Article
Behavioral Sciences
Emilson Donizete Pereira Jr, Laurival A. De Luca Jr, Jose Vanderlei Menani, Carina Aparecida Fabricio Andrade
Summary: The study found that spontaneously hypertensive rats (SHRs) have enhanced responses to sweet taste and increased aversive responses to bitter taste, which are independent of challenges in bodily fluid balance.
Article
Medicine, Research & Experimental
Weiquan Zeng, Xiuli Zhang, Yao Lu, Ying Wen, Qiurong Xie, Xuan Yang, Shuyu He, Zhi Guo, Jiapeng Li, Aling Shen, Jun Peng
Summary: This study investigates the effect of neferine on hypertensive vascular remodeling and its underlying mechanisms. The results demonstrate that neferine can effectively reduce blood pressure, pulse wave velocity, and abdominal aortic thickening in hypertensive rats. It also suppresses the abnormal activation of PI3K/AKT and TGF-beta/Smad2/3 signaling pathways.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Nutrition & Dietetics
Shohei Yamaguchi, Yuzumi Hayasaka, Miho Suzuki, Wenhao Wang, Masahiro Koyama, Yasuko Nagasaka, Kozo Nakamura
Summary: This study demonstrates that low-dose orally administered ACh can lower blood pressure in spontaneously hypertensive rats by acting on gastrointestinal M3 muscarinic ACh receptors to increase afferent vagal nerve activity, resulting in decreased sympathetic nervous activity and noradrenaline release.
Article
Immunology
Xiaohua Li, Changhong Ren, Sijie Li, Wenbo Zhao, Peifu Wang, Xunming Ji
Summary: This study investigates the effect of limb remote ischemic conditioning (LRIC) on blood pressure regulation in spontaneously hypertensive rats (SHR) by modulating the hypertensive inflammatory response. The results demonstrate that LRIC lowers blood pressure in SHR by reducing the percentage of monocytes and CD8 T cells, as well as decreasing pro-inflammatory cytokine levels.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Victor Gustavo Balera Brito, Mariana Sousa Patrocinio, Ayna Emanuelli Alves Barreto, Sabrina Cruz Tfaile Frasnelli, Vanessa Soares Lara, Carlos Ferreira Santos, Sandra Helena Penha Oliveira
Summary: The study demonstrated that Telmisartan (TELM) inhibited osteogenic differentiation and mineralization of SHR BMSC, favoring an adipogenic phenotype due to Pparg upregulation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yuta Okamura, Ryo Niijima, Satoshi Kameshima, Tomoko Kodama, Kosuke Otani, Muneyoshi Okada, Hideyuki Yamawaki
Summary: Hypertension is a major risk factor for cardiovascular diseases, caused by various abnormalities including blood vessel contractibility. This study investigated the effects of human omentin-1 on hypertensive complications in aged SHR. It was found that omentin-1 tended to improve left ventricular and renal failure in SHR, indicating its potential therapeutic role in hypertensive complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Atsunori Yamamoto, Kosuke Otani, Muneyoshi Okada, Hideyuki Yamawaki
Summary: The study revealed that the increased protein expression of CMKLR1 in the PVN of rats with hypertension is at least partly responsible for the systemic hypertension.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Behavioral Sciences
E. D. Pereira, A. M. Faria, G. M. F. Andrade-Franze, J. Menani, L. A. De Luca, C. A. F. Andrade
Summary: SHRs ingest more NaCl than normotensive rats, with increased hedonic responses to intra-oral infusions of NaCl. Treatment with diuretics and an ACE blocker increased hedonic responses in normotensive rats but had no effect on SHRs. Central AT1 receptors play a role in increasing NaCl intake and palatability in SHRs.
