Article
Multidisciplinary Sciences
Doreen Becker, Rosemarie Weikard, Frieder Hadlich, Christa Kuehn
Summary: The analysis of single-cell RNA datasets from bovine milk cells and pbMECs revealed distinct clusters within each population, indicating heterogeneity between the populations. These datasets provide a molecular cell atlas for future studies of milk cell composition and gene expression.
Article
Cell Biology
Taylor R. Rutherford, Alan M. Elder, Traci R. Lyons
Summary: The study reveals that SEMA7A promotes anoikis resistance in mammary epithelial cells and plays a role during mammary gland involution. SEMA7A is primarily expressed in cells expressing alpha 6-integrin, and a decrease in luminal progenitor cells is observed in SEMA7A-/- mice during involution.
CELL DEATH & DISEASE
(2021)
Article
Cell & Tissue Engineering
Junichi Matsuo, Naing Naing Mon, Daisuke Douchi, Akihiro Yamamura, Madhura Kulkarni, Dede Liana Heng, Sabirah Chen, Napat Nuttonmanit, Ying Li, Henry Yang, May Yin Lee, Wai Leong Tam, Motomi Osato, Linda Shyue Huey Chuang, Yoshiaki Ito
Summary: Researchers have identified an active element called eR1 that can mark mammary stem cells and found its activity in a small population of luminal epithelial cells in the mammary glands. They demonstrated that eR1+ luminal cells can generate the entire luminal lineage and milk-secreting alveoli, indicating that eR1 specifically marks lineage-restricted luminal stem cells.
Article
Nanoscience & Nanotechnology
Apoorva S. S. Metkari, Eric W. W. Fowler, Robert L. L. Witt, Xinqiao Jia
Summary: Synthetic matrices with varying degrees of degradability were synthesized to engineer implantable salivary gland constructs. The degradable matrices promoted cell proliferation and development of multicellular structures with a secretary phenotype, as evidenced by the upregulation of acinar markers. Cell-mediated matrix remodeling was found to be necessary for the development of regenerative pro-acinar progenitor cells.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Agriculture, Dairy & Animal Science
Yutaka Suzuki, Sachi Chiba, Koki Nishihara, Keiichi Nakajima, Akihiko Hagino, Won-Seob Kim, Hong-Gu Lee, Tomonori Nochi, Toru Suzuki, Sang-Gun Roh
Summary: This study explored the role of chemerin in promoting bovine mammary epithelial cell growth and protecting against oxidative stress, suggesting its potential as a therapeutic target for diseases in the bovine mammary gland.
Article
Multidisciplinary Sciences
Ruiqi Liu, Huan Hu, Melissa McNeil, Jiuzhi Xu, Xueyun Bi, Pengbo Lou, Christian F. Guerrero-Juarez, Xing Dai, Maksim Plikus, Jianwei Shuai, Zhengquan Yu, Cong Lv
Summary: During pregnancy, Nfatc1 reporter-marked basal epithelial cells, which are dormant mammary stem/progenitor cells, play an important role in the development of mammary lobuloalveoli.
Article
Biochemical Research Methods
Dailin Gan, Jun Li
Summary: SCIBER is a new batch-effect-removal method that can remove batch effects before integrating multiple single-cell RNA-sequencing datasets to avoid misleading interpretations. It has better scalability and tunability compared to existing methods, and it can output expression data of individual genes for downstream analyses.
Article
Cell Biology
Hui Gao, Yahui Liu, Min Zheng, Fan Zhao, Hongzhu Wang, Jiajian Yu, Hao Jiang, Danhan Wang, Qiaoxiang Dong
Summary: The study found that in the D-galactose-induced accelerated aging model, mammary stem/progenitor cells exhibit similar pathological changes and functions to those in mammary glands of naturally aged mice. This model can be used as a surrogate to study the role aged stem/progenitor cells play in breast tumorigenesis.
