Article
Immunology
Anthony Sophonsri, Corey Kelsom, Mimi Lou, Paul Nieberg, Annie Wong-Beringer
Summary: The study found that patients coinfected with CRKP and another carbapenem-resistant pathogen have more significant morbidity and require longer hospital stays compared to those with CRKP monoinfection. Empiric therapy targeting both CRKP and carbapenem-resistant Pseudomonas aeruginosa may be beneficial for at-risk patients with pneumonia.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Dao-Feng Zhang, Zeng-Feng Zhang, Pan-Dong Li, Ping-Hua Qu
Summary: This study characterized the chromosome and plasmid sequences, and determined the transferability of plasmids in carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae isolates from the same patient in China. Both isolates exhibited multidrug resistance, with A. baumannii resistant to nine classes of antimicrobials and K. pneumoniae carrying bla(KPC-2) in a plasmid. The study highlights the urgent need to prevent and control co-infections caused by multiple carbapenem-resistant pathogens in the same patient.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Immunology
Dongmei Lv, Yan Zuo, Yuerong Wang, Zhongxin Wang, Yuanhong Xu
Summary: This study analyzed the predictors of occurrence and 30-day mortality for co-infection with carbapenem-resistant Klebsiella pneumoniae (CRKP) and carbapenem-resistant Acinetobacter baumannii (CRAB). Fiberoptic bronchoscopy, repeat transfusions, and exposure to tigecycline were identified as independent risk factors for co-infection. Neutrophil and C-reactive protein levels were found to be associated with 30-day mortality, and their combination could predict mortality.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lucie Semenec, Amy K. K. Cain, Catherine J. J. Dawson, Qi Liu, Hue Dinh, Hannah Lott, Anahit Penesyan, Ram Maharjan, Francesca L. L. Short, Karl A. A. Hassan, Ian T. T. Paulsen
Summary: This study characterizes the genomes of A. baumannii and K. pneumoniae strains co-isolated from a single human lung infection, and investigates their interactions through various assays. The findings shed light on their effects on antimicrobial resistance and virulence during co-infection.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Jose Cedano, Michelle Baez, Fernando Pasteran, Sabrina Daiana Montana, Grace Ra, Venjaminne Fua, Alejandra Corso, Marcelo E. Tolmasky, Robert A. Bonomo, Maria Soledad Ramirez
Summary: This study tested the efficacy of the combination of sulbactam and a novel DBO, zidebactam, against carbapenem-resistant A. baumannii isolates. The results showed that the sulbactam/zidebactam combination was able to restore sulbactam susceptibility in resistant isolates, including those resistant to the sulbactam/avibactam combination.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Amanda Vivo, Margaret A. Fitzpatrick, Katie J. Suda, Makoto M. Jones, Eli N. Perencevich, Michael A. Rubin, Swetha Ramanathan, Geneva M. Wilson, Martin E. Evans, Charlesnika T. Evans
Summary: This study investigated the epidemiology of CRAB and CRPA in veterans and found that positive blood cultures and more comorbidities were associated with higher odds of mortality. These findings can guide clinicians to treat patients with these infections in a timely manner to improve outcomes.
BMC INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Samy Selim, Osama Ahmed Faried, Mohamed S. Almuhayawi, Osama A. Mohammed, Fayez M. Saleh, Mona Warrad
Summary: Acinetobacter baumannii is a nonfermenting Gram-negative bacterium associated with nosocomial infections. This study identified four distinct lineages of A. baumannii and found that genotype (A) isolates showed reduced effectiveness to certain antibiotics. Additionally, the binding affinity of imipenem towards Acinetobacter baumannii OXA beta-lactamase enzymes was investigated.
