Article
Infectious Diseases
Sawsan Mohammed Kareem, Israa M. S. Al-kadmy, Saba S. Kazaal, Alaa N. Mohammed Ali, Sarah Naji Aziz, Rabab R. Makharita, Abdelazeem M. Algammal, Salim Al-Rejaie, Tapan Behl, Gaber El-Saber Batiha, Mohamed A. El-Mokhtar, Helal F. Hetta
Summary: The study revealed common quinolone resistance in K. pneumoniae isolates in Baghdad, with frequent mutations in gyrA and parC genes, as well as a high presence of PMQR genes.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Amal Abo M. Kamer, Noha M. Amer, Ahmed A. Abdelmegeed, Gamal M. El Maghraby, Noha M. Gamaleldin
Summary: Researchers used nano-drug delivery systems to enhance the antimicrobial activity of existing antibiotics against Carbapenemase Resistant Enterobacteriaceae (CRE) and extended spectrum beta-lactamases producing bacteria (ESBL). The optimization of surfactant nanovesicles improved the efficacy of meropenem, ertapenem, and tigecycline. The results showed that the surfactant vesicles reduced the MIC values of the drugs and had potential adhesion/fusion with bacterial cells.
Article
Biochemistry & Molecular Biology
Marzieh Hashemian, Hossein Kazemian, Vahab Hassan Kaviar, Somayeh Karamolahi, Ali Nazari, Mohammad Reza Bagheri, Nourkhoda Sadeghifard, Saeed Khoshnood
Summary: The aim of this study was to determine the antibiotic resistance pattern and identification of ESBLs and CRE in bacterial isolates from ICU patients with NIs. Phenotypic tests showed that 76.19% of E. coli and K. pneumoniae isolates produced ESBLs, and 14.28% were identified as CRE producers. PCR analysis revealed a high prevalence of ESBLs, MBLs, and CRE genes. This study identified the presence of bla(OXA-11), bla(OXA-23), and bla(NDM-1) genes in E. coli and K. pneumoniae in Ilam city, Iran for the first time.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Multidisciplinary Sciences
Temenuga Stoeva, Rumiana Markovska, Ivan Mitov
Summary: This study evaluated the epidemiological relationship between carbapenem-resistant (CR) and/or 3rd generation cephalosporin resistant Klebsiella pneumoniae blood isolates collected from patients in the University Hospital in Varna city during the period January 2014 - December 2017. The study also aimed to characterize the ESBLs and carbapenemase production in these isolates. The findings revealed a wide intrahospital dissemination of KPC-2 and/or CTX-M-15 producing Klebsiella pneumoniae, with ST15 persisting throughout the study period, demonstrating high cross transmission, epidemic and invasive potential.
COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES
(2021)
Article
Medical Laboratory Technology
Nabi Jomehzadeh, Khadijeh Ahmadi, Mozhdeh Amiri Bahmanshiri
Summary: This study found significant quinolone resistance in Klebsiella pneumoniae isolates, with most strains showing resistance to nalidixic acid and ofloxacin. MIC analysis revealed a high percentage of isolates resistant to ciprofloxacin, with a majority harboring PMQR genes. The findings suggest a considerable level of resistance against quinolones, potentially due to their extensive and inappropriate use.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Infectious Diseases
Sandip Patil, Hongyu Chen, Chunna Guo, Xiaoli Zhang, Pei-Gen Ren, Ngiambudulu M. Francisco, Feiqiu Wen
Summary: This study investigated the clonal diversity of carbapenemase-producing Klebsiella pneumoniae isolates from the Shenzhen Children's Hospital, China, and found that these isolates were resistant to multiple antibiotics, with bla(NDM) being the most detected gene and ST307 being the predominant sequence type. Plasmid replicon typing revealed that IncFIS, IncHI2, IncFIC, and IncFIA plasmids carried various antibiotic resistance genes.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Morteza Saki, Mansour Amin, Mohammad Savari, Mohammad Hashemzadeh, Seyed Saeid Seyedian