Review
Cell Biology
Christine J. Watson
Summary: Research on mammary stem cells is currently facing controversies, but with innovative experimental approaches and technologies, there is a reevaluation of the concept of stem and progenitor cell hierarchy in progress.
TRENDS IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rong Xuan, Jianmin Wang, Qing Li, Yanyan Wang, Shanfeng Du, Qingling Duan, Yanfei Guo, Peipei He, Zhibin Ji, Tianle Chao
Summary: This study conducted transcriptome sequencing of goat-mammary-gland tissue at different developmental stages to reveal the expression characteristics and molecular functions of circRNAs during mammary involution. A total of 11,756 circRNAs were identified, with 2528 circRNAs expressed in all three stages. Gene ontology analysis showed functional diversity of circRNAs' source genes. Furthermore, differentially expressed circRNAs and circRNA-miRNA-mRNA ceRNA regulatory networks were identified, providing insights into the regulatory role of circRNAs in mammary cell involution and remodeling.
Article
Multidisciplinary Sciences
Holly Holliday, Daniel Roden, Simon Junankar, Sunny Z. Wu, Laura A. Baker, Christoph Krisp, Chia-Ling Chan, Andrea McFarland, Joanna N. Skhinas, Thomas R. Cox, Bhupinder Pal, Nicholas D. Huntington, Christopher J. Ormandy, Jason S. Carroll, Jane Visvader, Mark P. Molloy, Alexander Swarbrick
Summary: ID4 functions as a repressor of myoepithelial differentiation in mammary basal cells by regulating gene expression, antagonizing HEB transcriptional activity, and promoting appropriate tissue morphogenesis. During pregnancy, downregulation of ID4 modulates genes regulated by HEB, promoting specialization of basal cells into myoepithelial cells.
Article
Biochemistry & Molecular Biology
John Maringa Githaka, Leila Pirayeshfard, Ing Swie Goping
Summary: Cancer invasion and metastasis are the main causes of cancer-related mortality. Understanding the factors that drive the abnormal invasion and migration of tumor cells will help identify critical targets for inhibiting metastasis. The development of postnatal pubertal mammary glands, characterized by proliferative, invasive, and migratory normal epithelial cells, can provide insights into tumorigenesis and metastasis. This review summarizes the regulators of pubertal gland development that are involved in cancer metastasis and explores potential novel metastatic drivers.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2023)
Article
Cell Biology
Amritha Varshini Hanasoge Somasundara, Matthew A. Moss, Mary J. Feigman, Chen Chen, Samantha L. Cyrill, Michael F. Ciccone, Marygrace C. Trousdell, Macy Vollbrecht, Siran Li, Jude Kendall, Semir Beyaz, John E. Wilkinson, Camila O. dos Santos
Summary: Pregnancy induces expansion of NKT-like immune cells and upregulation of immune signaling molecules in mammary epithelial cells. Lack of CD1d expression or activated NKT cells may lead to mammary oncogenesis.
Article
Cell Biology
Jessica Elswood, Scott J. Pearson, H. Ross Payne, Rola Barhoumi, Monique Rijnkels, Weston W. Porter
Summary: Through studying the differentiation and metabolism of mammary epithelial cells, it was found that mitophagy occurs selectively during the differentiation process. Inhibiting autophagy impairs cell differentiation, while the PRKN protein is necessary for cell differentiation.
Article
Agriculture, Dairy & Animal Science
Holly R. Williamson, Nicola M. Schreurs, Stephen T. Morris, Rebecca E. Hickson
Summary: The study investigated the effect of dam breed on the growth and carcass characteristics of beef-cross-dairy-breed progeny. Results showed that dam breed had a significant impact on the growth traits and carcass characteristics of the calves, with an increase in Jersey genetics resulting in slower growth, lighter carcass, and yellower fat.
