, catfish fed with TC demonstrated significantly increased antioxidant defense responses on days 1, 6, and 14 post-infection. However, there were no significant differences in the activities/concentrations of catalase (CAT), reduced glutathione (GSH), nitric oxide (NO), and MDA between the TC and control groups.
Article
Biochemistry & Molecular Biology
Shen-Li You, Xin-Xin Jiang, Gui-Rong Zhang, Wei Ji, Xu-Fa Ma, Xu Zhou, Kai-Jian Wei
Summary: This study cloned and identified the open reading frame sequences of TRAF genes in yellow catfish and found that these genes play important roles in immune response and may be involved in the immune response against bacterial infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Haoxuan Li, Liqiang Zhang, Yunzhen Yu, Taoshan Ai, Yongan Zhang, Jianguo Su
Summary: Yellow catfish is an important freshwater cultured fish in China, but it is susceptible to enteric septicemia and red-head disease caused by Edwardsiella ictaluri, resulting in significant economic losses in the farming industry. This study developed rapid and accurate detection methods, including RPA, real-time RPA, and RPA-LFD, targeting a specific gene sequence in E. ictaluri. These methods demonstrated high sensitivity and specificity, and showed great potential for diagnosing E. ictaluri disease in fish farms.
Article
Infectious Diseases
Ning Xu, Miao Li, Xiaohui Ai, Zhoumeng Lin
Summary: The study found that administering DC at a dose of 20 mg/kg every 96 hours is a preferable regimen in yellow catfish, demonstrating good pharmacokinetic-pharmacodynamic effects against Edwardsiella ictaluri.
Article
Microbiology
Allison L. Wise, Benjamin R. LaFrentz, Anita M. Kelly, Mark R. Liles, Matt J. Griffin, Benjamin H. Beck, Timothy J. Bruce
Summary: A preliminary assessment was conducted to study the bacterial coinfection of Edwardsiella ictaluri and Flavobacterium covae in juvenile channel catfish. The coinfection challenges showed similar cumulative percent mortality (CPM) to the single dose E. ictaluri challenge, but the onset of peak mortality was delayed in fish exposed to F. covae first. Additionally, catfish exposed to E. ictaluri displayed increased serum lysozyme activity and higher expression of pro-inflammatory cytokines.
Article
Fisheries
Bingchao Wang, Fangzheng Zhu, Zechao Shi, Zhenyu Huang, Ruhan Sun, Qingchao Wang, Ouyang Gang, Wei Ji
Summary: In this study, the mhc IIA and mhc IIB genes of yellow catfish Pelteobagrus fulvidraco were cloned and characterized. Similar gene structure and expression patterns were observed for both mhc IIA and mhc IIB, and they play an important role in the anti-infection process.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Adrian Lopez-Porras, Matt J. Griffin, Cynthia Ware, Bradley M. Richardson, Terrence E. Greenway, Thomas Graham Rosser, Suja Aarattuthodiyi, David J. Wise
Summary: Edwardsiella piscicida is a growing problem in catfish aquaculture in the southeastern United States, especially in channel and hybrid catfish. Multiple phyletic groups of E. piscicida have been identified, with no significant differences in virulence between channel and hybrid catfish. A live-attenuated E. ictaluri vaccine has shown cross-protection against one E. piscicida isolate, but its effectiveness against other variants is unknown. Exposure to heterologous E. piscicida isolates improves survival following E. ictaluri challenge, suggesting the presence of shared and conserved antigens.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Jicheng Yang, Yaoyao Lin, Zhaohui Wei, Zhenbing Wu, Qianqian Zhang, Jingwen Hao, Shuyi Wang, Aihua Li
Summary: 16S rRNA gene high-throughput sequencing technology was used to investigate the changes in the gut microbiota of yellow catfish infected by Edwardsiella ictaluri. The microbial composition and diversity of the diseased fish were significantly different from those of healthy fish. Proteobacteria became the dominant phylum in the gut of the diseased fish, while Cetobacterium, Plesiomonas, and Romboutsia disappeared. The gut microbiota of the diseased fish showed reduced anaerobic and Gram-positive bacteria, as well as decreased digestive system, immune system, and metabolic functions.