HORMONES AND BEHAVIOR
(2021)
Article
Neurosciences
Hua Zhang, Jing-Jing Zhou, Jian-Ying Shao, Zhao-Fu Sheng, Jingxiong Wang, Peiru Zheng, Xunlei Kang, Zhenguo Liu, Zixi Jack Cheng, David D. Kline, De -Pei Li
Summary: Elevated levels of CRH and CRHR1 in the PVN are associated with increased sympathetic outflow in primary hypertension, potentially contributing to the hyperactivity of PVN presympathetic neurons.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Pharmacology & Pharmacy
Yanan Li, Dadi Xie, Luxi Li, Pei Jiang
Summary: This study aimed to explore the metabolic changes induced by hypertension at different ages. The results showed that spontaneously hypertensive rats (SHR) at 5 weeks old and 32 weeks old had different metabolic characteristics, mainly involving amino acid metabolism, glycolysis, lipid metabolism, and amino acid metabolism.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2023)
Article
Medicine, Research & Experimental
Qingqing Hou, Shiming Zhang, Yuan Li, Huanjun Wang, Dan Zhang, Dongmei Qi, Yunlun Li, Haiqiang Jiang
Summary: This study aimed to investigate the association of lipid metabolites, circadian genes, and lipid metabolism associated genes in spontaneously hypertensive rats. Through lipidomics and gene expression analysis, it was found that SHR exhibited significant differences in lipid metabolites levels compared to WKY, as well as disturbances in circadian and lipid metabolism associated gene expressions. Moreover, a strong association between the levels of lipid metabolites and circadian genes and lipid metabolism associated genes was identified.
Article
Pharmacology & Pharmacy
Carlos Jose Dias, Herikson Araujo Costa, Carlos Alberto Alves Dias-Filho, Andressa Coelho Ferreira, Bruno Rodrigues, Maria Claudia Irigoyen, Antonio Carlos Romao Borges, Vicenilma de Andadre Martins, Flavia Castello Branco Vidal, Rachel Melo Ribeiro, Natalino Salgado Filho, Cristiano T. Mostarda
Summary: The use of carvacrol in spontaneously hypertensive rats (SHR) showed antihypertensive effects and increased expression of MAS receptors in kidney tissue.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Zhi-Bin Wang, Shi-Long Jiang, Shao-Bo Liu, Jing-Bo Peng, Shuo Hu, Xu Wang, Wei Zhuo, Tong Liu, Ji-Wei Guo, Hong-Hao Zhou, Zhi-Quan Yang, Xiao-Yuan Mao, Zhao-Qian Liu
Summary: This study evaluated the effect of artichoke bud extract (ABE) on heart tissue metabolomics of hypertensive rats. The results showed that artichoke significantly lowered blood pressure in hypertensive rats and affected the levels of eight metabolites. This suggests that ABE may play a role in affecting oxidative stress and purine, nicotinate, and nicotinamide metabolism.
Article
Biology
Leonardo M. T. de Rezende, Leandro C. Brito, Anselmo G. Moura, Alexandre J. L. D. Costa, Tiago F. Leal, Evandro S. Favarato, Lukiya S. C. Favarato, Antonio J. Natali, Candido C. Coimbra, Thales N. Primola-Gomes
Summary: This study found that 16-week-old SHR rats showed higher body temperature during the activity phase, while other circadian rhythm parameters did not change across aging.
JOURNAL OF THERMAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jiang Yan, Yu Honglei, Wu Yun, Dong Sheng, He Yun, Zhang Anhua, Feng Na, Lu Min, Shi Dandan, Wang Jing, Tang Junming, Zhang Wenjun, He Xiju
Summary: This study found that long-term administration of puerarin can significantly improve cardiac function and myocardial morphology in spontaneously hypertensive rats. It reduces the expression of proteins related to myocardial remodeling, alleviates oxidative stress and calcium overload. Further experiments showed that puerarin improves myocardial remodeling by inhibiting the TRPC6-CaN-NFATc3 signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biology
Ana Claudia Ervolino da Silva, Fabio Roberto de Souza Batista, Jaqueline Suemi Hassumi, Leticia Pitol Palin, Naara Gabriela Monteiro, Paula Buzo Frigerio, Roberta Okamoto
Summary: Further studies are needed to evaluate the action of bisphosphonates on the bone repair process in the jaws. This study aimed to evaluate the peri-implant repair of rats with estrogen deficiency and metabolic syndrome treated with risedronate sodium.
Article
Chemistry, Physical
Pier Paolo Poli, Lais Kawamata de Jesus, Ulisses Ribeiro Campos Dayube, Henrique Hadad, Caroline Loureiro, Fernando Yamamoto Chiba, Thayane Silveira Mata Furtado, Maisa Pereira Silva, Roberta Okamoto, Carlo Maiorana, Paulo Sergio Perri de Carvalho, Francisley Avila Souza
Summary: This study evaluates the effects of photobiomodulation therapy on the peri-implant bone healing of implants with different surface treatments. The results showed that PBM therapy had a significant positive effect on the machined surface implants.
