Article
Immunology
Zhenpeng Li, Hongxia Guan, Wei Wang, He Gao, Weihong Feng, Jie Li, Baowei Diao, Hongqun Zhao, Biao Kan, Jingyun Zhang
Summary: A multiplex TaqMan-based real-time PCR assay was developed to detect and differentiate Vibrio cholerae and Vibrio parahaemolyticus directly from human fecal specimens with high sensitivity and specificity. The BD MAX assay showed high overall agreement with conventional real-time PCR, making it an effective detection method for these pathogens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Yong Yan, Li Zhan, Guoying Zhu, Junyan Zhang, Ping Li, Lixia Chen, Peiyan He, Jianyong Luo, Zhongwen Chen
Summary: A novel multiplex assay for rapid detection of Vibrio cholerae serogroup and cholera toxin toxigenicity was developed. The assay showed good diagnostic sensitivities and is suitable for rapid identification and screening of large specimens.
Article
Food Science & Technology
Tong Wang, Shuo Wan, Jian-Lian Huang, Zhi-Hai Sui, Cui-Juan Gao, Yun-Guo Liu
Summary: This study investigated and analyzed the pollution status of pathogenic Vibrio in marine products in China, and found that pathogenic Vibrio contamination existed in different regions, different types of aquatic products, and different sampling locations. The detection rate of the pathogen was statistically analyzed. This provides a theoretical basis for food safety supervision departments to develop targeted prevention and control measures.
JOURNAL OF FOOD QUALITY
(2022)
Article
Environmental Sciences
Candice A. Thorstenson, Matthias S. Ullrich
Summary: The potential spread of infectious diseases in response to climate change and rising sea surface temperatures in temperate regions has raised concerns over the past few decades. Different species of Vibrio, particularly Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus, have been correlated with human health risks in relation to extreme heat waves in the North Atlantic and North Sea regions. Studying the behavior and ecology of both pathogenic and non-pathogenic strains of these bacteria can provide insights into their native microbiome and how they disperse in coastal regions.
FRONTIERS IN MARINE SCIENCE
(2021)
Review
Environmental Sciences
Greta Kalvaitiene, Diana Vaiciute, Martynas Bucas, Greta Gyraite, Marija Katarzyte
Summary: Waterborne pathogenic bacteria, such as faecal indicator bacteria and potentially pathogenic Vibrio, pose a global concern for waterborne diseases. A systematic review found that brown algae and seagrasses had the highest quantities of faecal indicator bacteria, while red algae had the highest quantities of Vibrio bacteria. The research on macrophytes focused mainly on brown algae, with green algae being the least studied. Further research is needed to understand the role of potentially pathogenic Vibrio and faecal indicator bacteria with macrophytes and their microbiome in the coastal marine environment.
MARINE POLLUTION BULLETIN
(2023)
Article
Microbiology
Hector Carmona-Salido, Naiel Bisharat, Carmen Amaro
Summary: A newly developed PCR method based on comparative genomic study has been described for rapid identification and epidemiological studies of Vibrio vulnificus lineage 3. The analysis also revealed a genetic duplication in the L3 strains, potentially linked to their unique ability to produce septicemia outbreaks.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Pei Wang, Lei Liao, Chao Ma, Xue Zhang, Junwei Yu, Longyu Yi, Xin Liu, Hui Shen, Song Gao, Qunwei Lu
Summary: The duplex detection biosensor based on RPA technology and LFS strips showed good specificity and high sensitivity for Vibrio cholerae and Vibrio vulnificus. It is simple and fast, suitable for on-site detection, and capable of simultaneous detection of these two important foodborne bacteria.
Article
Microbiology
James W. Conrad, Valerie J. Harwood
Summary: This study reveals that sewage can significantly increase the density of Vibrio vulnificus populations and affect the transcription of genes associated with virulence, suggesting an increased risk of V. vulnificus infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Medicine, General & Internal
Kristin Walter
Summary: This article describes the signs, symptoms, diagnosis, treatment, and prevention strategies for Vibrio vulnificus infection.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2023)
Article
Environmental Sciences
Jessica A. Bullington, Abigail R. Golder, Grieg F. Steward, Margaret A. McManus, Anna B. Neuheimer, Brian T. Glazer, Olivia D. Nigro, Craig E. Nelson
Summary: The south shore of O`ahu, Hawaii, which is one of the most popular coastal tourism areas in the United States, has a high incidence of recreational waterborne disease. Researchers developed a statistical model to predict the dynamics of pathogenic bacterium Vibrio vulnificus in the estuarine Ala Wai Canal in Honolulu. The model utilized environmental measurements and showed that factors such as rainfall, air temperature, water temperature, nitrate plus nitrite, and dissolved organic matter had significant influence on V. vulnificus concentration. Real-time predictions of V. vulnificus concentration can be made using this model applied to water quality measurements.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Sadok Khouadja, Ana Roque, Mar Gonzalez, Dolors Furones
Summary: In this study, we investigated the presence of Vibrio cholerae virulence genes in Vibrio alginolyticus isolates and found that some of the V. alginolyticus strains might have derived their virulence genes from V. cholerae, making them a possible reservoir of V. cholerae virulence genes.
