Article
Nutrition & Dietetics
Luigi Barrea, Giovanna Muscogiuri, Gabriella Pugliese, Chiara Graziadio, Maria Maisto, Francesca Pivari, Andrea Falco, Gian Carlo Tenore, Annamaria Colao, Silvia Savastano
Summary: Individual differences in chronotype are associated with cardiometabolic risk and gut dysbiosis. Morning chronotype is linked to lower BMI, better metabolic profile, higher adherence to the Mediterranean diet, and lower circulating TMAO concentrations. Higher chronotype scores are mainly associated with lower TMAO concentrations.
Review
Physiology
Katongo H. Mutengo, Sepiso K. Masenga, Aggrey Mweemba, Wilbroad Mutale, Annet Kirabo
Summary: The human gut microbiota and its dependant metabolite, trimethylamine N-oxide, play significant roles in the development of hypertension. This review examines the impact of the gut microbiota and trimethylamine N-oxide on hypertension pathogenesis, providing evidence from both human and animal studies. New insights into potential therapies for managing hypertension by altering the gut microbiota are also discussed.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Nutrition & Dietetics
Mauro Lombardo, Giovanni Aulisa, Daniele Marcon, Gianluca Rizzo, Maria Grazia Tarsisano, Laura Di Renzo, Massimo Federici, Massimiliano Caprio, Antonino De Lorenzo
Summary: There is a strong correlation between TMAO and fish consumption, particularly saltwater fish and shellfish, while the associations of TMAO with eggs, dairy, and meat are less clear. Plant-based foods do not seem to influence TMAO levels significantly, but this area requires further investigation.
Article
Nutrition & Dietetics
Jose L. Flores-Guerrero, Maryse C. J. Oste, Paula B. Baraldi, Margery A. Connelly, Erwin Garcia, Gerjan Navis, Stephan J. L. Bakker, Robin P. F. Dullaart
Summary: This study revealed that trimethylamine-N-oxide (TMAO) and its dietary determinants are associated with an increased risk of graft failure in renal transplant recipients (RTRs). A predictive model enriched with TMAO and dietary factors showed a higher Net Benefit compared to a previously validated model.
Article
Immunology
Qiqi Wang, Yue Sun, Tianyu Zhou, Cong Jiang, A. Lan, Wenzhou Xu
Summary: This study found that intestinal inflammation and periodontitis influence each other through a bidirectional relationship. The intestinal microbiome metabolite, TMAO, may contribute to chronic inflammation in the gut by influencing the gut microbial composition and intestinal immunity. The results suggest that TMAO may influence periodontal immunity and promote periodontal inflammation by affecting the intestinal microenvironment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Nutrition & Dietetics
Edoardo Tacconi, Giuseppe Palma, Davide De Biase, Antonio Luciano, Massimiliano Barbieri, Filomena de Nigris, Francesca Bruzzese
Summary: TMAO is a microbial metabolite that is closely related to gut microbiota and has implications for gastrointestinal cancers, cardiovascular disease, and systemic inflammation. The composition of gut microbiota plays a crucial role in the production of pro-inflammatory TMAO, and the culprits may be certain food sources such as high fat meat, offal, egg yolk, whole dairy products, and fatty fish.
Article
Gastroenterology & Hepatology
Jose L. Flores-Guerrero, Adrian Post, Peter R. van Dijk, Margery A. Connelly, Erwin Garcia, Gerjan Navis, Stephan J. L. Bakker, Robin P. F. Dullaart
Summary: This study revealed that plasma concentrations of TMAO were associated with all-cause mortality in subjects with NAFLD, independently of traditional risk factors. However, such an association was not present in subjects without NAFLD.
LIVER INTERNATIONAL
(2021)
Review
Nutrition & Dietetics
Lin Zhang, Fang Yu, Jian Xia
Summary: TMAO is closely associated with cell senescence and age-related diseases, and it can accelerate aging through multiple pathways.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Crystallography
Ki Hyun Nam
Summary: Cryocrystallography is a widely used method for determining the crystal structure of macromolecules. This study introduces a method for processing multicrystal diffraction data using a serial crystallography program, which allows for the integration of diffraction patterns from multiple crystals in a single image.
Article
Biochemistry & Molecular Biology
Giulia Querio, Susanna Antoniotti, Federica Geddo, Renzo Levi, Maria Pia Gallo
Summary: TMAO may be involved in impairing the endothelial-dependent vasodilatory mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Kristen L. James, Erik R. Gertz, Eduardo Cervantes, Ellen L. Bonnel, Charles B. Stephensen, Mary E. Kable, Brian J. Bennett
Summary: The study found that in metabolically healthy adults, TMAO levels were not associated with the intake of animal protein foods, fruits, vegetables, dairy, or grains, but were related to the fecal microbiome. TMAO was not associated with classic cardiovascular risk factors, but was related to endothelial function and the inflammatory marker TNF-α. More prospective studies in healthy individuals are needed.
