Review
Biology
Kobi Simpson-Lavy, Martin Kupiec
Summary: Glucose is the preferred carbon source for most cells. Yeast Snf1 is the main regulator of the response to low glucose availability. Three different mechanisms, including phosphorylation, SUMOylation, and protonation, independently control Snf1 activity, allowing a flexible response to carbon source availability.
Article
Biochemical Research Methods
Luis Fernando Montano-Gutierrez, Kevin Correia, Peter S. Swain
Summary: This article discusses why cells express multiple different types of nutrient transporters and proposes a tradeoff between rate and affinity in cellular nutrient transport. By using mathematical modelling and experimental verification, the study shows that cells can mitigate the tradeoff by encoding multiple transporters and expressing them at the appropriate concentrations for optimal nutrient uptake.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Riccardo Milanesi, Farida Tripodi, Jacopo Vertemara, Renata Tisi, Paola Coccetti
Summary: This study reveals that glucose-dependent inactivation of Snf1/AMPK is unrelated to the Ras/PKA pathway, and demonstrates a strong coordination between glycolytic metabolism and Snf1/AMPK. The findings support a model in which Snf1/AMPK senses glucose flux independently from PKA activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Romain Laurian, Jade Ravent, Karine Dementhon, Marc Lemaire, Alexandre Soulard, Pascale Cotton
Summary: The survival of the pathogenic yeast Candida albicans relies on the assimilation of fermentable and non-fermentable carbon sources, particularly glucose, through the enzymatic and regulatory functions of hexokinase 2 (CaHxk2). The interaction with glucose, mediated by a conserved aspartate residue, is not only essential for enzymatic activities and glucose repression but also for filamentation and virulence in macrophages. Point mutations and deletions affecting glucose repression in Saccharomyces cerevisiae hexokinase 2 were found to be ineffective in CaHxk2, highlighting the interconnectedness of enzymatic and regulatory functions in C. albicans.
Article
Biochemical Research Methods
Luis Fernando Montano-Gutierrez, Nahuel Manzanaro Moreno, Iseabail L. Farquhar, Yu Huo, Lucia Bandiera, Peter S. Swain
Summary: omniplate is a Python module that allows for the analysis of plate reader data. It corrects and normalizes data, estimates growth rates and fluorescence, and enables meta-analysis of multiple plates. The authors demonstrate its effectiveness in revealing biological regulation mechanisms through experimental results.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Aroa Ferrer-Pinos, Victor Garrigos, Emilia Matallana, Agustin Aranda
Summary: Wine strains of Saccharomyces cerevisiae need to adapt their metabolism to different growth conditions during biotechnological applications, with the transcription factor Gln3 playing a crucial role in glucose and nitrogen repression. Commercial yeast strains exhibit a variety of glucose repression profiles, and deletion of the GLN3 gene results in different mutant phenotypes. The nitrogen catabolite repression controlled by TORC1 is largely conserved in winemaking conditions, with mutants showing increased tolerance to rapamycin inhibition of nutrient signaling complex TORC1.
FERMENTATION-BASEL
(2021)
Review
Biochemistry & Molecular Biology
Alessandra Ferramosca, Vincenzo Zara
Summary: Yeast mitochondrial carriers play a crucial role in cellular metabolism by connecting mitochondrial and cytosolic reactions, contributing to various important metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Jana Fees, Jonas J. Christ, Sabine Willbold, Lars M. Blank
Summary: A biotechnological process using Saccharomyces cerevisiae to upgrade phosphate-containing wastewater into pure sodium polyphosphate was developed in this study. The quality of polyphosphate was independent of the composition of the wash water and can be reused in various applications, contributing to the sustainable utilization of phosphate and reducing the need for phosphate rock.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Microbiology
Xu Yang, Lu Meng, Xue Lin, Huan-Yuan Jiang, Xiao-Ping Hu, Cong-Fa Li
Summary: Glucose repression plays a crucial role in controlling the metabolism of non-glucose carbon sources in yeast. The three kinases Elm1, Tos3, and Sak1 were investigated for their roles in maltose metabolism of baker's yeast in lean dough, with Elm1 identified as the dominant regulator.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Thomas B. Bailey, Phaedra A. Whitty, Eric U. Selker, Jeffrey N. McKnight, Laura E. McKnight
Summary: Upon glucose starvation, S. cerevisiae undergoes significant transcriptional changes, with most genes being repressed and some genes being activated. Tup1 is a critical factor for transcriptional repression after glucose depletion and is involved in the regulation of glucose metabolism and transport genes. Tup1 coordinates with other factors like Rpd3L and Isw2 to achieve transcriptional repression.
Article
Multidisciplinary Sciences
Piotr Hapeta, Patrycja Szczepanska, Cecile Neuveglise, Zbigniew Lazar
Summary: YlHxk1's loop region is essential for its activity and growth of Y. lipolytica on glucose and fructose. The loop hinders binding with T6P and affects lipase-encoding genes expression, as well as the preference for glycerol over glucose.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Hailong Chen, Xiaoqin Chai, Yan Wang, Jing Liu, Guohai Zhou, Pinghe Wei, Yuhe Song, Lingman Ma
Summary: Background: Saccharomyces cerevisiae is commonly used for the production of S-adenosyl-l-methionine (SAM), but glucose repression negatively affects its production. This study shows that deleting REG1 and overexpressing SNF1 can improve SAM production in S. cerevisiae by enhancing glucose utilization and glycolysis, promoting ethanol conversion, enhancing precursor amino acid biosynthesis, and extending yeast lifespan.