Article
Infectious Diseases
Massimiliano De Angelis, Maria T. Mascellino, Maria C. Miele, Dania Al Ismail, Marisa Colone, Annarita Stringaro, Vincenzo Vullo, Mario Venditti, Claudio M. Mastroianni, Alessandra Oliva
Summary: This study aimed to evaluate the in vitro activity of N-acetylcysteine (NAC) alone or in combination with beta-lactams against carbapenem-resistant Klebsiella pneumoniae (CR-Kp) and Acinetobacter baumannii (CR-Ab). The results showed that NAC had high in vitro activity against CR-Kp and CR-Ab and enhanced the susceptibility of beta-lactams in the tested strains.
Article
Microbiology
Jingchun Kong, Yue Wang, Zhuocheng Yao, Yishuai Lin, Yi Zhang, Yijia Han, Tieli Zhou, Jianzhong Ye, Jianming Cao
Summary: In this study, the combination of colistin and eugenol was found to have a significant synergistic antibacterial effect against colistin-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae. The combination increased membrane permeability and caused membrane damage, inhibiting bacteria synergistically. Eugenol was non-toxic to cells and the combination was effective in vivo. These findings suggest that the colistin/eugenol combination may be a viable treatment option for colistin-resistant bacterial infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jingchun Kong, Yue Wang, Zhuocheng Yao, Yishuai Lin, Yi Zhang, Yijia Han, Tieli Zhou, Jianzhong Ye, Jianming Cao
Summary: This study found that the combination of colistin and eugenol has a significant synergistic effect in treating colistin-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae infections, reversing their sensitivity to colistin. Eugenol enhances membrane permeability and causes membrane damage, leading to inhibition of bacteria. The combination of colistin and eugenol is also effective in vivo. These findings suggest that the colistin/eugenol combination may be a viable treatment option for clinical infections caused by colistin-resistant pathogens.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Reem M. Hassan, Sherifa T. Salem, Saly Ismail Mostafa Hassan, Asmaa Sayed Hegab, Yasmine S. Elkholy
Summary: This study found that the most prevalent types of carbapenemase genes among CR-AB isolates collected from Egyptian patients were blaOXA-23, followed by blaNDM-1 and blaKPC.
Article
Infectious Diseases
Rebeca Carvalho Lacerda Garcia, Rodrigo Douglas Rodrigues, Ester Carvalho Lacerda Garcia, Maria Helena Rigatto
Summary: This study evaluated the impact of polymyxin B versus colistin on mortality and nephrotoxicity in carbapenem-resistant Acinetobacter baumannii and Pseudomonas aeruginosa bloodstream infections. The results showed that treatment with polymyxin B or colistin did not affect the 30-day mortality in patients with these bacterial infections.
Article
Biotechnology & Applied Microbiology
Yasir Adil Jabbar Alabdali
Summary: The goal of this study was to characterize and detect class 1, 2, and 3 integrons in antibiotic-resistant A. baumannii strains collected from a clinical environment in Iraq. The presence of class 1 integrons was found to significantly contribute to the acquisition of multi-drug resistant phenotypes in A. baumannii isolates, and it can be used as a predictive biomarker for multi-drug resistance.
JOURNAL OF ANTIBIOTICS
(2022)
Article
Biochemistry & Molecular Biology
Arnon Chukamnerd, Kamonnut Singkhamanan, Virasakdi Chongsuvivatwong, Prasit Palittapongarnpim, Yohei Doi, Rattanaruji Pomwised, Chanida Sakunrang, Kongpop Jeenkeawpiam, Mingkwan Yingkajorn, Sarunyou Chusri, Komwit Surachat
Summary: This study conducted whole-genome sequencing to understand the molecular epidemiology of carbapenem-resistant Acinetobacter baumannii (CRAB) in Southern Thailand. The most prevalent sequence type (ST) was ST2 and almost all isolates carried the bla(OXA-23) gene. Various antimicrobial resistance genes, mobile genetic elements, and virulence genes were identified. These findings provide crucial information for the control and treatment of CRAB infections.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Ruohan Wei, Xuemei Yang, Han Liu, Tongyao Wei, Sheng Chen, Xuechen Li
Summary: The study demonstrates that chemically synthesized Pse-CRM197 conjugates can stimulate high immune responses and provide a new alternative for controlling infections caused by multidrug-resistant A. baumannii.