JOURNAL OF WATER AND HEALTH
(2022)
Article
Microbiology
Stavros Droubogiannis, Lydia Pavlidi, Maria Ioanna Tsertou, Constantina Kokkari, Dimitrios Skliros, Emmanouil Flemetakis, Pantelis Katharios
Summary: A novel species of Siphoviridae family, Vibrio phage Artemius, which can specifically infect V. alginolyticus strains, was reported. It efficiently reduces the growth of the host bacterium as assessed by an in vitro lysis assay.
Article
Chemistry, Medicinal
Katsushiro Miyamoto, Hiroaki Kawano, Naoko Okai, Takeshi Hiromoto, Nao Miyano, Koji Tomoo, Takahiro Tsuchiya, Jun Komano, Tomotaka Tanabe, Tatsuya Funahashi, Hiroshi Tsujibo
Summary: Vibrio vulnificus M2799 secretes the catecholate siderophore vulnibactin to capture ferric ions for growth, utilizing a ferric-import system composed of various proteins including an isochorismate synthase and an outer membrane receptor. In infectious diseases, periplasmic-binding proteins and ferric-chelate reductases involved in the ferric-siderophore utilization system could be potential targets for drug discovery.
Article
Immunology
Jia-Xin Zhang, Yuan Yuan, Qing-hua Hu, Da-zhi Jin, Yao Bai, Wen-Wen Xin, Lin Kang, Jing-Lin Wang
Summary: By integrating whole genome sequencing, genome-wide association studies, and genome-wide epistasis studies, we identified six genes associated with the pathogenicity of V. vulnificus. These genes are involved in nucleotide/amino acid transport and metabolism, cell membrane biogenesis, signal transduction mechanisms, and protein turnover. We also discovered two ecological groups of V. vulnificus with distinct biotic and abiotic factors and ecological strategies. This study provides insights into the pathogenic mechanisms and evolution of V. vulnificus, offering a foundation for the development of new vaccines and therapeutic targets.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Fisheries
Mirjam Boonstra, Belen Fouz, Betty van Gelderen, Inger Dalsgaard, Lone Madsen, Eva Jansson, Carmen Amaro, Olga Haenen
Summary: The study aimed to evaluate the accuracy of MALDI-TOF mass spectrometry in the identification of SerE from V. vulnificus. Serovar-specific peaks were found for SerE at approximately 3098 Da and 4045 Da, for SerA at approximately 3085 Da and 4037 Da, and for SerI at approximately 3085 Da and 4044 Da. Therefore, MALDI-TOF can be used to identify SerE and aid in the identification of other serovars, potentially preventing zoonotic transmission.
JOURNAL OF FISH DISEASES
(2023)
Article
Biotechnology & Applied Microbiology
Kevin Esteves, Dominique Hervio-Heath, Thomas Mosser, Claire Rodier, Marie-George Tournoud, Estelle Jumas-Bilak, Rita R. Colwell, Patrick Monfort
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Microbiology
Kevin Esteves, Thomas Mosser, Fabien Aujoulat, Dominique Hervio-Heath, Patrick Monfort, Estelle Jumas-Bilak
FRONTIERS IN MICROBIOLOGY
(2015)
Article
Multidisciplinary Sciences
Jaime Martinez-Urtaza, Andy Powell, Josep Jansa, Jose Luis Castro Rey, Oscar Paz Montero, Marta Garcia Campello, Ma Jose Zamora Lopez, Anxela Pousa, Ma Jose Faraldo Valles, Joaquin Trinanes, Domique Hervio-Heath, William Keay, Amanda Bayley, Rachel Hartnell, Craig Baker-Austin
Article
Microbiology
Carina Pretzer, Irina S. Druzhinina, Carmen Amaro, Eva Benediktsdottir, Ingela Hedenstroem, Dominique Hervio-Heath, Steliana Huhulescu, Franciska M. Schets, Andreas H. Farnleitner, Alexander K. T. Kirschner
ENVIRONMENTAL MICROBIOLOGY
(2017)
Article
Food Science & Technology
R. E. Hartnell, L. Stockley, W. Keay, J. -P. Rosec, D. Hervio-Heath, H. Van den Berg, F. Leoni, D. Ottaviani, U. Henigman, S. Denayer, B. Serbruyns, F. Georgsson, G. Krumova-Valcheva, E. Gyurova, C. Blanco, S. Copin, E. Strauch, K. Wieczorek, M. Lopatek, A. Britova, G. Hardouin, B. Lombard, P. In't Veld, A. Leclercq, C. Baker-Austin
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
A. Tall, D. Hervio-Heath, A. Teillon, C. Boisset-Helbert, R. Delesmont, J. Bodilis, A. Touron-Bodilis
JOURNAL OF APPLIED MICROBIOLOGY
(2013)
Article
Microbiology
Alain Rince, Charlotte Baliere, Dominique Hervio-Heath, Joelle Cozien, Solen Lozach, Sylvain Parnaudeau, Francoise S. Le Guyader, Simon Le Hello, Jean-Christophe Giard, Nicolas Sauvageot, Abdellah Benachour, Sofia Strubbia, Michele Gourmelon
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Chemistry, Multidisciplinary
