Article
Microbiology
Kyle A. Hess, Kirstin Kooda, Joshua D. Shirley, Audrey N. Schuetz, Omar Abu Saleh, Ryan W. Stevens
Summary: Genotypic testing for mecA/mecC is crucial for optimizing antimicrobial therapy in Staphylococcus aureus infections. However, little is known about the optimal reporting and therapy for patients with phenotypic oxacillin resistance but lacking genotypic evidence of mecA or mecC.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Veterinary Sciences
Isabela Carvalho dos Santos, Lidiane Nunes Barbosa, Gustavo Ratti da Silva, Luciana Kazue Otutumi, Melissa Marchi Zaniolo, Mariana Carvalho dos Santos, Larissa Rafaela de Paula Ferreira, Daniela Dib Goncalves, Lisiane de Almeida Martins
Summary: The aim of this study was to verify the bacterial resistance profile and detect the presence of mecA gene in Staphylococcus spp. isolated from the nasal microbiota of domiciled dogs. The results showed that domiciled dogs carry multidrug-resistant Staphylococcus spp., which may be a potential source of human contamination.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Bingshao Liang, Zhile Xiong, Zhuwei Liang, Chao Zhang, Hao Cai, Yan Long, Fei Gao, Jielin Wang, Qiulian Deng, Huamin Zhong, Yongqiang Xie, Lianfen Huang, Sitang Gong, Zhenwen Zhou
Summary: The oxacillin- and cefoxitin-susceptible mecA-positive Staphylococcus aureus is a novel type of stealth MRSA that is difficult to detect using conventional methods. Whole-genome sequencing of these strains revealed mutations in the mecA gene that resulted in premature stop codon formation and a base substitution in the second promoter region. These mutations make MRSA strains difficult to detect, emphasizing the importance of combining phenotype tests with mecA or penicillin-binding protein 2a detection for MRSA identification.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Pak-Leung Ho, Ying-Hang Law, Melissa Chun-Jiao Liu, Andes Lau, Man-Ki Tong, Kin-Hung Chow, Alan Ka-Lun Wu, Cindy Wing-Sze Tse, Vincent Chi-Chung Cheng, Tak-Lun Que
Summary: The new oxacillin agar reliably differentiated mecA-positive and mecA-negative isolates in S. lugdunensis, while cefoxitin DD and MIC tests showed limitations in reliable detection of some mecA-positive S. lugdunensis isolates.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Erzsebet Takacs, Jianlong Wang, Libing Chu, Tunde Toth, Krisztina Kovacs, Aniko Bezsenyi, Laszlo Szabo, Renata Homlok, Laszlo Wojnarovits
Summary: The study found that oxacillin is poorly biodegradable and non-toxic, potentially contributing to the spread of beta-lactam antibiotic resistant bacteria. In contrast, the products formed by gamma-irradiation were easily biodegradable and did not show antibacterial activity. The attack of hydrated electron takes place on the carbonyl groups in both antibiotics.
Article
Chemistry, Medicinal
Tanveer Ali, Abdul Basit, Asad Mustafa Karim, Jung-Hun Lee, Jeong-Ho Jeon, Shafiq ur Rehman, Sang-Hee Lee
Summary: This study investigates the diversity and role of missense mutations of PBP2a in the antibiotic resistance mechanism in Staphylococcus aureus. The results show that the E239R mutation in the allosteric site of PBP2a induces conformational changes in the active site, hindering its interaction with cefoxitin and leading to high-level resistance.
Article
Infectious Diseases
Roushan Liu, Jian Zhang, Xiaoli Du, Yingying Lv, Xiangyu Gao, Yanyan Wang, Junrui Wang
Summary: This study found a relatively low prevalence of OS-MRSA, but with universal heterogeneous resistance and weak PBP2a protein expression. The frequency of resistant subpopulations varied among different isolates, and no dominant clones were identified.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Genetics & Heredity
Michael H. Guo, Laurent C. Francioli, Sarah L. Stenton, Julia K. Goodrich, Nicholas A. Watts, Moriel Singer-Berk, Emily Groopman, Philip W. Darnowsky, Matthew Solomonson, Samantha Baxter, Grace Tiao, Benjamin M. Neale, Joel N. Hirschhorn, Heidi L. Rehm, Mark J. Daly, Anne O'Donnell-Luria, Konrad J. Karczewski, Daniel G. MacArthur, Kaitlin E. Samocha
Summary: This study developed a strategy to infer the phase for rare variant pairs within genes using genotypes observed in the Genome Aggregation Database. The approach showed high accuracy in determining phase in both trio data and patients with Mendelian conditions, providing a valuable resource for interpreting rare co-occurring variants in the context of recessive diseases.