Article
Biology
Pedro Henrique Silva Gomes-Ferreira, Paula Buzo Frigerio, Juliana de Moura, Nathalia Dantas Duarte, Danila de Oliveira, Joseph Deering, Kathryn Grandfield, Roberta Okamoto
Summary: The study aimed to evaluate the effects of vitamin D alone or in combination with teriparatide on peri-implant bone tissue in orchiectomized rats. Through an in vivo study, it was found that treatment with vitamin D and teriparatide together can increase the volume of bone tissue around the implant and improve bone quality. However, given the increasing population with bone metabolism disorders seeking dental implant rehabilitation treatment, the verification of medications that promote an increase in bone density is essential.
Article
Biochemistry & Molecular Biology
Denise Weffort, Leticia F. Adolpho, Alann T. P. Souza, Gileade P. Freitas, Helena B. Lopes, Fabiola S. Oliveira, Rayana L. Bighetti-Trevisan, Leticia Pitol-Palin, Doris H. Matsushita, Roberta Okamoto, Marcio M. Beloti, Adalberto L. Rosa
Summary: Type 1 (T1DM) and type 2 (T2DM) diabetes mellitus affect glucose metabolism and cause bone damage through various mechanisms, including the impact on osteoblasts. This study examined the osteoblast differentiation of mesenchymal stem cells (MSCs) from rats with T1DM or T2DM and the effects of removing hyperglycemia on the osteogenic potential of these cells. The results showed that both T1DM and T2DM inhibited the osteoblast differentiation of MSCs cultured in hyperglycemic media, with T1DM having a more significant effect. Restoring normoglycemic conditions partially recovered the osteogenic potential of MSCs from rats with T1DM but not with T2DM. These findings highlight the need for targeted therapies for T1DM- or T2DM-induced bone loss, as both conditions disrupt osteoblast differentiation at different levels and likely through different mechanisms.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Dimitra Athanasiadou, Nadeen Meshry, Naara G. Monteiro, Ana C. Ervolino-Silva, Ryan Lee Chan, Christopher A. McCulloch, Roberta Okamoto, Karina M. M. Carneiro
Summary: DNA-based biomaterials have potential as biodegradable scaffolds for bone repair due to their ability to bind Ca2+ and promote HAP growth. This study describes the design and synthesis of DNA hydrogels, their interactions with osteogenic cell lines, and their promotion of new bone formation in rat calvarial wounds.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Polymer Science
Lilian Caldas Quirino, Pedro Henrique de Azambuja Carvalho, Renato Torres Augusto Neto, Cassio Amaro Comachio, Naara Gabriela Monteiro, Ana Claudia Ervolino-Silva, Roberta Okamoto, Valfrido Antonio Pereira-Filho
Summary: This study evaluated the bone repair in critical defects using a polydioxanone membrane (Plenum((R)) Guide) and a collagen-based membrane (Bio-Gide((R))) in rats. The results showed that the Plenum((R)) Guide group had better bone regeneration and gene expression, but there was no significant difference between the two groups in histometric and micro-CT analyses. Therefore, the absorbable polydioxanone membrane is an effective guided bone regeneration membrane.
Article
Engineering, Biomedical
Juliani Caroline Ribeiro de Araujo, Leonardo Alvares Sobral Silva, Vinicius Almeida de Barros Lima, Tiago Moreira Bastos Campos, Paulo Noronha Lisboa Filho, Roberta Okamoto, Luana Marotta Reis de Vasconcellos
Summary: With increasing life expectancy, the prevalence of osteoporosis has risen, leading to a search for strategies to regenerate bone tissue. The sonochemical technique was used to functionalize bioglass, resulting in modified surface characteristics. In vitro tests showed favorable cell adhesion and differentiation, with no cytotoxicity observed. Animals treated with teriparatide demonstrated new bone formation, indicating positive effects on bone tissue. Further studies are required to investigate the long-term effects of the interaction between bioglass and teriparatide.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Cell Biology
Debora de Souza Ferreira Savio, Lucia Moitrel Pequeno da Silva, Gabriel Guerra David Reis, Ricardo Junior Denardi, Natacha Malu Miranda da Costa, Flavia Aparecida Chaves Furlaneto, Sergio Luis Scombatti de Souza, Carlos Fernando de Almeida Barros Mourao, Richard J. Miron, Roberta Okamoto, Michel Reis Messora
Summary: After tooth loss, bone remodeling can cause significant decreases in bone height and thickness, which can compromise the success of dental implants. Leukocyte-platelet-rich fibrin (L-PRF) has shown potential in promoting tissue regeneration and several protocols have been proposed to improve its regenerative potential. In this study, different centrifugation protocols were evaluated for their effectiveness in bone healing in rat calvaria defects. The results showed that platelet aggregates, especially with horizontal centrifugation, improve bone neoformation and have more biological potential for bone healing.