Summary: This study investigated the molecular epidemiology of carbapenem-resistant classic Klebsiella pneumoniae (CR-cKp) and carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) isolates in southwestern Iran. The occurrence of MDR CR-hvKp isolates harboring bla(NDM) and bla(GES) was detected for the first time in this region. Long-term surveillance and more effective treatment strategies are needed to prevent the spread of CR-hvKp.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Sandip Patil, Liu Pai, Xiowen Chen, Ngiambudulu M. Francisco, Hongyu Chen, Yunsheng Chen, Shaowei Dong, Sixi Liu, Feiqiu Wen
Summary: This study reported the co-occurrence of mcr-1 and mcr-3 genes on a single plasmid in pathogenic ESBL/MBL-producing E. coli and K. pneumoniae isolated from young children. These strains were resistant to multiple classes of antibiotics. The results of conjugation experiment and genetic screening confirmed the presence of mcr genes and ESBL/MBL-encoding genes.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Luciana Sampaio Lima, Aldo Aparecido Proietti-Junior, Yan Correa Rodrigues, Marcelo Cleyton da Silva Vieira, Luana Nepomuceno Gondim Costa Lima, Cintya de Oliveira Souza, Veronica Dias Goncalves, Marcelo de Oliveira Lima, Dalia dos Prazeres Rodrigues, Karla Valeria Batista Lima
Summary: This study investigated the prevalence of quinolone-resistant Escherichia coli in fish species in the Brazilian Amazon. The isolates exhibited multidrug resistance, virulence, and genetic diversity. The results suggest that fish species in the Brazilian Amazon may serve as a reservoir for antimicrobial-resistant strains.
Article
Immunology
Hamid Talebzadeh, Hamid Mellali, Hamid Solgi
Summary: This study investigated the prevalence of drug resistance and virulence determinants in drug-resistant K. pneumoniae strains. The results showed that the strains were generally multidrug-resistant and carried at least one ESBL resistance determinant. Additionally, multiple mutations and virulence genes were found, and some strains exhibited a hypervirulent phenotype. Overall, these drug-resistant K. pneumoniae strains with multiple virulence factors pose a threat to the treatment of hospitalized patients.
ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA
(2022)
Article
Obstetrics & Gynecology
Xiao-Mei Zhang, He Wang, Ming-Cheng Li, Xue-Ying Bao, Jia-Yu Chen
Summary: This study investigated the susceptibility patterns and genotype of extended-spectrum-beta-lactamase (ESBL)-producing Klebsiella pneumoniae isolates in obstetrics & gynaecology settings in three associated hospitals of a university in China. The high prevalence of ESBLs with diverse enzyme gene types indicates the necessity of screening changes in ESBL-producing isolates in the region.
CLINICAL AND EXPERIMENTAL OBSTETRICS & GYNECOLOGY
(2021)
Article
Microbiology
Qiwei Chen, Lizhang Liu, Xiaofang Hu, Xu Jia, Xiaowei Gong, Youjun Feng, Man Huang
Summary: The study focuses on the diversity of KPC-2-producing plasmids in highly transmissible ST11 clones of carbapenem-resistant K. pneumoniae (CRKP). The researchers report a clinical case of a rare ST437 CRKP isolate, K186, which produces KPC-2. Whole-genome sequencing revealed three distinct resistance plasmids, one of which, pK186_KPC, is a small IncN-type plasmid of 26,012bp. The study further suggests that the carbapenem-resistant pK186_KPC might have arisen from the integration of ancestral IncN and IncFII plasmids.
MICROBIOLOGY SPECTRUM
(2022)
Article
Pharmacology & Pharmacy
Mustafa M. Abdulkareem, Masood A. Abdulrahman, Najim A. Yassin
Summary: This study aimed to detect five plasmid-mediated quinolone resistance (PMQR) genes in fluoroquinolone (FQs)-resistant Klebsiella pneumoniae isolated from clinical specimens. Among 120 K. pneumoniae isolates, 68 FQs-resistant isolates were included in the molecular study. The results showed a high prevalence of PMQR genes, with the Aac (6')-Ib-cr gene being the most predominant.
