Article
Multidisciplinary Sciences
Natkamon Saenhom, Rada Kansan, Peechanika Chopjitt, Parichart Boueroy, Rujirat Hatrongjit, Anusak Kerdsin
Summary: Methicillin-resistant staphylococci (MRS), including MRSA and MRCoNS, have a significant impact on public health, causing morbidity, mortality, and economic costs. Accurate detection of MRS is crucial for effective treatment and control measures. A newly developed screening broth showed good validity for MRSA/MSSA detection, but poor validity for MRCoNS/MSCoNS.
Article
Microbiology
Pak-Leung Ho, Ying-Hang Law, Melissa Chun-Jiao Liu, Andes Lau, Man-Ki Tong, Kin-Hung Chow, Alan Ka-Lun Wu, Cindy Wing-Sze Tse, Vincent Chi-Chung Cheng, Tak-Lun Que
Summary: The new oxacillin agar reliably differentiated mecA-positive and mecA-negative isolates in S. lugdunensis, while cefoxitin DD and MIC tests showed limitations in reliable detection of some mecA-positive S. lugdunensis isolates.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
A. Schnitt, T. Lienen, H. Wichmann-Schauer, B-A Tenhagen
Summary: This study found the occurrence of MR-NAS on dairy farms in Germany, with varying positive rates among different types of samples and in the environment. Nine species of MR-NAS were identified. Similar mobile genetic elements were detected in MR-NAS and MRSA on some farms, suggesting potential transfer of resistance genes between NAS and Staph. aureus on these farms.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Nahal Hadi, Sedigheh Nakhaeitazreji, Farshad Kakian, Zahra Hashemizadeh, Alireza Ebrahiminezhad, Jun Wei Roy Chong, Aydin Berenjian, Pau Loke Show
Summary: The synergistic effects of antimicrobial nanostructures with antibiotics provide a promising solution to overcome MRSA resistance. The study found that iron-coated silver nanoparticles (Fe@AgNPs) synergistically enhanced the efficacy of cefoxitin against MRSA, reversing resistance in 90% of infections. These findings are of significant importance for future treatment strategies against MRSA infections.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
W. G. Duan, X. Liu, Z. Na, L. Zhou, F. M. Huang, Q. S. Song, Q. F. Fan
Summary: Oroxylum indicum is a valuable Dai pharmaceutical used for treating acute cough and sore throat. Compounds extracted from O. indicum showed antibacterial activities, especially baicalein exhibited strong activity against MRSA strains. This study provides new evidence for the traditional use of O. indicum in Dai peoples' life.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Biology
Usasom Khongsri, Peerawit Chongrattanameteekul, Sineenart Chantarachart, Kornravee Photichai, Nittaya Chanayat, Thanya Varinrak, Raktham Mektrirat, Nuttawan Srifawattana
Summary: The prevalence of MRSP causing pyoderma is changing gradually, with annual variations. Research on cotrimazole susceptibility to MRSP is limited, but the empirical use of cotrimazole remains interesting. This study aimed to evaluate the susceptibility of cotrimazole to canine pyoderma MRSP isolates.
Article
Agriculture, Dairy & Animal Science
Nadia Safia Chenouf, Olouwafemi Mistourah Mama, Chafik Redha Messai, Laura Ruiz-Ripa, Rosa Fernandez-Fernandez, Isabel Carvalho, Abdelghani Zitouni, Ahcene Hakem, Carmen Torres
Summary: This study investigated antimicrobial resistance and virulence factors in Staphylococcus aureus and coagulase-negative staphylococci in unpasteurized milk in Djelfa, Algeria. The results showed contamination of milk samples with these bacteria, with some strains exhibiting resistance to multiple drugs and carrying various resistance genes.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Infectious Diseases
Duangdaow Khunbutsri, Nattakarn Naimon, Khomson Satchasataporn, Natnaree Inthong, Sarawan Kaewmongkol, Samak Sutjarit, Chanokchon Setthawongsin, Nattakan Meekhanon
Summary: This study aimed to investigate the potential antibacterial activity of Solanum torvum leaf extract against multidrug-resistant Staphylococcus isolated from clinically healthy dogs. The results showed that the leaf extract displayed inhibitory effects, especially when used in combination with oxacillin.