ACS CENTRAL SCIENCE
(2021)
Article
Microbiology
Jinfeng Bao, Lu Xie, Yating Ma, Ran An, Bing Gu, Chengbin Wang
Summary: This study investigates the changes in cefiderocol resistant Klebsiella pneumoniae using proteomic and transcriptomic analyses. The results show that cefiderocol resistance leads to a decrease in biofilm formation ability, which may be associated with the downregulation of related genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kai-Xuan Wang, Chen Yan, Xu Yang, Pei-Yan Zhu, Wen-Wen Cui, Cong Ye, Kan Hu, Ting Lan, Lin-Yan Huang, Wan Wang, Ping Ma, Su-Hua Qi, Bing Gu, Lan Luo
Summary: In this study, we found that autophagy-mediated Sirt1 downregulation may be a promising therapeutic target for prostate cancer.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biophysics
Yanna Shao, Wenxing Xu, Yin Zheng, Juan Wang, Jihang Xie, Zhenjun Zhu, Xinran Xiang, Qinghua Ye, Youxiong Zhang, Liang Xue, Bing Gu, Jierong Chen, Jumei Zhang, Qingping Wu, Yu Ding
Summary: This study proposes a novel method of polyallylamine hydrochloride (PAH)-mediated metal growth for the detection of Escherichia coli O157:H7 by using AuNP-ICA. The method achieves highly enhanced colorimetric signals by controlling the growth of Cu shells on the AuNP core using PAH as the growth framework. The introduction of PAH eliminates non-specific adsorption and improves the signal-to-noise ratio for the detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Immunology
Xiaoxiao Wu, Bing Gu, Huan Yang
Summary: The intestinal mucosa is a crucial structure for defending against pathogen infection, comprising four barriers that serve different but interconnected functions. Pathogenic bacteria have the ability to damage these barriers; however, γδT cells play a significant role in regulating and monitoring this process. Commensal bacteria are involved in the activation and migration of γδT cells to resist pathogen invasion. It has been found that the migration pattern of γδT cells is altered during pathogen invasion, leading to accelerated pathogen clearance and limited opportunistic invasion by commensal bacteria, thus maintaining intestinal health.
INTERNATIONAL REVIEWS OF IMMUNOLOGY
(2023)
Article
Integrative & Complementary Medicine
Hansen Chen, Yunxia Luo, Bun Tsoi, Bing Gu, Suhua Qi, Jiangang Shen
Summary: This study aimed to investigate the potential of a traditional Chinese medicinal formula, AGNHW, as an adjuvant therapy with t-PA in ischemic stroke treatment. It was found that AGNHW could protect the integrity of the blood-brain barrier, reduce hemorrhagic transformation, and improve therapeutic outcomes by inhibiting peroxynitrite-mediated MMP-9 activation.