Article
Biochemistry & Molecular Biology
Chaowei Zhang, Kerry Reid, Arthur F. Sands, Antoine Fraimout, Mikkel Heide Schierup, Juha Merila
Summary: Mutation rate is a critical parameter in population genetics, but accurate estimates for wild organisms are limited. This study estimated mutation rates in two marine populations of nine-spined stickleback using pedigrees, whole-genome resequencing, and a high-quality reference genome. The results showed high levels of parental mosaicism and increased estimated divergence times after recalibration with the mutation rate.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Virology
Ivailo Alexiev, Marta Giovanetti, Eleonora Cella, Ivan Ivanov, Ivan Stoikov, Deyan Donchev, Lubomira Grigorova, Anna Gancheva, Reneta Dimitrova, Nelly Korsun, Ivelina Trifonova, Ivva Philipova, Veselin Dobrinov, Iliana Grigorova, Todor Kantardjiev, Iva Christova, Massimo Ciccozzi
Summary: The emergence of the SARS-CoV-2 variant AY.4.2, known as Delta Plus, with additional mutations in the spike protein, has raised concerns about accelerated transmission during the evolving COVID-19 pandemic. This study utilizes genomic epidemiology to investigate the introduction of AY.4.2 in Bulgaria and the wider dissemination of the AY.4.2.1 sublineage in both Bulgaria and the United Kingdom.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Infectious Diseases
Deepa Nair, Nandita Shashindran, Anil Kumar, Vivek Vinodh, Lalitha Biswas, Raja Biswas
Summary: This study aimed to compare various phenotypic methods of detecting methicillin resistance in Staphylococcus aureus with PCR, and to detect oxacillin-susceptible MRSA. Among the different methods studied, latex agglutination showed the highest sensitivity and specificity. It is recommended to combine cefoxitin disk diffusion test with MRSA ID agar or latex agglutination in areas with a high prevalence of OS-MRSA to improve sensitivity.
MICROBIAL DRUG RESISTANCE
(2021)
Article
Biochemistry & Molecular Biology
Richard J. Wang, Muthuswamy Raveendran, R. Alan Harris, William J. Murphy, Leslie A. Lyons, Jeffrey Rogers, Matthew W. Hahn
Summary: The mutation rate is a fundamental evolutionary parameter that affects the health and function of individuals. A study on domestic cats found that their mutation rate is 28% lower than that of humans, but consistent with their shorter generation time. Older sires were found to transmit more mutations.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Editorial Material
Biochemistry & Molecular Biology
Christina M. J. E. Vandenbroucke-Grauls, Jan A. J. W. Kluytmans
Summary: Antibiotic resistance genes evolve in the environment, animals, and humans, and it is crucial to take strategic action in all three areas to understand and limit resistance.
Article
Biochemistry & Molecular Biology
Daniel E. Platt, Hovig Artinian, Francis Mouzaya, Wissam Khalil, Francois G. Kamar, Elizabeth Matisoo-Smith, Francesc Calafell, Nassim Nicolas Taleb, Pierre Zalloua
Summary: There are 18 different religious communities living in Lebanon, showing genetic isolation and autosomal clustering. Analysis of Y-chromosome STR and SNP data reveals a Levantine branching around 5000 years ago and distinct genetic components in the North Lebanese Mountain Maronite community. The Levantine L1b group split from the Caucasus ancestral group around 7300 years ago, with cultural barriers marked by the transmission of L1b lineages.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Infectious Diseases
Irena Murinova, Martin Svidrnoch, Tomas Gucky, Jan Hlavac, Pavel Michalek, Ondrej Slanar, Martin Sima
Summary: This study describes the population pharmacokinetics of oxacillin and proposes continuous infusion and dosing based on renal function for optimal achievement of target PK/PD values.
