Article
Multidisciplinary Sciences
Richard Mayanja, Adrian Muwonge, Dickson Aruhomukama, Fred Ashaba Katabazi, Mudarshiru Bbuye, Edgar Kigozi, Annettee Nakimuli, Musa Sekikubo, Christine Florence Najjuka, David Patrick Kateete
Summary: This study aimed to investigate the source of EKE organisms for neonates in a maternity ward in Uganda. The results showed evidence of transmission of drug-resistant EKE bacteria in the ward and highlighted the high prevalence of drug resistance genes. This calls for more effective infection prevention/control measures and antimicrobial stewardship programs in hospitals to reduce the spread of drug-resistant bacteria and improve patient outcomes.
Article
Chemistry, Medicinal
Anka Lucic, Philip Hinchliffe, Tika R. Malla, Catherine L. Tooke, Jurgen Brem, Karina Calvopina, Christopher T. Lohans, Patrick Rabe, Michael A. McDonough, Timothy Armistead, Allen M. Orville, James Spencer, Christopher J. Schofield
Summary: Penems have shown potential as antibacterials and beta-lactamase inhibitors, but their clinical use is limited compared to carbapenems. Faropenem, with a C-2 tetrahydrofuran ring, is resistant to some beta-lactamases. Studies on reactions of faropenem with beta-lactamases demonstrate different outcomes, suggesting further research is needed for optimizing the interactions between penems and beta-lactamases.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Infectious Diseases
Neetu Gupta, Kalpana Angadi, Savita Jadhav
Summary: This review aims to understand the mechanisms of carbapenem resistance, categorize and discuss carbapenemases encoding genes and various mobile genetic elements, as well as the prevalence of carbapenemase genes in recent years in A. baumannii from various geographical regions.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Amelia Bhatnagar, Sandra Boyd, Sarah Sabour, Janine Bodnar, Elizabeth Nazarian, Nadine Peinovich, Christine Wagner, Bradley Craft, Paula Snippes Vagnone, Justin Simpson, Victoria N. Stone, Michelle Therrien, Allen Bateman, Danielle Lower, Jennifer Y. Huang, Stephanie Gumbis, David Lonsway, Joseph D. Lutgring, Maria Karlsson, Allison C. Brown
Summary: Aztreonam-avibactam, a drug combination currently in phase 3 clinical trials, has shown potent in vitro activity against metallo-beta-lactamase (MBL)-producing Enterobacterales.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Tomefa E. Asempa, Hannah Bajor, Jessica H. Mullins, Janice Hartnett, David P. Nicolau
Summary: Research on identifying alternative growth media that better mimic host conditions is increasing. This study evaluated meropenem susceptibility against MBL-harboring Enterobacterales strains in urine, finding lower resistance compared to conventional broth. Factors influencing zinc concentrations in urine and their impact on antibiotic resistance warrant further investigation.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Dimitra Petropoulou, Maria Siopi, Sophia Vourli, Spyros Pournaras
Summary: This study evaluated the in vitro activity of the sulbactam-durlobactam (SD) combination against carbapenem-resistant A. baumannii isolates. SD demonstrated the lowest minimum inhibitory concentration (MIC90) and showed potential utility for the treatment of infections caused by A. baumannii. The addition of imipenem further enhanced the activity of SD.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Eduardo Rodriguez-Noriega, Elvira Garza-Gonzalez, Paola Bocanegra-Ibarias, Beatriz Alejandra Paz-Velarde, Sergio Esparza-Ahumada, Esteban Gonzalez-Diaz, Hector R. Perez-Gomez, Rodrigo Escobedo-Sanchez, Gerardo Leon-Garnica, Rayo Morfin-Otero
Summary: This study identified the previous use of antibiotics, the use of a urinary catheter, the use of a central venous catheter, the use of mechanical ventilation, and ICU stay as predictors and independent risk factors for infection with NDM-1 K. pneumoniae.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Xiaopeng Jing, Yanyan Hu, Tingting Wu, Xing Zhang, Shaofeng Luo, Wei Wang, Xiaochun Min, Ruiling Sun, Ji Zeng
Summary: A method using MALDI-TOF MS with EDTA and PB was established to distinguish metallo-beta-lactamase and serine carbapenemases. The method showed high sensitivity and specificity, making it helpful for antibiotic selection in clinical practice.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Dagmara Depka, Agnieszka Mikucka, Tomasz Bogiel, Mateusz Rzepka, Patryk Zawadka, Eugenia Gospodarek-Komkowska
Summary: This study aimed to evaluate different detection methods for carbapenemases and found that both conventional PCR and real-time PCR showed high sensitivity in detecting carbapenem-resistant strains, making them valuable tools for accurate diagnosis.
