Article
Biochemistry & Molecular Biology
Kailu Zhou, Yangyang Zhang, Yikai Zhou, Minghao Xu, Shanshan Yu
Summary: Enzymatic transformation of gypenosides provides a new way to obtain more pharmacologically active components. In this study, a gene encoding a glycosyl hydrolase was cloned and expressed to produce gypenoside XVII from ginsenoside Rb1. The purified enzyme showed optimal activity and the product exhibited improved anti-inflammatory effects compared to the precursor.
Review
Food Science & Technology
Hongrong Zhu, Rui Zhang, Zunxi Huang, Junpei Zhou
Summary: In recent years, minor ginsenosides have gained attention for their biological activities, but they are scarce in wild ginseng. Ginsenoside Rb1, a major component, can be converted into minor ginsenosides using beta-glucosidases. This paper reviews the literature on hydrolysis of ginsenoside Rb1 by beta-glucosidases, discussing the enzymatic pathways, characteristics, and molecular mechanisms. The study concludes that beta-glucosidases from bacteria and fungi, mainly from GH1 and GH3 families, hydrolyze ginsenoside Rb1 through specific pathways, and their properties vary in terms of temperature, degradation products, and kinetics. Further research should focus on understanding the molecular mechanisms and improving the performance of beta-glucosidases for applications in the food and pharmaceutical industries.
Article
Biotechnology & Applied Microbiology
Aslihan Kazan, Xihua Hu, Alina Stahl, Heike Frerichs, Irina Smirnova, Ozlem Yesil-Celiktas
Summary: The study developed an alginate-based immobilized enzyme microreactor for the catalytic conversion of ginsenoside Rb1 to more bioactive metabolites, showing enhanced activity compared to conventional approaches. There is potential for the biocatalysis of multiple ginsenosides and pharmaceutically active compounds using this newly developed enzymatic microreactor.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Gereltuya Renchinkhand, Urgamal Magsar, Hyoung Churl Bae, Suk-Ho Choi, Myoung Soo Nam
Summary: Lentilactobacillus buchneri isolated from Korean fermented plant foods can effectively hydrolyze ginsenoside Rb1 into Rd and Rg3. The strain also exhibits higher enzymatic activities compared to other strains, which makes it suitable for the production of functional foods, beverages, cosmetics, and health products.
Article
Food Science & Technology
Shuning Zhong, Mi Yan, Haoyang Zou, Ping Zhao, Haiqing Ye, Tiehua Zhang, Changhui Zhao
Summary: This study demonstrated the binding mechanism of beta-glucosidase with ginsenoside Rb-1, including influencing factors and spectral features. This contributes to a better understanding of this interaction.
FOOD SCIENCE & NUTRITION
(2021)
Article
Biotechnology & Applied Microbiology
Chunlong Yan, Chunyun Hao, Wanzhu Jin, Wei-Wei Dong, Lin-Hu Quan
Summary: The research focused on the genetic and biochemical properties of the beta-glucosidase gene from Enterococcus gallinarum GM2 in rat intestinal bacteria, which effectively converts ginsenoside Rb1 into ginsenoside F2 with a conversion yield of 45%. The study elucidated the relationship between intestinal bacteria and the metabolism of ginsenoside, highlighting the key role of Bgy3 in this process.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2021)
Article
Chemistry, Medicinal
Jee Youn Lee, Hae Young Choi, Chan Sol Park, Dong Hyun Kim, Tae Young Yune
Summary: The study showed that total saponin extract (TSE), ginsenoside Rb1, and compound K derived from red ginseng have potential antinociceptive effects against neuropathic pain, alleviating allodynia and hyperalgesia induced by peripheral and central neuropathic pain. Additionally, the antinociceptive effects of TSE and Rb1 were found to be mediated through the estrogen receptor (ER), and compound K also demonstrated significant alleviation of both types of neuropathic pain.