Article
Fisheries
Uni Purwaningsih, Sukenda Sukenda, Angela M. Lusiastuti, Alimuddin Alimuddin, Widanarni Widanarni, Sri Nuryati
Summary: This study compared the phenotypic, genotypic characteristics, and pathogenicity of Edwardsiella ictaluri from different catfish farms in Indonesia. The results showed variations in growth patterns, enzyme activity, antibiotic resistance, and genetic profiles among the isolates. The protein components of E. ictaluri cells were found to be virulent and played a role in the pathogenesis of E. ictaluri infection in catfish.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
J. C. Garcia, B. R. LaFrentz, G. C. Waldbieser, F. S. Wong, S. F. Chang
JOURNAL OF FISH DISEASES
(2018)
Article
Fisheries
Craig A. Shoemaker, Benjamin R. LaFrentz, Eric Peatman, Benjamin H. Beck
JOURNAL OF FISH DISEASES
(2018)
Article
Microbiology
Benjamin R. LaFrentz, Julio C. Garcia, Geoffrey C. Waldbieser, Jason P. Evenhuis, Thomas P. Loch, Mark R. Liles, Fong S. Wong, Siow F. Chang
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Esteban Soto, Matt J. Griffin, Juan Alberto Morales, Elias Barquero Calvo, Fernanda de Alexandre Sebastiao, Adrian Lopez Porras, Xindy Viquez-Rodriguez, Stephen R. Reichley, Thomas G. Rosser, Cynthia Ware, Barbara A. Byrne, Julio C. Garcia, Benjamin R. LaFrentz, Alvin C. Camush
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Fisheries
Fernanda de Alexandre Sebastiao, Benjamin R. LaFrentz, John P. Shelley, Brittany Stevens, David Marancik, Freeland Dunker, Drury Reavill, Esteban Soto
JOURNAL OF FISH DISEASES
(2019)
Biographical-Item
Fisheries
Stacey A. LaFrentz, Benjamin R. LaFrentz
JOURNAL OF AQUATIC ANIMAL HEALTH
(2019)
Article
Fisheries
Benjamin R. LaFrentz, Julio C. Garcia, John P. Shelley
JOURNAL OF FISH DISEASES
(2019)
Article
Fisheries
M. Mabrok, P. Chokmangmeepisarn, B. R. LaFrentz, P. Kayansamruaj, H. T. Dong, C. Rodkhum
Article
Microbiology
Eric K. Pomaranski, Matt J. Griffin, Alvin C. Camus, Abigail R. Armwood, Johnny Shelley, Geoffrey C. Waldbieser, Benjamin R. LaFrentz, Julio C. Garcia, Roy Yanong, Esteban Soto
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2020)
Article
Fisheries
Timothy J. Bruce, Jie Ma, Luke P. Oliver, Evan M. Jones, Benjamin R. LaFrentz, Kenneth D. Cain
JOURNAL OF FISH DISEASES
(2020)
Review
Agriculture, Dairy & Animal Science
Allison L. Wise, Benjamin R. LaFrentz, Anita M. Kelly, Lester H. Khoo, Tingbi Xu, Mark R. Liles, Timothy J. Bruce
Summary: Catfish production is a major aquaculture industry in the Southern United States, but high mortality rates and economic losses are often caused by bacterial pathogens. Co-infections of multiple bacterial fish pathogens may alter infection dynamics and complicate disease management.
Article
Microbiology
Emily M. Churchman, Gianni Parello, Miles D. Lange, Bradley D. Farmer, Benjamin R. LaFrentz, Benjamin H. Beck, Mark R. Liles
Summary: In this study, we report the draft genomes and predicted virulence factors of two isolates of Flavobacterium covae, which are known to cause columnaris disease in teleost fish.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Microbiology
Allison L. Wise, Benjamin R. LaFrentz, Anita M. Kelly, Mark R. Liles, Matt J. Griffin, Benjamin H. Beck, Timothy J. Bruce
Summary: A preliminary assessment was conducted to study the bacterial coinfection of Edwardsiella ictaluri and Flavobacterium covae in juvenile channel catfish. The coinfection challenges showed similar cumulative percent mortality (CPM) to the single dose E. ictaluri challenge, but the onset of peak mortality was delayed in fish exposed to F. covae first. Additionally, catfish exposed to E. ictaluri displayed increased serum lysozyme activity and higher expression of pro-inflammatory cytokines.
Article
Fisheries
Uthpala Padeniya, D. Allen Davis, Mark R. Liles, Stacey A. LaFrentz, Benjamin R. LaFrentz, Craig A. Shoemaker, Benjamin H. Beck, Daniel E. Wells, Timothy J. Bruce
Summary: Biofloc technology is a rearing technique that maintains water quality by manipulating carbon and nitrogen levels. This study evaluated the use of two probiotics in a biofloc system for Nile tilapia culture and found that probiotics can be beneficial as a dietary supplement for tilapia reared in biofloc systems.
JOURNAL OF FISH DISEASES
(2023)
Article
Microbiology
Benjamin R. LaFrentz, Stacey A. LaFrentz, Benjamin H. Beck, Covadonga R. Arias
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)