Article
Chemistry, Analytical
Yu-Chun Chang, Yi-Hsuan Chu, Chien-Cheng Wang, Chih-Hsuan Wang, You-Lin Tain, Hung-Wei Yang
Summary: The gut microbiota-derived metabolites TMAO and TMA play a critical role in CKD and hypertension. A new color-switch method was developed for the direct quantitative detection of TMAO in whole blood, showing potential clinical applications in blood TMAO monitoring for CKD patients.
Article
Nutrition & Dietetics
Seok Jong Chung, John Hoon Rim, Dajeong Ji, Sangwon Lee, Han Soo Yoo, Jin Ho Jung, KyoungWon Baik, Yonghoon Choi, Byoung Seok Ye, Young H. Sohn, Mijin Yun, Sang-Guk Lee, Phil Hyu Lee
Summary: This study found that lower baseline plasma TMAO levels are associated with faster increases in LED and tend to increase the risk for PD-dementia conversion, suggesting the prognostic implications of TMAO in early stage PD.
Review
Biology
Paulina Gatarek, Joanna Kaluzna-Czaplinska
Summary: This review summarizes the effects of TMAO on human health, the relationship between TMAO and intestinal microbiota, as well as the role of TMAO in different diseases.
Article
Medicine, General & Internal
Jose L. Flores-Guerrero, Peter R. van Dijk, Margery A. Connelly, Erwin Garcia, Henk J. G. Bilo, Gerjan Navis, Stephan J. L. Bakker, Robin P. F. Dullaart
Summary: Research showed that high plasma TMAO concentrations in patients with T2D are associated with an increased risk of cardiovascular mortality, while BCAA concentrations are not.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Erin Day, Brice Kauffmann, Matthieu Scarpi-Luttenauer, Alain Chaumont, Marc Henry, Pierre Mobian
Summary: The formation of a novel tetranuclear self-assembled species constructed around a TiO4N2 motif is reported, and its structure and stability were investigated using DFT calculations. It was found that the exclusive formation of an unprecedented chiral alternate [2x2] grid is driven by CH... interactions.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Applied
Mathilde Bayard, Brice Kauffmann, Jean-Michael Vauvre, Fernando Leal-Calderon, Maud Cansell
Summary: This study employed a multiscale approach to investigate the impact of low concentration pure exogenous compounds on the crystallization of TAGs in AMF. The results showed that the presence of additives did not alter the nanostructure of TAG crystals, but significantly changed the microstructure of AMF.
Article
Biochemical Research Methods
Frank Lennartz, Jae Hun Jeoung, Stefan Ruenger, Holger Dobbek, Manfred S. Weiss
Summary: Protein-mediated redox reactions are crucial in biological processes, often occurring at metal ion centers. Spatially resolved anomalous dispersion (SpReAD) refinement is a suitable method for characterizing the oxidation states of cofactors and their structures. In this study, SpReAD analysis was applied to a ruberythrin-like protein, revealing differences in oxidation states of individual iron ions in a binuclear metal center.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2022)
Article
Agricultural Engineering
Estelle Morvan, Brice Kauffmann, Axelle Grelard, Antoine Loquet, Anne-Julie Dixon, Erick J. Dufourc, Denis Rontein
Summary: This study reports a new eco-responsible method to produce (E)-(R)-4-thujanol crystals on a kilogram scale, which may open up new opportunities for the flavor, fragrance and pharmaceutical industries.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Manfred S. Weiss, Jan Wollenhaupt, Galen J. Correy, James S. Fraser, Andreas Heine, Gerhard Klebe, Tobias Krojer, Marjolein Thunnissen, Nicholas M. Pearce
Summary: Jaskolski et al. analyzed diffraction data sets from fragment-screening group depositions and claimed that these data are problematic. However, we demonstrate that none of the criticisms persist if the data are treated properly.