PHYTOTHERAPY RESEARCH
(2021)
Article
Biochemical Research Methods
Xiuhong Wu, Boquan Qu, Yongkang Liu, Xingxuan Ren, Shaoyan Wang, Yanling Quan
Summary: This study developed an enzyme immobilization method for the efficient biotransformation of ginsenoside Rb1, resulting in increased activity and stability. The immobilized enzyme showed higher immobilization efficiency, a wider optimal pH range, higher thermostability, and greater catalytic efficiency compared to the free enzyme.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Hengmian Sun, Li-Juan Ma, Jian-Bo Wan, Shengqiang Tong
Summary: In this study, four minor saponins were successfully extracted from Panax notoginseng leaves and separated using countercurrent chromatography. The purification process yielded highly pure samples of these minor saponins, facilitating further pharmacological studies.
JOURNAL OF SEPARATION SCIENCE
(2021)
Review
Oncology
Jinlong Liu, Yuchen Wang, Zhun Yu, Guangfu Lv, Xiaowei Huang, He Lin, Chao Ma, Zhe Lin, Peng Qu
Summary: This review summarizes the pharmacological activity and functional mechanisms of Compound K (CK) in cancer treatment, including inhibiting tumor growth by inducing tumor cell apoptosis and differentiation, and regulating tumor microenvironment through suppressing tumor angiogenesis and immunosuppressive cells.
INTEGRATIVE CANCER THERAPIES
(2022)
Article
Genetics & Heredity
Pan Wang, Congcong Tang, Yannan Liu, Jing Yang, Daidi Fan
Summary: This study successfully expressed a thermostable β-glycosidase from Sulfolobus solfataricus in Pichia pastoris and secreted it into fermentation broth. The optimized enzyme showed the highest catalytic efficiency at 80°C and pH 6.0, and its activity was significantly enhanced in the presence of Li+. The recombinant enzyme could completely convert the ginsenoside substrate to the rare ginsenoside Compound K and exhibited extraordinary tolerance against high substrate concentrations.
Article
Medicine, Research & Experimental
Yuguo Li, Jiang Wu, Naiqiang Zhuo
Summary: Ginsenoside compound K (GCK) can reduce cartilage degradation by inhibiting the activation of NLRP3 inflammasome and subsequent pyroptosis. The study also found that GCK can reverse the abnormal expression of chondrocytes caused by the inflammatory cytokine TNF-α and restore normal function.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Li Zhou, Zhong-Kun Li, Cong-Yuan Li, Yue-Qin Liang, Fan Yang
Summary: Ginsenoside compound K (CK) is a major intestinal bacterial metabolite of ginsenosides with anticancer potential, exhibiting effects such as inhibiting proliferation, inducing apoptosis and autophagy. Combining CK with nanocarriers enhances its anticancer efficiency, showing promise for further research on the medicinal value of small molecules like CK.
CHEMICAL BIOLOGY & DRUG DESIGN
(2022)
Article
Biochemical Research Methods
Dayong Zheng, Yang Chu, Shuming Li, Shuiping Zhou, Wei Li, Yuesheng Xie, He Sun
Summary: This study found that borneol can significantly affect the pharmacokinetic parameters of CDDP components in human plasma, increasing the absorption and systemic exposure of ginsenoside Rb-1, ginsenoside Rg(1), and notoginsenoside R-1 by approximately 1.85-3.71 times.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Biochemistry & Molecular Biology
Qian Chen, Dongwen Ren, Luokun Liu, Jingge Xu, Yuzheng Wu, Haiyang Yu, Mengyang Liu, Yi Zhang, Tao Wang
Summary: This study found that ginsenoside compound K (CK) has renoprotective properties in early intervention of diabetic kidney disease (DKD). CK supplementation can improve urine microalbumin and renal morphological abnormalities and play a role through regulating gut microbiota and reducing the levels of histidine-derived microbial metabolite imidazole propionate (IMP) in the serum.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Jun-jie Zhang, Ke-cheng Chen, Yue Zhou, Heng Wei, Meng-han Qi, Zi Wang, Yi-nan Zheng, Ri-xin Chen, Shuang Liu, Wei Li
Summary: The study found that Rg2 could delay brain aging by increasing mitochondrial autophagy flux to maintain mitochondrial function, demonstrating good anti-aging activity.