Article
Immunology
Yi Zhou, Yue Cheng, Tianyou Ma, Jun Wang, Shaoru Li, Jingdan Wang, Lei Han, Xinyao Hou, Xinxin Ma, Sijin Jiang, Pu Li, Jia Lv, Bei Han, Rong Da
Summary: In this study, a novel ST3355 ESBL-HvKP strain HKE9 was identified with high virulence and drug resistance. The global regulatory factor RpoS was found to affect the strain's resistance to antimicrobials and environmental adaptability, and regulated by T4SS. Additionally, rpoS also influenced the strain's competitiveness, cell adhesion ability, and sensitivity to disinfectants.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Jose Joao Sumbana, Antonella Santona, Nader Abdelmalek, Maura Fiamma, Massimo Deligios, Alice Manjate, Jahit Sacarlal, Salvatore Rubino, Bianca Paglietti
Summary: Globally, antibiotic-resistant Klebsiella spp. cause healthcare-associated infections with high mortality rates. The study investigated the phenotypic and genetic features of Klebsiella isolates from invasive infections in Mozambique. The majority of isolates displayed multidrug resistance, with ESBL-producing strains carrying various beta-lactamase genes. The study also identified susceptible hypervirulent Klebsiella pneumoniae isolates associated with severe infections. The findings highlight the importance of surveillance strategies to prevent the emergence of multidrug-resistant hypervirulent Klebsiella pneumoniae.
Editorial Material
Immunology
Jesus Rodriguez-Bano, Belen Gutierrez-Gutierrez
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Francesc Escrihuela-Vidal, Achim J. Kaasch, Maja Von Cube, Siegbert Rieg, Winfried V. Kern, Harald Seifert, Kyoung-Ho Song, Chun-Hsing Liao, Robert Tilley, Hannah Gott, Matt Scarborough, Claire Gordon, Martin J. Llewelyn, Richard Kuehl, Laura Morata, Alex Soriano, Jonathan Edgeworth, Enrique Ruiz De Gopegui, Emmanuel Nsutebu, Jose Miguel Cisneros, Vance G. Fowler, Guy Thwaites, Joaquin Lopez-Contreras, Gavin Barlow, Hugo Guillermo Ternavasio-De La Vega, Jesus Rodriguez-Bano, Luis Eduardo Lopez-Cortes
Summary: This study analysed the adherence and impact of quality-of-care indicators (QCIs) in the management of Staphylococcus aureus bloodstream infection. The results showed that adherence to QCIs was associated with reduced 90-day mortality.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Review
Oncology
Elena Carrara, Elisa Razzaboni, Anna Maria Azzini, Maria Elena De Rui, Mariana Nunes Pinho Guedes, Anna Gorska, Maddalena Giannella, Linda Bussini, Michele Bartoletti, Federica Arbizzani, Zaira R. Palacios-Baena, Giulia Caponcello, Natalia Maldonado, Jesus Rodriguez-Bano, Carlo Visco, Mauro Krampera, Evelina Tacconelli
Summary: The main aim of this systematic review is to quantify the risk and identify predictors of clinical progression of SARS-CoV-2 in hematological patients compared to different control populations. The study found that hematological patients had a higher risk of COVID-19-related mortality, hospitalization, intensive-care admission, and mechanical ventilation compared to controls. Older age, male sex, and hypertension were significantly related to worse clinical outcomes in hematological patients with COVID-19.