MICROBIAL CELL FACTORIES
(2022)
Article
Multidisciplinary Sciences
Thoma Itoh, Takashi Makino
Summary: Recent advancements in high throughput single cell RNA-seq have enabled the identification of functional compensation effects of gene perturbations through noise change, which can be measured quantitatively and captured in gene regulatory networks.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Mrinal Sharma, Prranshu Yadav, Ankita Doshi, Hemang D. Brahmbhatt, C. Ratna Prabha
Summary: The study examined the effects of six double mutants derived from the ubiquitin mutant UbEP42 on cell functions, finding that the double mutation L50P-I61T had the most detrimental impact. Overall, these double mutations made cells more sensitive, and the negative effects under certain conditions could be compensated for by each other.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Water Resources
Mohaddeseh Zahmatkesh Anbarani, Afsaneh Esmaeili Nasrabadi, Ziaeddin Bonyadi
Summary: The aim of this study was to evaluate the efficiency of S. cerevisiae in removing PS from aqueous solutions. BBD was used to determine the optimal removal conditions. The maximum PS removal efficiency was 98.81% under optimized conditions. Based on the results, it can be concluded that S. cerevisiae can be used as a natural and environmentally friendly biocoagulant to remove PS.
APPLIED WATER SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Diana Silva, Herminio C. de Sousa, Maria Helena Gil, Luis F. Santos, Madalena Salema Oom, Carmen Alvarez-Lorenzo, Benilde Saramago, Ana Paula Serro
Summary: This study developed a molecularly imprinted silicone-based hydrogel for the manufacturing of contact lenses that can efficiently load and sustainably release the antibiotic moxifloxacin hydrochloride (MXF). The modified hydrogel showed promising results in treating ocular infections, with no cytotoxicity or ocular irritancy effects detected.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
E. Massarelli, D. Silva, A. F. R. Pimenta, A. Fernandes, J. L. G. Mata, H. Armes, M. Salema-Oom, B. Saramago, A. P. Serro
Summary: A new PVA/Ch based wound dressing was developed, which showed protection, hydration, and controlled release of antiseptics. The dressings exhibited antibacterial activity against S. aureus and S. epidermidis, with the ones loaded with PHMB showing better cytotoxicity and irritability properties. The application of this dressing in wound treatment in a dog led to faster recovery compared to conventional treatment, suggesting its potential as an alternative in wound care.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Microbiology
Alexandra Cabrita, Marcia David-Palma, Patricia H. Brito, Joseph Heitman, Marco A. Coelho, Paula Goncalves
Summary: The genetic basis of homothallism in a fungal lineage rich in homothallic species, the Cystofilobasidiales, was found to be distinct from that of other related species. Homothallism in this lineage may involve constitutive activation or dispensability of the pheromone receptor and the functional replacement of the usual Hd1/Hd2 heterodimer by an Hd2 homodimer. This suggests that homothallism evolved independently multiple times within the Cystofilobasidiales, supporting the idea that transitions between heterothallism and homothallism are not as rare as previously thought.
Article
Biochemistry & Molecular Biology
Helena Laronha, Ines Carpinteiro, Jaime Portugal, Ana Azul, Mario Polido, Krasimira T. Petrova, Madalena Salema-Oom, Isabel Barahona, Jorge Caldeira
Summary: A new class of MMP inhibitors has been discovered, which can be co-polymerized with other vinyl compounds in commercial dental resins, limiting release and potential toxicity, and increasing safety. These inhibitors show unique properties towards various MMPs and did not significantly decrease cell viability at high concentrations.
Article
Pharmacology & Pharmacy
Nadia Toffoletto, Madalena Salema-Oom, Soledad Anguiano Igea, Carmen Alvarez-Lorenzo, Benilde Saramago, Ana Paula Serro
Summary: Pseudophakic cystoid macular edema (PCME), caused by chronic inflammation, is the most common cause of visual impairment in the medium-term after cataract surgery. Prophylactic treatment commonly involves the topical administration of combined steroidal and non-steroidal anti-inflammatory drugs. Drug-eluting intraocular lenses (IOLs) have shown to be an efficient way to deliver drugs, with increased drug absorption and improved release kinetics, which could maintain therapeutic drug concentrations in the aqueous humor for about 2 and 8 weeks.