Article
Microbiology
Jennifer M. Colquhoun, Marjan Farokhyfar, Anna R. Hutcheson, Alexander Anderson, Christopher R. Bethel, Robert A. Bonomo, Anthony J. Clarke, Philip N. Rather
Summary: Beta-lactamase expression is the main resistance mechanism in multidrug-resistant Acinetobacter baumannii, with high-level expression occurring in up to 98.5% of modern isolates. Overexpression of OXA-23 beta-lactamase leads to significant changes in peptidoglycan composition, creating new vulnerabilities and potentially novel antimicrobial targets. This study paves the way for new approaches to treating resistant A. baumannii strains.
Article
Infectious Diseases
Minhas Alam, Muhammad Hidayat Rasool, Iahtasham Khan, Mohsin Khurshid, Bilal Aslam
Summary: This study aimed to investigate the epidemiological characteristics and antimicrobial resistance of Acinetobacter baumannii in cattle in Pakistan. The results showed an overall prevalence of A. baumannii at 3.31%, with carbapenem-resistant isolates identified. This finding highlights the need for an urgent and effective surveillance system to limit the transmission of CRAB in cattle.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Anka Lucic, Tika R. Malla, Karina Calvopina, Catherine L. Tooke, Jurgen Brem, Michael A. McDonough, James Spencer, Christopher J. Schofield
Summary: This study investigated the reaction of the carbapenem biapenem with different subclasses of beta-lactamases. The results showed the formation of enamine and various types of imine products. The findings support the idea that prolonging the lifetime of beta-lactamase carbapenem complexes by optimizing the tautomerization process could lead to improved carbapenems.
Article
Infectious Diseases
Mahnaz Nikibakhsh, Farzaneh Firoozeh, Farzad Badmasti, Kourosh Kabir, Mohammad Zibaei
Summary: The study detected a high prevalence of MBLs genes, especially bla(VIM), in multidrug-resistant A. baumannii isolates from burn patients. Most strains also carried class 1 integrons, and gene cassettes arrays of integrons including cmlA5 and cmlA7 were detected in Iran for the first time in A. baumannii.
BMC INFECTIOUS DISEASES
(2021)
Article
Microbiology
Qian Zhao, Yu Feng, Zhiyong Zong
Summary: Klebsiella pneumoniae strains carrying both carbapenem resistance and hypervirulence genes pose a severe threat to human health. This study reports a novel type of hvCRKP strain, which emerged from the carbapenem-susceptible hypervirulent K. pneumoniae lineage and exhibits unique characteristics compared to other strains. The findings reveal a new mechanism of genetic transfer and emphasize the need for rigorous monitoring and novel countermeasures to curb plasmid-mediated transmission of hvCRKP.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Cheng Chen, Yinsui Xu, Peter Oelschlaeger, Juergen Brem, Lu Liu, Dongmei Wang, Hongzhe Sun, Ke-Wu Yang
Summary: The emergence of multi-drug resistant pathogens co-expressing serine and metallo-carbapenemases significantly reduces the effectiveness of carbapenems. The first SeCN-derived dual inhibitor of serine and metallo-carbapenemases is reported, with IC50 values ranging from 0.0038 to 1.27 & mu;g mL(-1). The inhibitor forms covalent bonds with Cys221 of NDM-1 and Ser70 of KPC-2, achieving selective labelling and cross-class inhibition for carbapenemases. These findings provide a potential strategy to develop clinically useful dual inhibitors targeting serine and metallo-carbapenemases to combat superbugs.