HEMATOLOGICAL ONCOLOGY
(2023)
Article
Infectious Diseases
S. Diaz-Diaz, Pilar Yerbes, E. Recacha, B. de Gregorio-Iaria, Marina R. Pulido, Maria Romero-Munoz, F. Docobo-Perez, A. Pascual, J. M. Rodriguez-Martinez
Summary: RecA inhibition is a potential strategy to combat antimicrobial resistance. This study investigates the impact of RecA inactivation on heteroresistance in Escherichia coli clinical isolates. The results show that deletion of the recA gene significantly reduces heteroresistant subpopulations, especially against quinolones or beta-lactams. The molecular basis of heteroresistance is associated with an increase in copy number of resistance genes, which is suppressed in the absence of the recA gene.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Letter
Infectious Diseases
Sonsoles Salto-Alejandre, Marta Carretero-Ledesma, Pedro Camacho-Martinez, Judith Berastegui-Cabrera, Carmen Infante, Regino Rodriguez-Alvarez, Jorge Alba, Patricia Perez-Palacios, Emilio Garcia-Diaz, Cristina Roca, Julia Praena, Maria Jose Blanco-Vidal, Sonia Santibanez, Rocio Valverde-Ortiz, Javier Nieto-Arana, Concepcion Garcia-Garcia, David Gutierrez-Campos, Natalia Maldonado, Gabriel Bernal, Miguel Angel Gomez-Bravo, Jose Manuel Sobrino, Manuela Aguilar-Guisado, Rocio Alvarez-Marin, Josune Goikoetxea-Aguirre, Jose Antonio Oteo, Zaira R. Palacios-Baena, Alvaro Pascual, Jose Antonio Lepe, Jesus Rodriguez-Bano, Jose Miguel Cisneros, Jeronimo Pachon, Javier Sanchez-Cespedes, Elisa Cordero
JOURNAL OF INFECTION
(2023)
Article
Infectious Diseases
Nerea Castillo-Fernandez, Pedro Maria Martinez Perez-Crespo, Elena Salamanca-Rivera, Laura Herrera-Hidalgo, Aristides de Alarcon, Maria Dolores Navarro-Amuedo, Teresa Marrodan Ciordia, Maria Teresa Perez-Rodriguez, Juan Sevilla-Blanco, Alfredo Jover-Saenz, Jonathan Fernandez-Suarez, Carlos Arminanzas-Castillo, Jose Maria Reguera-Iglesias, Clara Natera Kindelan, Lucia Boix-Palop, Eva Leon Jimenez, Fatima Galan-Sanchez, Alfonso del Arco Jimenez, Alberto Bahamonde-Carrasco, David Vinuesa Garcia, Alejandro Smithson Amat, Jordi Cuquet Pedragosa, Isabel Maria Reche Molina, Ines Perez Camacho, Esperanza Merino de Lucas, Belen Gutierrez-Gutierrez, Jesus Rodriguez Bano, Luis Eduardo Lopez Cortes
Summary: A post-hoc analysis of two Spanish cohorts showed that sequential OPAT is as safe and effective as conventional hospitalization for treating patients with S. aureus bacteremia.
Article
Medicine, General & Internal
Adi Turjeman, Elodie von Dach, Jose Molina, Erica Franceschini, Fidi Koppel, Dana Yelin, Yael Dishon-Benattar, Cristina Mussini, Jesus Rodriguez-Bano, Jose Miguel Cisneros, Angela Huttner, Mical Paul, Leonard Leibovici, Dafna Yahav
Summary: This study aims to compare the effect of short and long-term treatment in Gram-negative bacteremia on all-cause mortality in pre-specified subgroups. The results show that in patients with stable hemodynamics and normal temperature for 48 hours prior to discontinuation, seven days of antibiotic therapy for enterobacterales bacteremia result in similar outcomes as 14 days.
Article
Surgery
Elena Perez-Nadales, Mario Fernandez-Ruiz, Alejandra M. Natera, Belen Gutierrez-Gutierrez, Alessandra Mularoni, Giovanna Russelli, Ligia Camera Pierrotti, Maristela Pinheiro Freire, Marco Falcone, Giusy Tiseo, Mario Tumbarello, Francesca Raffaelli, Edson Abdala, Marta Bodro, Elena Gervasi, Maria Carmen Farinas, Elena M. Seminari, Juan Jose Caston, Juan Antonio Marin-Sanz, Victor Galvez-Soto, Meenakshi M. Rana, Belen Loeches, Pilar Martin-Davila, Alvaro Pascual, Jesus Rodriguez-Bano, Jose Maria Aguado, Luis Martinez-Martinez, Julian Torre-Cisneros, REIPI INCREMENT-SOT Study Grp
Summary: This study compared the efficacy of ceftazidime-avibactam (CAZ-AVI) and the best available therapy (BAT) in treating solid organ transplant recipients with bloodstream infection caused by carbapenemase-producing Klebsiella pneumoniae. The results showed that patients treated with CAZ-AVI had higher clinical success rates at 14-day and 30-day and lower 30-day mortality compared to those receiving BAT.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Microbiology
S. Diaz-Diaz, E. Recacha, Marina R. Pulido, Maria Romero-Munoz, B. de Gregorio-Iaria, F. Docobo-Perez, A. Pascual, J. M. Rodriguez-Martinez