Article
Engineering, Biomedical
Gabriel Mulinari-Santos, Jaqueline Silva dos Santos, Igor Lebedenco Kitagawa, Fabio Roberto de Souza Batista, Paulo Roberto Botacin, Cristina Antoniali, Paulo Noronha Lisboa-Filho, Roberta Okamoto
Summary: Hypertension and estrogen deficiency can impair osseointegration, and the use of antihypertensive drugs and alendronate sodium can enhance bone healing. However, estrogen deficiency still affects bone integration even with alendronate coating.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Carolina Mendonca de Almeida Malzoni, Elton Carlos Pichotano, Luiz Guilherme Freitas de Paula, Ricardo Violante de Souza, Roberta Okamoto, Rupert S. Austin, Elcio Marcantonio Jr, Rafael Scaf de Molon, Daniela Leal Zandim-Barcelos
Summary: The combination of leukocyte and platelet-rich fibrin (L-PRF) and deproteinized bovine bone mineral (DBBM) was found to enhance bone regeneration and improve implant stability in maxillary sinus augmentation procedures, allowing for faster implant placement.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Biochemistry & Molecular Biology
Bruna Kaori Namba Inoue, Laura Vidoto Paludetto, Naara Gabriela Monteiro, Fabio Roberto de Souza Batista, Igor Lebedenco Kitagawa, Roberto Santana da Silva, Cristina Antoniali, Paulo Noronha Lisboa Filho, Roberta Okamoto
Summary: Postmenopausal osteoporosis and poor dietary habits can lead to overweightness and obesity. This study found that nitric oxide has the potential to improve peri-implant conditions, especially at low concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Ana Flavia Piquera Santos, Lara Cristina Cunha Cervantes, Roberta Okamoto, Antonio Carlos Guastaldi, Thallita Pereira Queiroz, Layla Panahipour, Reinhard Gruber, Francisley avila Souza
Summary: This study investigated the effect of surface modifications on osteogenic and stromal cells. The results showed that laser treatment enhanced the hydrophilicity of titanium surface, while hydroxyapatite coating reduced cell viability and induced the release of inflammatory cytokines.
APPLIED SCIENCES-BASEL
(2023)
Article
Medicine, General & Internal
Anderson Maikon de Souza Santos, Rodrigo dos Santos Pereira, Pietro Montemezzi, Rafael Coutinho Mello-Machado, Roberta Okamoto, Roberto Sacco, Paulo Noronha Lisboa-Filho, Michel Reis Messora, Carlos Fernando Mourao, Eduardo Hochuli-Vieira
Summary: The study aimed to assess the efficacy of using Raloxifene with ultrasonic processing to enhance Bio-Oss & REG; for maxillary sinus bone height reconstruction. Results indicated that while sonicated Bio-Oss & REG; performed comparably to Bio-Oss & REG; alone, the addition of Raloxifene led to an unexpected delay in bone repair. These findings stress the importance of histological evaluation for accurate bone repair assessment.
MEDICINA-LITHUANIA
(2023)
Article
Chemistry, Physical
Paula Buzo Frigerio, Pedro Henrique Silva Gomes-Ferreira, Fabio Roberto De Souza Batista Juliana Moura, Idelmo Rangel Garcia Junior, Daniele Botticelli, Paulo Noronha Lisboa-Filho, Roberta Okamoto
Summary: This study evaluated the behavior of repaired bone in rats after extraction, focusing on the use of PTH (teriparatide) and Biogran (R) (a biomaterial). The results showed that the use of Biogran (R) with PTH improved bone quality in healthy rats, but did not have significant effects in orchiectomized rats. Further research is needed to determine more effective doses for postoperative bone formation.
Article
Multidisciplinary Sciences
Chiara Micheletti, Pedro Henrique Silva Gomes-Ferreira, Travis Casagrande, Paulo Noronha Lisboa-Filho, Roberta Okamoto, Kathryn Grandfield
Summary: This paper presents a workflow for characterizing the interface between newly formed bone and bioglass at the nanoscale, utilizing scanning and transmission electron microscopy techniques. The study provides unprecedented resolution of the bone-BG interface and proposes a method for three-dimensional visualization using electron tomography.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2021)