CHEMICAL COMMUNICATIONS
(2023)
Article
Infectious Diseases
Samanta Freire, Teresa Grilo, Patrice Nordmann, Laurent Poirel, Marta Aires-de-Sousa
Summary: A rapid and reliable multiplex PCR was developed to simultaneously detect known plasmid-mediated fos genes conferring acquired resistance to fosfomycin. Our technique was tested on a collection of Escherichia coli isolates previously identified as bearing fosA, fosC, and fosL-like genes, demonstrating a sensitivity and specificity of 100%.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Mustafa Sadek, Remi Le Guern, Eric Kipnis, Philippe Gosset, Laurent Poirel, Rodrigue Dessein, Patrice Nordmann
Summary: This study reports the development of cefiderocol resistance among sequential Pseudomonas aeruginosa clinical isolates, without exposure to the drug. The resistance was driven by multifactorial mechanisms, including mutational changes in iron transporter proteins associated with drug uptake and overexpression of certain genes.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Christophe Le Terrier, Patrice Nordmann, Mustafa Sadek, Laurent Poirel
Summary: This study evaluated the in vitro activity of cefepime/taniborbactam and cefepime/zidebactam against aztreonam/avibactam-resistant E. coli strains. The results showed that cefepime/taniborbactam had a resistance rate of 100% against these strains, while cefepime/zidebactam was effective in all cases. This suggests that cefepime/zidebactam combination could be a promising therapeutic option.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Ahmad Ibrahim, Maxime Bouvier, Mustafa Sadek, Jean-Winoc Decousser, Laurent Poirel, Patrice Nordmann
Summary: Cefiderocol (FDC) is an effective siderophore cephalosporin against multidrug-resistant Gram-negative bacteria. The SuperFDC medium was developed to rapidly and accurately identify FDC-resistant pathogens, which is crucial for controlling their spread. The medium showed high sensitivity and specificity in detecting FDC-resistant isolates with various resistance mechanisms.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Jacqueline Findlay, Laurent Poirel, Patrice Nordmann
Summary: A study was conducted to select MEM-VAB resistant mutants and decipher the resistance mechanisms. The mutants showed reduced susceptibility to various antibiotics and had mutations in the ompK36 gene. Complementation with the wildtype ompK36 gene reduced the minimal inhibitory concentrations. Additionally, the bla KPC gene copy numbers were significantly increased in the resistant mutants.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Letter
Infectious Diseases
Jacqueline Findlay, Juan Bosch Duran, Laurent Poirel, Patrice Nordmann
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Mariana Fernandez-Pittol, Juan Carlos Hurtado, Maryam Ali, Alvar Simarro, Fadiana Proano, Maria Sierra, Jordi Vila
Summary: In various healthcare settings, such as emergency surgery patients, there is a need for rapid detection of SARS-CoV-2. The QuantuMDx Q-POC is an automated fast workflow platform that detects three genes, allowing differentiation between SARS-CoV-2 and other coronaviruses. This study compared QuantuMDx Q-POC with the Cobas 6800 algorithm for SARS-CoV-2 detection, showing high sensitivity and a short detection time of 30 minutes. Overall, the QuantuMDx Q-POC system is a reliable option for rapid SARS-CoV-2 detection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Lucia Boix-Palop, Andrea Vergara, Emma Padilla, Diego Martinez, Ana Blanco, Josefa Perez, Esther Calbo, Jordi Vila, Climent Casals-Pascual
Summary: The aim of this study was to evaluate the diagnostic performance of plasma Lipocalin-2 (LCN2) concentration in adult patients with community-acquired pneumonia (CAP) and its association with etiology, severity, and prognosis. The study found that LCN2 concentration was higher in bacterial CAP compared to non-bacterial CAP and had a limited ability to distinguish between the two. It also found that LCN2 concentration was associated with disease severity in adult patients with CAP. However, its utility as a biomarker to discriminate between viral and bacterial etiology in CAP is limited.
Article
Infectious Diseases
Natalia Roson-Calero, Clara Balleste-Delpierre, Javier Fernandez, Jordi Vila
Summary: In recent decades, there has been an increasing trend in infections caused by multidrug-resistant (MDR) bacteria, which are associated with higher morbidity and mortality. Various interventions should be implemented to reduce the emergence and spread of MDR bacteria. This mini-review aims to compile and discuss different strategies for eradicating MDR bacteria from the gut microbiota.
Article
Infectious Diseases
Teresa Grilo, Samanta Freire, Bruno Miguel, Luis Nogueira Martins, Maria Favila Menezes, Patrice Nordmann, Laurent Poirel, Maria Jose Rego Sousa, Marta Aires-de-Sousa
Summary: This study evaluated the occurrence of plasmid-mediated fos genes among fosfomycin-resistant Escherichia coli isolates collected from patients in Lisbon, Portugal. The results showed a low prevalence of fosfomycin-resistant E. coli isolates, but 15% of these isolates carried transferable fosA genes, and a high rate of extended-spectrum beta-lactamase (ESBL) production was observed.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Eric Fernandez-De La Cruz, Joanna Wessely-Szponder, Miguel Vinas, Teresa Vinuesa, Alexandra Merlos, Marta Jorba, Paula Espinal, Ester Fuste
Summary: This study aimed to explore the antimicrobial activity and mechanisms of action of a porcine neutrophil cathelicidin mixture (MPPN). It was found that MPPN exhibited bactericidal activity against E. coli at high concentrations and bacteriostatic activity at lower concentrations. The interaction of MPPN with lipid bilayers caused alterations in the structure.
Article
Infectious Diseases
Sara Batista, Mariana Fernandez-Pittol, Lorena San Nicolas, Diego Martinez, Marc Rubio, Montserrat Garrigo, Jordi Vila, Griselda Tudo, Julian Gonzalez-Martin
Summary: Patients with chronic pulmonary diseases infected by Mycobacterium avium complex (MAC) often develop complications and suffer from treatment failure due to biofilm formation. There is a lack of correlation between in vitro susceptibility tests and the treatment of clinical isolates producing biofilm. This study found that the use of potential antibiofilm agents (PAAs) in combination with three-drug combinations can effectively reduce the bactericidal concentration in biofilm forms of MAC isolates.