Summary: The suppression of the SOS response and Dam methylation system enhances the in vitro activity of quinolones. Dual targeting of the recA (SOS response) and Dam methylation system genes increases the sensitivity of Escherichia coli to quinolones, even in a resistant strain model. This genetic and microbiological approach demonstrates the potential for preventing the evolution of resistance by suppressing both systems in quinolone-resistant strains.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Maddalena Giannella, Manuel Huth, Elda Righi, Jan Hasenauer, Lorenzo Marconi, Angelina Konnova, Akshita Gupta, An Hotterbeekx, Matilda Berkell, Zaira R. Palacios-Baena, Maria Cristina Morelli, Mariarosa Tame, Marco Busutti, Luciano Potena, Elena Salvaterra, Giuseppe Feltrin, Gino Gerosa, Lucrezia Furian, Patrizia Burra, Salvatore Piano, Umberto Cillo, Mara Cananzi, Monica Loy, Gianluigi Zaza, Francesco Onorati, Amedeo Carraro, Fiorella Gastaldon, Maurizio Nordio, Samir Kumar-Singh, Jesus Rodriguez Bano, Tiziana Lazzarotto, Pierluigi Viale, Evelina Tacconelli
Summary: This study aimed to assess the predictors of negative antibody response in solid organ transplant recipients after the first booster of SARS-CoV-2 vaccination. Machine learning models and clinical data were used to identify age, type of transplant, time between transplant and vaccination, immunosuppressive drugs, type of vaccine, and graft function as significant predictors of negative antibody response. This study is of significant importance for improving vaccination strategies in solid organ transplant recipients.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Microbiology
T. Blanco-Martin, J. Guzman-Puche, C. Riazzo, M. Gasca-Santiyan, M. Hernandez-Garcia, R. Canton, J. Torre-Cisneros, C. Herrera, L. Martinez-Martinez
Summary: IMI carbapenemases confer an unusual resistance pattern, making them difficult to detect. Commercial molecular methods usually do not include bla(IMI) genes, contributing to the hidden dissemination of bacteria producing these enzymes. We report a plasmid-encoded IMI-6 carbapenemase in an Enterobacter ludwigii isolate, providing insights into its genetic characteristics.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
M. Delgado-Valverde, I. Portillo-Calderon, E. Recacha, P. Perez-Palacios, A. Pascual
Summary: This study compared the in vitro activity of cefiderocol with other antimicrobials against MBL-GNB isolates from hospitals in Andalusia, Spain. Cefiderocol showed potent activity against all isolates tested, regardless of breakpoint values.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Beatriz Fernandez-Rubio, Laura Herrera-Hidalgo, Luis E. Lopez-Cortes, Rafael Luque-Marquez, Aristides De Alarcon, Sonia Luque-Pardos, Aurora Fernandez-Polo, Jose Maria Gutierrez-Urbon, Jesus Rodriguez-Bano, Maria Victoria Gil-Navarro, Alicia Gutierrez-Valencia
Summary: This study evaluated the physical and chemical stability of temocillin at different temperatures and stored in different containers at the recommended dose for OPAT. It was found that temocillin is stable under OPAT conditions and can be an appropriate treatment option for patients. A novel LC-MS/MS method was also developed for the quantification of temocillin.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Irene Sanchez-Leon, Teresa Garcia-Martinez, Seydina M. Diene, Elena Perez-Nadales, Luis Martinez-Martinez, Jean-Marc Rolain
Summary: This study aimed to investigate heteroresistance in nine clinical isolates of Klebsiella pneumoniae producing OXA-48 and describe genomic changes in vitro. It was found that the heteroresistance in K. pneumoniae isolates was mainly attributed to insertions in the mgrB gene and point mutations in colistin resistance proteins. These findings contribute to the understanding of colistin resistance mechanisms in OXA-48-producing K. pneumoniae mutants.
Article
Microbiology
Irene Sanchez-Leon, Elena Perez-Nadales, Juan Antonio Marin-Sanz, Teresa Garcia-Martinez, Luis Martinez-Martinez
Summary: This study investigated heteroresistance in 10 wild-type Klebsiella pneumoniae isolates of clinical origin. The results showed the importance of persistent variants and stable mutants in heteroresistance, and described the genomic changes observed in mutants obtained in population analysis profiling (PAP) assays.
MICROBIOLOGY SPECTRUM
(2023)