Review
Veterinary Sciences
Chahrazed Belhout, Rachid Elgroud, Patrick Butaye
Summary: Staphylococci, including methicillin-resistant strains, are recognized as important opportunistic pathogens that pose a significant risk to human and animal health. This study aims to provide an overview of the prevalence and distribution of methicillin-resistant staphylococci (MRS) in Arab countries, where there is currently a lack of information on this topic. The increasing observation of MRS in different animal species raises concerns about their impact on animal health and the potential for zoonotic transmission. The availability of comprehensive data on MRS in animals and food products worldwide is crucial for understanding their relevance in the One Health domain.
VETERINARY SCIENCES
(2022)
Article
Multidisciplinary Sciences
Rasoul Mirzaei, Rasoul Yousefimashouf, Mohammad Reza Arabestani, Iraj Sedighi, Mohammad Yousef Alikhani
Summary: The study found that subinhibitory concentrations of cloxacillin, cefazolin, and clindamycin can induce biofilm production in methicillin-resistant Staphylococcus epidermidis (MRSE) strains, while subinhibitory concentrations of vancomycin do not increase biofilm formation. Sub-MIC of antibiotics also up-regulated the expression of biofilm-associated genes.
Article
Biochemistry & Molecular Biology
Fahad Alshabrmi, Eid A. Alatawi
Summary: This study explores the resistance mechanism of MRSA against Cefoxitin using molecular docking and MD simulations. The findings suggest that mutant forms of PBP2a affect the activity of Cefoxitin, and different mutations have different effects on the stability of Cefoxitin binding.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Microbiology
Aline Cristine Magalhaes Costa Messias, Aline Rodrigues Gama, Leticia Suriano de Almeida Prado, Paulo Alex Neves da Silva, Arlindo Rodrigues Galvao Filho, Clarimar Jose Coelho, Melissa Ameloti Gomes Avelino, Jose Daniel Goncalves Vieira, Lilian Carla Carneiro
Summary: This study aimed to explore an alternative method for identifying resistance to oxacillin/cefoxitin in S. aureus from hospitalized children with recurrent tonsillitis. Through 16S rRNA sequencing and antibiogram testing, a feasible separation of resistance based on HSI methodology was obtained, and the MRSA S. aureus characteristics were found. The samples that showed phenotypic resistance to oxacillin/cefoxitin were clearly separated from those that did not, and the PLS-DA model successfully predicted the presence of resistance.
Article
Veterinary Sciences
Dharitree Sonowal, Sandeep Ghatak, Acheenta Gohain Barua, Srinivas Kandhan, Razibuddin Ahmed Hazarika, Arnab Sen, Samir Das, Sarat Sonowal, Rajeev Kumar Sharma, Shantanu Tamuly, Chimanjita Phukan, Ajanta Sharma, Poznur Hussain
Summary: Staphylococcus aureus is a Gram positive bacteria that rapidly acquires antibiotic resistance and poses a major challenge to clinicians worldwide. Methicillin resistant Staphylococcus aureus (MRSA) infections are associated with increased mortality and treatment prolongation. Samples from diverse sources were collected and screened for MRSA, with a higher incidence observed in goats (42.00%), followed by cattle (13.50%), humans (12.90%), and dogs (12.90%). Dogs had a significantly higher incidence of MRSA compared to other hosts. All S. aureus isolates were further screened for the nuc gene, with varying incidence rates observed among different hosts. The study indicates a possible risk of zoonotic transmission of MRSA from canines.
VETERINARY RESEARCH FORUM
(2023)
Article
Microbiology
Rosa Fernandez-Fernandez, Idris Nasir Abdullahi, Carmen Gonzalez-Azcona, Adriana Ulloa, Agusti Martinez, Sara Garcia-Vela, Ursula Hofle, Myriam Zarazaga, Carmen Lozano, Carmen Torres
Summary: A total of 259 staphylococci strains were isolated from nasotracheal samples of 87 healthy nestling white storks, and their antimicrobial activity was tested using the spot-on-lawn method. Extracts from these strains were also tested against indicator bacteria. Results showed that 9 strains exhibited antimicrobial activity, primarily against Gram-positive bacteria. PCR and sequencing analysis revealed the presence of lantibiotic-like encoding genes in these strains.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Abdul Rauf Tareen, Rabaab Zahra
Summary: This study investigated the carriage of methicillin-resistant Staphylococci in wild birds from Islamabad region and found that these birds may disseminate resistant strains into the environment. The findings strongly recommend monitoring of resistant bacteria in wild birds and wildlife.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)