Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 24, Issue 5, Pages 1279-1286Publisher
SPRINGER
DOI: 10.1007/s10811-011-9776-y
Keywords
Antioxidant; Cold stress; Galloyl glucose; Phenolic compound; Shikimate pathway; Spirogyra
Funding
- Korean Research Foundation [KRF 2010020701]
- Marine and Extreme Genome Research Center Program of the Ministry of Land, Transportation and Maritime Affairs, Republic of Korea
- Ministry of Land, Transport and Maritime Affairs, Korea
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The green alga Spirogyra varians accumulated antioxidative compounds in response to cold stress. When the algae were transferred from 20A degrees C to 4A degrees C, the amount of phenolic contents and flavonoids in the cell increased 17 times and 30 times, respectively, in 2 months. At this time, the radical scavenging activity of the methanolic extract of S. varians was 238 times higher than that of initial culture. To identify the responsible antioxidants, the methanolic extract was obtained from the algae grown at 4A degrees C. HPLC analysis of the extract showed six compounds newly produced or increased over time. Four of the compounds were successfully purified, and the structures were identified using H-1 NMR spectroscopy. The compounds were galloyl derivatives-methyl gallate, 1-O-Galloyl-beta-d-glucose, 1,2,3,6-tetra-O-Galloyl-beta-d-glucose and 1,2,3,4,6-penta-O-Galloyl-beta-d-glucose which are intermediates of the shikimate pathway.
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