Article
Chemistry, Medicinal
Jun-nan Hu, Yi-ming Wang, Hao Zhang, Hui-ping Li, Zi Wang, Mei Han, Shen Ren, Shan Tang, Shuang Jiang, Wei Li
Summary: The study found that Scheme B has a protective effect on cisplatin-induced rat intestinal crypt epithelial cell injury. It achieves this by reducing oxidative stress, inhibiting cell apoptosis and inflammation, and repairing intestinal barrier function.
PHYTOTHERAPY RESEARCH
(2023)
Article
Food Science & Technology
Yong-bo Liu, Da-zhong Sun, Ke-cheng Chen, Jun-jie Zhang, Yun-yi Hou, Xu-fei Gao, En-bo Cai, Hong-Yan Zhu, Yi-nan Zheng, Ri-xin Chen, Shuang Liu, Wei Li
Summary: The study demonstrated that ginseng berry saponin (GBS) treatment had protective effects in a mouse brain injury model, improving memory impairment, alleviating cell apoptosis and oxidative stress, and delaying cell aging.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Chemistry, Multidisciplinary
Yue-yang Duan, Xiao-jie Mi, Wen-ya Su, Shan Tang, Shuang Jiang, Zi Wang, Li-Chun Zhao, Wei Li
Summary: The present study demonstrates that TLB has a significant protective effect against cisplatin-induced acute kidney injury. TLB reverses the abnormal renal function indicators, alleviates renal pathological changes, and inhibits renal tissue cell apoptosis caused by cisplatin exposure. Additionally, it is shown that TLB modulates the AKT/MAPK signaling pathway and apoptosis to mitigate cisplatin-induced nephrotoxicity.
Article
Biochemistry & Molecular Biology
Shihan Wang, Li Ji, Dongxue Zhang, Hongye Guo, Yongsheng Wang, Wei Li
Summary: In this study, 1-methylhydantoin cinnamic imides were synthesized and their anti-inflammatory activity was evaluated. In vitro experiments showed that certain compounds effectively inhibited the production of inflammatory molecules and had some cytotoxicity. In vivo experiments demonstrated that these compounds could alleviate ear edema in mice, with compound 5 showing the most significant effect.
Review
Biochemistry & Molecular Biology
Jiabing An, Ivan Steve Nguepi Tsopmejio, Zi Wang, Wei Li
Summary: This review summarizes the relevant literature on peptides from the past decade, including extraction, modification, and synthesis. The unique beneficial effects of peptides on the skin were extensively explored, providing ideas for peptide development and innovation. Additionally, the knowledge foundation for the clinical application of peptides was laid.
Article
Biochemistry & Molecular Biology
Zijing Shang, Meiling Fan, Jingtian Zhang, Zi Wang, Shuang Jiang, Wei Li
Summary: This study evaluated the ameliorative effect and molecular mechanism of red ginseng extract on D-galactose-induced premature ovarian failure using network pharmacology analysis. Ginsenosides, sugar, and amino acid derivatives in red ginseng were identified as important active ingredients. The study constructed a protein-protein interaction network and identified core targets related to premature ovarian failure. In vivo experiments confirmed that red ginseng extract can increase estradiol levels and reduce follicle-stimulating hormone levels by reducing oxidative stress, apoptosis, and senescence signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Plant Sciences
Si-min Qi, Jing-tian Zhang, Hong-yan Zhu, Zi Wang, Wei Li
Summary: Glaucoma is the second most common blindness in the world, affecting the life quality of patients. Traditional Chinese Medicines (TCM) have become an important content in ophthalmology, characterized by more targets and lower toxicity. This review presents an overview of glaucoma pathogenesis and therapeutic effects of TCM, as well as the application of acupuncture.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Plant Sciences
Jing-Tian Zhang, Li -Ya Xie, Qiong Shen, Wei Liu, Ming -Han Li, Rui-Yi Hu, Jun-Nan Hu, Zi Wang, Chen Chen, Wei Li
Summary: This study investigated the regulatory effects of Platycodin D (PD) on neurodegeneration caused by reactive oxidative stress (ROS). In vivo and in vitro experiments demonstrated that PD could improve memory, reduce cell apoptosis and ROS production, and inhibit inflammation. PD enhanced the antioxidant ability by activating AMPK. This study suggests that PD may be a potential pharmaceutical agent to treat ROS-induced neurodegeneration.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Yun-yi Hou, Si-min Qi, Jing Leng, Qiong Shen, Shan Tang, Jing-tian Zhang, Jun-nan Hu, Shuang Jiang, Wei Li
Summary: This study investigated the protective effect of lobetyolin (LBT) against cisplatin-induced cytotoxicity in HEK293 cells. Results showed that LBT improved the viability of HEK293 cells, decreased MDA levels, decreased GSH content, and suppressed ROS levels caused by cisplatin. Molecular docking analysis revealed a strong binding affinity between LBT and the NF-κB protein. LBT also inhibited inflammation and apoptosis in HEK293 cells.
