Journal
JOURNAL OF APPLIED PHYSIOLOGY
Volume 115, Issue 12, Pages 1788-1795Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/japplphysiol.00312.2013
Keywords
calcium cycling/excitation-contraction coupling; inborn aerobic capacity; myocardial infarction; cardio-protection
Categories
Funding
- Department of Circulation and Medical Imaging at the Norwegian University of Science and Technology
- K. G. Jebsen Foundation
- Norwegian Council on Cardiovascular Disease
- National Center for Research Resources [R24 RR-017718]
- National Institutes of Health (NIH) [ROD012098A]
- NIH [RO1 DK-077200]
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Maximal oxygen uptake (Vo(2max)) is a strong prognostic marker for morbidity and mortality, but the cardio-protective effect of high inborn Vo(2max) remains unresolved. We aimed to investigate whether rats with high inborn Vo(2max) yield cardio-protection after myocardial infarction (MI) compared with rats with low inborn Vo(2max). Rats breed for high capacity of running (HCR) or low capacity of running (LCR) were randomized into HCR-SH (sham), HCR-MI, LCR-SH, and LCR-MI. Vo(2max) was lower in HCR-MI and LCR-MI compared with respective sham (P < 0.01), supported by a loss in global cardiac function, assessed by echocardiography. Fura 2-AM loaded cardiomyocyte experiments revealed that HCR-MI and LCR-MI decreased cardiomyocyte shortening (39%, and 34% reduction, respectively, both P < 0.01), lowered Ca2+ transient amplitude (37%, P < 0.01, and 20% reduction, respectively), and reduced sarcoplasmic reticulum (SR) Ca2+ content (both; 20%, P < 0.01) compared with respective sham. Diastolic Ca2+ cycling was impaired in HCR-MI and LCR-MI evidenced by prolonged time to 50% Ca2+ decay that was partly explained by the 47% (P < 0.01) and 44% (P < 0.05) decrease in SR Ca2+ -ATPase Ca2+ removal, respectively. SR Ca2+ leak increased by 177% in HCR-MI (P < 0.01) and 67% in LCR-MI (P < 0.01), which was abolished by inhibition of Ca2+/calmodulin-dependent protein kinase II. This study demonstrates that the effect of MI in HCR rats was similar or even more pronounced on cardiac-and cardiomyocyte contractile function, as well as on Ca2+ handling properties compared with observations in LCR. Thus our data do not support a cardio-protective effect of higher inborn aerobic capacity.
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