Review
Cardiac & Cardiovascular Systems
Daniel Jakubik, Alex Fitas, Ceren Eyileten, Joanna Jarosz-Popek, Anna Nowak, Pamela Czajka, Zofia Wicik, Harald Sourij, Jolanta M. Siller-Matula, Salvatore De Rosa, Marek Postula
Summary: Research suggests that miRNAs and lncRNAs play a crucial role in the development of diabetic cardiomyopathy, serving as potential biomarkers for diagnosis, prognosis, and therapeutic strategies.
CARDIOVASCULAR DIABETOLOGY
(2021)
Article
Physiology
Ceren Eyileten, Zofia Wicik, Alex Fitas, Mikolaj Marszalek, Jenny E. Simon, Salvatore De Rosa, Szczepan Wiecha, Jeffrey Palatini, Marek Postula, Lukasz A. Malek
Summary: The study evaluated the expression of miRNAs in ultramarathon runners before and after the race, showing significant increases in miR-125a-5p, miR-126, and miR-223 post-race. MiR-1-3p showed a negative correlation with hs-CRP levels post-race and was associated with faster race completion times.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Maciej Blaszczyk, Malgorzata Gajewska, Marta Dymowska, Alicja Majewska, Tomasz Domoradzki, Adam Prostek, Rafal Pingwara, Magdalena Hulanicka, Katarzyna Grzelkowska-Kowalczyk
Summary: IL-6 modulates the distribution of certain proteins in adipocytes and mimics the effect of insulin on cellular accumulation of specific molecules. However, IL-6 does not affect insulin action on actin distribution.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Neurosciences
Anna Nowak, Zofia Wicik, Marta Wolska, Andleeb Shahzadi, Piotr Szwed, Joanna Jarosz-Popek, Jeffrey Palatini, Marek Postula, Anna Czlonkowska, Dagmara Mirowska-Guzel, Ceren Eyileten
Summary: This article reviews the latest research on ncRNAs in MS, evaluates their role in neuroinflammation, and highlights the most promising ncRNAs that may serve as diagnostic and prognostic biomarkers for MS.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Zofia Wicik, Pamela Czajka, Ceren Eyileten, Alex Fitas, Marta Wolska, Daniel Jakubik, Dirk von Lewinski, Harald Sourij, Jolanta M. Siller-Matula, Marek Postula
Summary: MicroRNAs (miRNAs) are small, non-coding RNAs that can regulate cellular functions by inducing mRNA degradation and post-transcriptional repression of gene expression. Platelets are a major source of circulating miRNAs and have significant regulatory potential in cardiovascular pathophysiology and other diseases. Circulating miRNAs can be used as diagnostic and prognostic biomarkers, as well as therapeutic targets, for cardiovascular diseases and other platelet-related diseases.
Article
Cardiac & Cardiovascular Systems
Ceren Eyileten, Zofia Wicik, Disha Keshwani, Faisal Aziz, Felix Aberer, Peter N. Pferschy, Norbert J. Tripolt, Caren Sourij, Barbara Prietl, Florian Pruller, Dirk von Lewinski, Salvatore De Rosa, Jolanta M. Siller-Matula, Marek Postula, Harald Sourij
Summary: Hypoglycemia was found to influence the expression of miRNAs related to diabetes and platelet activation. These findings suggest that miRNAs could potentially serve as biomarkers for platelet activation in patients with diabetes.
CARDIOVASCULAR DIABETOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ceren Eyileten, Zofia Wicik, Sergio N. Simoes, David C. Martins-Jr, Krzysztof Klos, Wojciech Wlodarczyk, Alice Assinger, Dariusz Soldacki, Andrzej Chcialowski, Jolanta M. Siller-Matula, Marek Postula
Summary: By utilizing bioinformatic and co-expression analysis, this study identified and validated miRNAs associated with thrombosis in COVID-19 patients, which can serve as potential diagnostic and prognostic biomarkers for disease severity. The findings contribute to our understanding of the pathogenesis of COVID-19 and the identification of novel predictive markers.