JOURNAL OF NATURAL MEDICINES
(2023)
Review
Biochemistry & Molecular Biology
Shuai Zhang, Chuanbo Ding, Xinglong Liu, Yingchun Zhao, Qiteng Ding, Shuwen Sun, Jinping Zhang, Jiali Yang, Wencong Liu, Wei Li
Summary: This review summarizes the extraction, isolation and purification, structural characteristics, and biological activities of ginseng, American ginseng, and Panax notoginseng polysaccharides. These polysaccharides have various biological activities including antioxidants, antitumor, immunomodulatory, antidiabetic, intestinal protection, and skin repair.
Article
Food Science & Technology
Jian-Qiang Wang, Xiang-Xiang Liu, Jun-Jie Zhang, Chao Jiang, Si-Wen Zheng, Zi Wang, Da-Yong Li, Wei Li, Dong-fang Shi
Summary: This study investigated the potential protective mechanism of arabinogalactan (AG) on acute kidney injury (AKI) induced by cisplatin in mice. The results showed that pretreatment with AG could reduce kidney injury and oxidative stress induced by cisplatin, as well as restore the expression of anti-apoptotic proteins and decrease the expression of pro-apoptotic proteins in renal tissues.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Plant Sciences
Qiong Shen, Si-min Qi, Jing-tian Zhang, Min-han Li, Ying-ping Wang, Zi Wang, Wei Li
Summary: This study aimed to investigate the improvement effects and regulatory mechanisms of Platycodin D (PD) on kidney injury in diabetic nephropathy. The results showed that PD can lower blood glucose and insulin resistance levels, improve lipid levels and renal function, and inhibit the development of diabetic nephropathy by regulating NF-cB and apoptotic signaling pathways.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Integrative & Complementary Medicine
Su Wen-Ya, Fan Mei-Ling, Li Ying, Hu Jun-Nan, Cai En-Bo, Zhu Hong-Yan, Song Ming-Jie, Li Wei
Summary: This study found the protective effects of ginsenoside Rh1 (G-Rh1) on liver injury induced by type 2 diabetes (T2DM), including maintaining blood glucose and insulin levels, reducing triglyceride, total cholesterol and low-density lipoprotein cholesterol levels, and relieving apoptosis in liver tissue. Furthermore, G-Rh1 protected the liver by inhibiting gluconeogenesis pathway and inflammatory response, possibly through inhibiting the Akt/FoxO1 signaling pathway activation.
CHINESE JOURNAL OF NATURAL MEDICINES
(2022)
Article
Biochemistry & Molecular Biology
Wei Liu, Hao Zhang, Yun-yi Hou, Rui-yi Hu, Jun-jie Zhang, Xuan Chen, Shuang Wang, Jun-nan Hu, Zi Wang, Wei Li
Summary: This study evaluates the protective effects of arginyl-fructosyl-glucose (AFG), a major Maillard reaction product of red ginseng, against cisplatin-induced intestinal toxicity. The results showed that AFG can alleviate cisplatin-induced gastrointestinal damage and inhibit cell apoptosis by regulating the Wnt/beta-catenin signaling pathway.