Delphine Passerini, Florian Fecamp, Laetitia Marchand, Laetitia Kolypczuk, Sandrine Bonnetot, Corinne Sinquin, Veronique Verrez-Bagnis, Dominique Hervio-Heath, Sylvia Colliec-Jouault, Christine Delbarre-Ladrat
APPLIED SCIENCES-BASEL
(2019)
Article
Microbiology
Kevin Drouet, Cecile Jauzein, Dominique Herviot-Heath, Saeed Hariri, Aitor Laza-Martinez, Cyrielle Lecadet, Martin Plus, Sergio Seoane, Marc Sourisseau, Rodolphe Lemee, Raffaele Siano
Summary: During a sampling campaign in August to September 2018, microscope analysis revealed that the Ostreopsis cf. siamensis mainly thrived in the southeast of Bay of Biscay where optimal temperatures promoted its blooms. Quantitative PCR analyses showed its presence across almost the whole bay and extending to the western English Channel. Time-series eDNA data collected suggested the species occurrence from April to September in the bay.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Francoise Vincent-Hubert, Candice Wacrenier, Benjamin Morga, Solen Lozach, Emmanuelle Quenot, Mickael Mege, Cyrielle Lecadet, Michele Gourmelon, Dominique Hervio-Heath, Francoise S. Le Guyader
Summary: The study developed a passive sampling method using polymer membranes to detect viruses and bacteria in seawater, which proved to be promising for early warning system in oyster farming areas and improving knowledge on disease occurrence timing and frequency. Different membranes showed varying degrees of effectiveness in detecting specific microorganisms, with some showing seasonal distribution patterns.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Damien Reveillon, Veronique Savar, Estelle Schaefer, Julien Cheve, Marie-Pierre Halm-Lemeille, Dominique Hervio-Heath, Marie-Agnes Travers, Eric Abadie, Jean-Luc Rolland, Philipp Hess
Summary: Tetrodotoxins have been identified in European shellfish at relatively low levels, with a lack of sufficient data on occurrence. Studies on shellfish and bacterial strains found no TTX analog in bacteria, despite its presence in shellfish. Survey of bivalves in French production areas revealed fluctuating concentrations of TTX, confirming the complexity of the link between bacteria, bivalves, and TTX.
Article
Microbiology
Marion Soree, Francois Delavat, Christophe Lambert, Solen Lozach, Mathias Papin, Bruno Petton, Delphine Passerini, Lionel Degremont, Dominique Hervio Heath
Summary: The study revealed that oysters grown in controlled environments accumulated more Vibrio parahaemolyticus than those grown in intertidal environments, possibly due to immune priming in the latter. However, both types of oysters showed similar depuration rates for V. parahaemolyticus.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Fisheries
Marion Soree, Anna Le Meleder, Elise Maurouard, Solen Lozach, Mathias Papin, Christophe Stavrakakis, Corinne Audemard, Dominique Hervio Heath, Lionel Degremont
Summary: In this study, we evaluated the impact of ploidy in Crassostrea gigas oysters on contamination with indigenous Vibrio parahaemolyticus (Vp) and on the accumulation and depuration of Vp in laboratory experiments. The results showed that ploidy did not significantly influence contamination by indigenous Vp, although triploid oysters had a lower contamination trend in November. The pathogenic profiles of indigenous Vp varied between seasons, and depuration was higher during the summer months.
Article
Biotechnology & Applied Microbiology
Marion Soree, Laetitia Kolypczuk, Emilie Hadjiev, Solen Lozach, Veronique Verrez-Bagnis, Christine Delbarre-Ladrat, Dominique Hervio Heath, Delphine Passerini
Summary: This study investigated the use of marine lactic acid bacteria (LAB) for reducing Vibrio parahaemolyticus levels during the oyster depuration process. In vitro assays were conducted to evaluate the inhibitory effect of 30 marine LAB strains against V. parahaemolyticus strains. Three LAB strains were selected for reducing V. parahaemolyticus levels during oyster depuration. The results showed that the addition of LAB strains significantly improved the reduction of V. parahaemolyticus levels.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Khadidja Z. Klouch, Sabine Schmidt, Francoise Andrieux-Loyer, Mickael Le Gac, Dominique Hervio-Heath, Zujaila N. Qui-Minet, Julien Quere, Estelle Bigeard, Laure Guillou, Raffaele Siano
FEMS MICROBIOLOGY ECOLOGY
(2016)