Article
Immunology
Jianing Yang, Xuejiao Hu, Wenzhuo Wang, Yujing Yang, Xinqiang Zhang, Wei Fang, Lei Zhang, Shan Li, Bing Gu
Summary: A genotyping RT-LAMP method was designed and validated to specifically detect the Delta variant. By analyzing the amplification efficiency of two sets of primers, a Cq ratio-based RT-LAMP method was developed, which showed 100% accuracy in detecting SARS-CoV-2 clinical samples. The method demonstrated a good ability to distinguish between Delta and non-Delta variants. The study suggests that this rapid, accurate, and cost-effective RT-LAMP method may facilitate COVID-19 surveillance.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Infectious Diseases
Lifang Sun, Yunju Xiao, Weilong Huang, Jianwei Lai, Jingwen Lyu, Bingjun Ye, Hongyu Chen, Bing Gu
Summary: This study investigated the incidence, molecular epidemiology, and antimicrobial resistance of group A Streptococcus (GAS) isolates in paediatric patients at Shenzhen Children's Hospital from 2016 to 2020. The results showed a high incidence of GAS infections, with high resistance to erythromycin and clindamycin but sensitivity to penicillin. The common emm types were emm12 and emm1.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Cardiac & Cardiovascular Systems
Wen-Wen Cui, Cong Ye, Kai-Xuan Wang, Xu Yang, Pei-Yan Zhu, Kan Hu, Ting Lan, Lin-Yan Huang, Wan Wang, Bing Gu, Chen Yan, Ping Ma, Su-Hua Qi, Lan Luo
Summary: This study found that Momordica.charantia-derived EVs-like nanovesicles (MCELNs) can protect cardiomyocytes from radiation-induced damage and have antioxidant effects. MCELNs promote cell proliferation, alleviate cell apoptosis and DNA damage, and protect mitochondrial function. Moreover, MCELNs can restore the phosphorylation levels of ROS-related proteins and mitigate myocardial injury and fibrosis caused by thoracic radiation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Bing-Kang Sun, Rui-Yu Wang, Bei Li, Xing Fan, Yuan Zhou, Bing Gu, Yang-Yang Yan
Summary: A rapid and accurate analytical method was established to identify CREC and CSEC strains by detecting polypeptides and analyzing MS data with pattern recognition analyses. OPLS-DA exhibited the highest accuracy in predicting these strains.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Nanoscience & Nanotechnology
Feng Gu, Shan Hu, Yunjian Wu, Changyu Wu, Ying Yang, Bing Gu, Hong Du
Summary: This study successfully detected the drug resistance of non-Candida albicans using SERS technology combined with OPLS-DA analysis. The method is non-destructive to the cell wall and easy to operate, showing promising clinical application potential.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Cell Biology
Jierong Chen, Lixue Cao, Jianhong Ma, Caifeng Yue, Dandan Zhu, Ran An, Xiaoxiao Wang, Yunmiao Guo, Bing Gu
Summary: This study reveals the critical role of the HDAC8/IRF1/SUCNR1 axis in colorectal cancer (CRC), with implications in cell growth, autophagy, and liver metastasis.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Infectious Diseases
Jingwen Lyu, Qianyun Deng, Rongqing Li, Benshun Tian, Yunhu Zhao, Xuejiao Hu, Maohua Zhou, Bing Gu
Summary: This case report highlights the importance of combining metagenomic next-generation sequencing (mNGS) with traditional tests in the clinical diagnosis of mixed lung infections in infants without HIV infection. mNGS is a new method that can accurately identify multiple causes of pneumonia.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Biophysics
Chongwen Wang, Chaoguang Wang, Jiaxuan Li, Zhijie Tu, Bing Gu, Shengqi Wang
Summary: Researchers have developed a bi-channel surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFA) technique using nanostickers for sensitive and multiplexed detection of bacteria. The results showed that the nanostickers could rapidly detect multiple pathogens with high sensitivity and multiplex analysis capability.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Immunology
Yiqing Tian, Jian Fang, Fanpeng Zeng, Yongqiang Chen, Yunfeng Pei, Feng Gu, Chen Ding, Guoping Niu, Bing Gu
Summary: This article describes the role of mesenchymal stem cells in tumors and the impact of hypoxic environments on tumor development. It discusses the mutual influence between hypoxic MSCs and immune cells in the TME, as well as the potential drug resistance and therapeutic effects of hypoxic MSCs.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Liang Wang, Xue-Di Zhang, Jia-Wei Tang, Zhang-Wen Ma, Muhammad Usman, Qing-Hua Liu, Chang-Yu Wu, Fen Li, Zuo-Bin Zhu, Bing Gu
Summary: This study utilized deep learning algorithms to analyze spectra generated by a handheld Raman spectrometer, successfully distinguishing sputum samples with or without Mtb strains, separating pulmonary and extra-pulmonary Mtb strains, and effectively distinguishing Mtb strains with different drug-resistant profiles.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)