Article
Biotechnology & Applied Microbiology
Bruno M. Pedras, Carla Goncalves, Diogo R. Figueira, Pedro Simoes, Paula Goncalves, Alexandre Paiva, Susana Barreiros, Madalena Salema-Oom
Summary: This study successfully utilized non-structural sugars from white wine grape pomace as the carbon source for the production of multiple high-value products by Rhodotorula babjevae, highlighting its high biotransformation capability and fructophilic character.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Environmental Sciences
Sara Lopes, Vitor Tavares, Paulo Mascarenhas, Marta Lopes, Carolina Cardote, Catarina Godinho, Catia Oliveira, Carla Adriana Santos, Madalena Oom, Jose Grillo-Evangelista, Jorge Fonseca
Summary: This study evaluated the oral health and changes of PEG patients without oral feeding for three months. The results showed that PEG patients had poor oral health and insufficient oral hygiene habits even before gastrostomy. After three months of PEG feeding, oral health deteriorated in general.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Microbiology
Carla Goncalves, Margarida Marques, Paula Goncalves
Summary: Microbes have flexible metabolic capabilities and can use different compounds to meet their needs. Yeasts belonging to the Wickerhamiella and Starmerella genera (W/S clade) are usually found in flowers or insects and have acquired many genes from bacteria. This study investigates sucrose utilization in the W/S clade and uncovers different strategies and modes of sucrose assimilation. This research provides insights into the ecological implications and energy metabolism of disaccharide utilization in these yeasts.
Article
Materials Science, Ceramics
N. Ribeiro, M. Reis, L. Figueiredo, A. Pimenta, L. F. Santos, A. C. Branco, A. P. Alves de Matos, M. Salema-Oom, A. Almeida, M. F. C. Pereira, R. Colaco, A. P. Serro
Summary: The study aimed to enhance the properties of commercial CPC for injectability and as a sustained antibiotic delivery system. The optimal formulation contained 42%LP + HPMC + 1.87% GS, displaying excellent properties and injectability, with sustained release of antibiotic for at least 14 days.
CERAMICS INTERNATIONAL
(2022)
Article
Cell Biology
Paula Goncalves, Carla Goncalves
Summary: Horizontal gene transfer (HGT) is a common genetic phenomenon in bacteria, but less common in eukaryotes. Recently, there have been numerous reports of HGT in yeasts based on strict criteria, with many involving filamentous fungi and yeasts. This article summarizes the most prominent instances of HGT reported in yeasts and provides an overview of research progress in terms of frequency, distribution, mechanisms, barriers, function of horizontally acquired genes, and the role of HGT in domestication.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2022)
Article
Pharmacology & Pharmacy
Leonor Vasconcelos Garcia, Diana Silva, Maria Madalena Costa, Henrique Armes, Madalena Salema-Oom, Benilde Saramago, Ana Paula Serro
Summary: Chronic wound treatment is a major contributor to global medical expenses. The development of novel wound care systems, such as wound dressings loaded with antiseptics, can offer a solution. This study investigated the potential of casein hydrogel dressings loaded with Octiset(R) or polyhexanide for treating chronic wounds. The hydrogels showed desirable physical properties and sustained controlled drug-release, while also exhibiting good antimicrobial properties and compatibility with biological systems.
Article
Multidisciplinary Sciences
David Peris, Emily J. Ubbelohde, Meihua Christina Kuang, Jacek Kominek, Quinn K. Langdon, Marie Adams, Justin A. Koshalek, Amanda Beth Hulfachor, Dana A. Opulente, David J. Hall, Katie Hyma, Justin C. Fay, Jean-Baptiste Leducq, Guillaume Charron, Christian R. Landry, Diego Libkind, Carla Goncalves, Paula Goncalves, Jose Paulo Sampaio, Qi-Ming Wang, Feng-Yan Bai, Russel L. Wrobel, Chris Todd Hittinger
Summary: The authors describe the geographical distribution, hosts, substrates, and phylogenetic relationships for 1,794 Saccharomyces strains. Through analyzing complete genome sequences of 163 strains and phenotyping 128 phylogenetically diverse strains, they provide insights into how gene flow and selection have affected traits in the evolution of this genus. These findings elevate the importance of Saccharomyces as a model for understanding biodiversity and evolution in microbial eukaryotes.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Carlos A. Rosa, Marc-Andre Lachance, Savitree Limtong, Ana R. O. Santos, Mellisa F. Landell, Andreas K. Gombert, Paula B. Morais, Jose P. Sampaio, Carla Goncalves, Paula Goncalves, Aristoteles Goes-Neto, Rosangela Santa-Brigida, Marlucia B. Martins, Daniel H. Janzen, Winnie Hallwachs
Summary: Tropical rainforests and related biomes are tremendous sources of novel yeasts. Tropical yeasts participate in several ecological interactions that contribute to the health of these ecosystems. Most studies of yeast diversity in tropical forests were carried out using culture-dependent methods. Yeasts from tropical rainforests are rich sources for bioinnovation.
Article
Polymer Science
Tomas Pires, Andreia Sofia Oliveira, Ana Clara Marques, Madalena Salema-Oom, Celio G. Figueiredo-Pina, Diana Silva, Ana Paula Serro
Summary: In this study, a non-conventional sterilisation method, microwave, was found to be the most suitable for polyvinyl alcohol (PVA) hydrogel reinforced with poly(p-phenylene-2,6-benzobisoxazole) (PBO) nanofibres. The microwave sterilised PVA hydrogel showed improved mechanical properties and biocompatibility.