Article
Chemistry, Medicinal
Tatjana Barthel, Jan Wollenhaupt, Gustavo M. A. Lima, Markus C. Wahl, Manfred S. Weiss
Summary: The identification of starting points for compound development is crucial in early-stage drug discovery. Crystallographic fragment screening provides structural information of the binding mode, increasing the efficiency of this step. In this study, crystallographic screening of a compound library was conducted, resulting in the identification of 269 hits distributed over 10 distinct binding sites on the protein-protein complex surface. The study demonstrates that hit clusters can identify known interaction sites and suggest potential additional interaction sites, thereby accelerating downstream compound optimization.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Organic
Friedericke S. S. Menke, Daniela Mazzier, Barbara Wicher, Lars Allmendinger, Brice Kauffmann, Victor Maurizot, Ivan Huc
Summary: The first abiotic foldamer tertiary structures have been reported, consisting of aromatic helix-turn-helix motifs based on oligo-quinolinecarboxamides held together by intramolecular hydrogen bonds. Computational modelling predicted and X-ray crystallography verified the tertiary folds. Investigation into the conformational preference of each helix revealed that the removal of some hydrogen bonds did not strongly destabilize the tertiary folds.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Thierry Mourer, Mennat El Ghalid, Gerard Pehau-Arnaudet, Brice Kauffmann, Antoine Loquet, Sebastien Brule, Vitor Cabral, Christophe d'Enfert, Sophie Bachellier-Bassi
Summary: The human commensal fungus Candida albicans can form biofilms that are highly tolerant to antifungal drugs and can evade the immune response. The cell surface protein Pga59 influences adhesion and biofilm formation and displays amyloid properties. Mutations impairing Pga59 amyloid assembly result in diminished adhesion and reduced biofilm production. Pga59 is used by the fungal cell to assemble amyloids within the cell wall in response to adhesion.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Chemistry, Multidisciplinary
Nagendar Pendem, Yella-Reddy Nelli, Leonie Cussol, Claude Didierjean, Brice Kauffmann, Christel Dolain, Gilles Guichard
Summary: Oligomers with a helix-turn-helix super-secondary structure were prepared by covalently bridging aliphatic oligourea foldamer helices with either rigid aromatic or flexible aliphatic spacers. The relative orientation of the helices in these dimers was studied using high-resolution X-ray diffraction analysis. Racemic crystallography was employed in some cases to facilitate crystallization and structure determination. All structures were solved using direct methods. Well-defined parallel helical hairpin motifs were observed in all cases when 4,4'-methylene diphenyl diisocyanate was used as a dimerizing agent, regardless of the primary sequence and chain length.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemical Research Methods
Friederike T. Fuesser, Jan Wollenhaupt, Manfred S. Weiss, Daniel Kuemmel, Oliver Koch
Summary: With the increasing number of tuberculosis-related deaths and the presence of drug-resistant strains, there is an urgent need for alternative antituberculotic drugs. In this study, crystallographic fragment screening using crystals of Mycobacterium smegmatis TrxR was used to discover new binding fragments and binding sites. The results identified 56 starting points for the development of new TrxR inhibitors.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2023)
Article
Chemistry, Physical
Johal Ruiz, Josephine G. LoRicco, Laurent Soulere, Marta Salvador Castell, Axelle Grelard, Brice Kauffmann, Erick J. Dufourc, Bruno Deme, Florence Popowycz, Judith Peters
Summary: Archaeal membrane lipids have unique structures that enable them to withstand extreme temperature and pressure conditions. A study on the synthesis of an archaeal lipid called 1,2-di-O-phytanyl-sn-glycero-3-phosphoinositol (DoPhPI) derived from myo-inositol reveals its resistance mechanisms. The experiments show that DoPhPI and DoPhPC can form small vesicles in aqueous dispersions, and these dispersions undergo phase transitions from lamellar to cubic and hexagonal phases with increasing temperature. The phytanyl chains in the lipid also contribute to the bilayer's dynamic properties over a wide temperature range. These findings suggest that the unique properties of archaeal lipids provide plasticity and aid in resisting extreme conditions.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Yulong Zhong, Thomas A. Sobiech, Brice Kauffmann, Bo Song, Xiaopeng Li, Yann Ferrand, Ivan Huc, Bing Gong
Summary: Two aromatic oligoamides, H8 and H16, were found to wrap around octyl viologen (OV2+) and para-bis(trimethylammonium)benzene (TB2+) to form 2:2 and 1:2 complexes, respectively. The longer H16 exhibited higher binding affinity with OV2+ and showed negative cooperativity. Interestingly, H16 selectively bound OV2+ over TB2+. This study highlights the pairwise placement of OV2+ ions, negative cooperativity, and the adaptability of hosts and guests, resulting in stable [2]-, [3]-, and [4]pseudo-foldaxanes.
Article
Chemistry, Multidisciplinary
Aline Delamare, Guillaume Naulet, Brice Kauffmann, Gilles Guichard, Guillaume Compain
Summary: The hexafluoroisobutyl group is a commonly found hydrophobic group in drugs, and its introduction can provide new advantages to compounds. However, the lack of a general and practical synthetic methodology has hindered the exploration of this modification. In this study, the first general method for introducing the hexafluoroisobutyl group into various compounds was reported. This method overcomes the usual fluoride beta-elimination observed with other CF3-vinyl groups, and it has been successfully applied to the multigram-scale synthesis of enantiopure (S)-5,5,5,5',5',5'-hexafluoroleucine.
Article
Chemistry, Multidisciplinary
Victor Koehler, Maxime Gauthier, Chenhao Yao, Karine Fournel-Marotte, Philip Waeles, Brice Kauffmann, Ivan Huc, Frederic Coutrot, Yann Ferrand
Summary: This study investigates the wrapping of an aromatic oligoamide helix around an active ester-containing [2]rotaxane, which enforces the sliding and sequestration of the surrounding macrocycle around a part of the axle without any formal affinity. The foldamer-mediated compartmentalization of the [2]rotaxane shuttle was subsequently used to prepare an improbable rotaxane.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Chenhao Yao, Brice Kauffmann, Ivan Huc, Yann Ferrand
Summary: Two oligoamide macrocycles composed of eight and twelve monomers were synthesized by utilizing helicity disruptors. The resulting macrocyclic ribbons adopt figure-of-eight and pseudoplectoneme shapes and are capable of self-assembly.
CHEMICAL COMMUNICATIONS
(2022)
