Journal
ACTA BIOMATERIALIA
Volume 20, Issue -, Pages 69-81Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2015.03.032
Keywords
Polycarbonate urethane; Peptide; Endothelialization; Anti-thrombogenic surface; Click chemistry
Funding
- International Cooperation from Ministry of Science and Technology of China [2008DFA51170, 2013DFG52040]
- National Natural Science Foundation of China [31370969]
- Ph.D. Programs Foundation of Ministry of Education of China [20120032110073]
- Program of Introducing Talents of Discipline to Universities of China [B06006]
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Surface tailoring is an attractive approach to enhancing selective endothelialization, which is a prerequisite for current vascular prosthesis applications. Here, we modified polycarbonate urethane (PCU) surface with both poly(ethylene glycol) and Cys-Ala-Gly-peptide (CAG) for the purpose of creating a hydrophilic surface with targeting adhesion of endothelial cells (ECs). In the first step, PCU-film surface was grafted with poly(ethylene glycol) methacrylate (PEGMA) to covalently tether hydrophilic polymer brushes via surface initiated atom transfer radical polymerization (SI-ATRP), followed by grafting of an active monomer pentafluorophenyl methacrylate (PFMA) by a second ATRP. The post-polymerization modification of the terminal reactive groups with allyl amine molecules created pendant allyl groups, which were subsequently functionalized with cysteine terminated CAG-peptide via photo-initiated thiol-ene click chemistry. The functionalized surfaces were characterized by water contact angle and XPS analysis. The growth and proliferation of human ECs or human umbilical arterial smooth muscle cells on the functionalized surfaces were investigated for 1, 3 and 7 day/s. The results indicated that these peptide functionalized surfaces exhibited enhanced EC adhesion, growth and proliferation. Furthermore, they suppressed platelet adhesion in contact with platelet-rich plasma for 2 h. Therefore, these surfaces with EC targeting ligand could be an effective anti-thrombogenic platform for vascular tissue engineering application. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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