Journal
JOURNAL OF APPLIED GENETICS
Volume 51, Issue 1, Pages 9-18Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/BF03195705
Keywords
Aegilops longissima; DNA marker; LMW-GS gene; S-1 genome; Sitopsis
Funding
- Science and Technology Foundation of Dujiangyan Campus, Sichuan Agricultural University [200701]
- Sichuan Provincial Education Department [08ZA068]
- Key Technologies R&D Program of China [2006BAD01A02-23, 2006BAD13B02]
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Extensive genetic variations of low-molecular-weight glutenin subunits (LMW-GS) and their coding genes were found in the wild diploid A- and D-genome donors of common wheat. In this study, we reported the isolation and characterization of 8 novel LMW-GS genes from Ae.longissima Schweinf. & Muschl., a species of the section Sitopsis of the genus Aegilops, which is closely related to the B genonic of common wheat. Based on the N-terminal domain sequences, the 8 genes were divided into 3 groups. A consensus alignment of the extremely conserved domains with known gene groups and the subsequent cluster analysis showed that 2 out of the 3 groups of LMW-GS genes were closely related to those from the B genonic, and the remaining was related to those from A and D genomes of wheat and Ae. tauschii. Using 3 sets of gene-group-specific primers, PCRs in diploid, tetraploid and hexaploid wheats and Ae. tauschii failed to obtain the expected products, indicating that file 3 groups of LMW-GS genes obtained in this study were new members of LMW-GS multi-gene families. These results suggested that the Sitopsis species of the genus Aegilops with novel gene variations could be used as valuable gene resources of LMW-GS. The 3 sets of group-specific primers could be utilized as molecular markers to investigate the introgression of novel alien LMW-GS genes from Ae. longissima into wheat.
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