Article
Microbiology
Ambre Jousselin, Caroline Manzano, Alexandra Biette, Patricia Reed, Mariana G. Pinho, Adriana E. Rosato, William L. Kelley, Adriana Renzoni
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2016)
Article
Microbiology
Sofia Santos Costa, Miguel Viveiros, Adriana E. Rosato, Jose Melo-Cristino, Isabel Couto
Article
Microbiology
P. Gagetti, F. Pasteran, M. P. Martinez, M. Fatouraei, J. Gu, R. Fernandez, L. Paz, W. E. Rose, A. Corso, A. E. Rosato
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2016)
Article
Microbiology
Adriana Renzoni, William L. Kelley, Roberto R. Rosato, Maria P. Martinez, Melanie Roch, Maryam Fatouraei, Daniel P. Haeusser, William Margolin, Samuel Fenn, Robert D. Turner, Simon J. Foster, Adriana E. Rosato
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2017)
Article
Microbiology
S. Wesley Long, Randall J. Olsen, Shrenik C. Mehta, Timothy Palzkill, Patricia L. Cernoch, Katherine K. Perez, William L. Musick, Adriana E. Rosato, James M. Musser
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2014)
Article
Microbiology
Regina Fernandez, Liliana I. Paz, Roberto R. Rosato, Adriana E. Rosato
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2014)
Article
Immunology
Melissa A. Dean, Randall J. Olsen, S. Wesley Long, Adriana E. Rosato, James M. Musser
INFECTION AND IMMUNITY
(2014)
Article
Dentistry, Oral Surgery & Medicine
C. Anaya-Bergman, A. Rosato, J. P. Lewis
MOLECULAR ORAL MICROBIOLOGY
(2015)
Article
Multidisciplinary Sciences
Lucia Palacios, Helena Rosado, Vicente Micol, Adriana E. Rosato, Patricia Bernal, Raquel Arroyo, Helen Grounds, James C. Anderson, Richard A. Stabler, Peter W. Taylor
Article
Multidisciplinary Sciences
Roberto R. Rosato, Regina Fernandez, Liliana I. Paz, Christopher R. Singh, Adriana E. Rosato
Article
Veterinary Sciences
Paula Gagetti, Alice R. Wattam, Gabriela Giacoboni, Adriana De Paulis, Eugenia Bertona, Alejandra Corso, Adriana E. Rosato
BMC VETERINARY RESEARCH
(2019)
Article
Infectious Diseases
Katarzyna E. Greber, Melanie Roch, Mauro A. Rosato, Maria P. Martinez, Adriana E. Rosato
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2020)
Article
Pathology
Adriana E. Rosato, Engy Msiha, Bruce Weng, Michael Mesisca, Ronaldo Gnass, Silvia Gnass, Cedric Bol, Arnold Tabuenca, Roberto R. Rosato
Summary: The emergence of the B.1.617.2 (Delta) variant of the SARS-CoV-2 virus has caused a surge in cases, especially in India. This variant is more transmissible and potentially more severe, leading to an increase in hospitalizations. The study focuses on validating and implementing a rapid detection method for the B.1.617.2 (Delta) variant, which can be easily implemented in clinical laboratories with limited resources.
Article
Infectious Diseases
Paula Gagetti, Roberto R. Rosato, Adriana E. Rosato
Summary: This study elucidates the molecular basis of the dissociated phenotype between oxacillin and cefoxitin antibiotics in Staphylococcus pseudintermedius, showing that PBP4 is directly associated with this phenomenon.
Article
Infectious Diseases
Mariana Andrade, Ketlyn Oliveira, Catarina Morais, Patricia Abrantes, Constanca Pomba, Adriana E. Rosato, Isabel Couto, Sofia Santos Costa
Summary: This study reveals the frequent biofilm production by antimicrobial-resistant coagulase-positive staphylococci in companion animals, which is associated with a higher virulence potential and persistent or recurrent infections.