Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 116, Issue 4, Pages 934-941Publisher
WILEY
DOI: 10.1111/jam.12434
Keywords
MALDI-TOF MS; Sepsityper; sepsis; bacteraemia; blood cultures
Categories
Funding
- BD Diagnostics
- TREK Diagnostic Systems
- Bruker Daltonics
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Aims (i) Evaluation of delayed time to blood culture extraction by the Sepsityper kit and impact of shipping pellets off-site for MALDI-TOF MS analysis. (ii) Comparison of Sepsityper and laboratory-developed extraction methods from a literature review. Methods and Results Using two blood culture systems (BD BACTEC and VersaTREK), we extracted 411 positive blood cultures using the Sepsityper kit to mimic a potential protocol for institutions without a MALDI-TOF MS. Extracted pellets were shipped and analysed on the Bruker UltraflexIII. Successful extraction of 358 (87 center dot 1%) samples was determined by the presence of detectable proteins. MALDI-TOF MS correctly identified 332 (80 center dot 8%) samples. Conclusions Delayed time to extraction did not affect Sepsityper extraction or MALDI-TOF MS accuracy. The extracted pellets remain stable and provide accurate results by MALDI-TOF MS when shipped at room temperature to off-site reference laboratories. Significance and Impact of the Study This is the first study to show that institutions without a MALDI-TOF MS can take advantage of this innovative technology by shipping a volume of blood to an off-site laboratory for extraction and MALDI-TOF MS analysis. We also performed a literature review to compare various extraction methods.
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