Journal
JOURNAL OF APPLIED GENETICS
Volume 51, Issue 2, Pages 169-183Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/BF03195725
Keywords
canine mammary cancer cells; DNA microarray; transcriptomics
Funding
- Polish Ministry of Science and Higher Education [N308230536]
- European Social Fund
- Masovia Region
- Lower Silesia Region
Ask authors/readers for more resources
In light of the high incidence of mammary cancer in dogs and completion of the canine genome sequencing, the new possibilities of gene profiling by using DNA microarrays give hope to veterinary oncology. The cell lines isolated from mammary tumors are a valuable tool in developing and testing new pathway-specific cancer therapeutics. Differential cytometric analysis of 6 canine mammary cancer cell lines was performed. We divided cell lines into 3 groups based on their phenotype: 2 lines with high proliferative potential, 2 lines with high antiapoptotic potential, and 2 lines with high metastatic potential. DNA microarray analysis revealed common genes for cell lines of each group. We found that genes encoding the receptors for growth hormone and ghrelin are related to high proliferation rate, while A BR (active BCR-related) and TMD1 (TM2 domain containing 1) genes are related to a high antiapoptotic potential of the cancer cells. Metastatic properties of mammary cancer cells seem to be associated with elevated expression of PGP (P glycoprotein), SEMA3B (semaphorin 3B), and (stromal interaction molecule 1).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available