Article
Pharmacology & Pharmacy
Zofia Wicik, Anna Nowak, Joanna Jarosz-Popek, Marta Wolska, Ceren Eyileten, Jolanta M. M. Siller-Matula, Dirk von Lewinski, Harald Sourij, Krzysztof J. J. Filipiak, Marek Postula
Summary: This study provides comprehensive and ranked information about the SGLT2 interaction network in the context of tissue expression and can help to predict the clinical effects of the SGLT2i.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Alicja Majewska, Kourou Dembele, Katarzyna Dziendzikowska, Adam Prostek, Malgorzata Gajewska
Summary: This study observed the effect of allergen-specific immunotherapy on canine atopic dermatitis patients. The results showed that immunotherapy can reduce cytokine levels, alter the numbers of specific T cell subpopulations, and have an impact on regulatory T cells.
Article
Agriculture, Dairy & Animal Science
Christine Leroux, Karol Pawlowski, Pierre-Alexis Billa, Jose A. A. Pires, Yannick Faulconnier
Summary: This study aimed to validate the use of milk fat globules (MFGs) as a source of small non-coding RNAs (miRNAs) for diagnosing mastitis. By comparing the abundance of six miRNAs known to be regulated during inflammation in MFGs from cows before and after lipopolysaccharide (LPS) challenge, it was found that four miRNAs were differentially abundant according to inflammatory status. Bioinformatics analyses predicted that these miRNAs target genes involved in cell life regulation and gene expression. Network analyses revealed their association with the interleukin signaling network.
Article
Neurosciences
Natalia Chmielewska, Adriana Wawer, Zofia Wicik, Bartosz Osuch, Piotr Maciejak, Janusz Szyndler
Summary: The lack of understanding of the mechanisms of refractory epilepsy is the main obstacle in developing new treatment strategies. This study evaluated the expression of sodium channel subunits and miRNAs in lamotrigine-resistant seizures and found that miR-9a-5p was significantly associated with resistance to lamotrigine.
Article
Medicine, Research & Experimental
Michael J. Emch, Zofia Wicik, Kirsten G. M. Aspros, Tanja Vukajlovic, Kevin S. Pitel, Anders K. Narum, Megan M. Weivoda, Xiaojia Tang, Krishna R. Kalari, Russell T. Turner, Urszula T. Iwaniec, David G. Monroe, Malayannan Subramaniam, John R. Hawse
Summary: Estrogen signaling is crucial for bone development and maintenance, and decreased estrogen levels contribute to post-menopausal osteoporosis. This study investigated the transcriptomic differences between cortical and trabecular bones in response to hormonal changes using a mouse model. Seven miRs were identified as potential contributors to estrogen-mediated mRNA expression changes, and four miRs showed therapeutic potential in preventing bone loss without the side effects of hormone replacement therapy.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biochemistry & Molecular Biology
Alicja Majewska, Malgorzata Gajewska, Kourou Dembele
Summary: Canine atopic dermatitis (cAD) is a chronic and recurrent inflammatory skin disorder. Allergen-specific immunotherapy (ASIT) is recognized as an effective treatment for allergies. This study analyzed changes in gene expression in cAD patients after undergoing ASIT. Nine differentially expressed genes related to the dysregulated immune response in cAD patients were identified, and the expressions of five of these genes changed after ASIT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Zaneta Dziegelewska-Sokolowska, Alicja Majewska, Adam Prostek, Malgorzata Gajewska
Summary: The mammary gland consists of epithelial tissue and stroma, with preadipocytes and adipocytes as important components of the stroma. This study investigated the effects of paracrine factors secreted by preadipocytes and adipocytes on the development of bovine mammary epithelial cells. The results showed that factors secreted by fully differentiated adipocytes and preadipocytes can decrease apoptosis and increase the size of spheroids formed by the mammary epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
