Article
Microbiology
Hisayuki Komaki
Summary: This study aimed to clarify the taxonomic relationships among Streptomyces costaricanus, Streptomyces murinus and Streptomyces phaeogriseichromatogenes using multilocus sequence analysis and bioinformatics methods, revealing that these three strains should be classified as the same species. The presence of a similar set of secondary metabolite-biosynthetic gene clusters for polyketides and nonribosomal peptides further supported their similarity. Therefore, S. costaricanus and S. phaeogriseichromatogenes should be reclassified as later heterotypic synonyms of S. murinus.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Julie B. Nielsen, Tetiana Gren, Omkar S. Mohite, Tue S. Jorgensen, Andreas K. Klitgaard, Anna-Sophie Mourched, Kai Blin, Daniel Oves-Costales, Olga Genilloud, Thomas O. Larsen, David Tanner, Tilmann Weber, Charlotte H. Gotfredsen, Pep Charusanti
Summary: Actinomycetes produce a wealth of complex natural products. Researchers have identified the biosynthetic gene cluster for pyracrimycin A and proposed a complete biosynthesis pathway based on feeding experiments and isotope labeling analysis.
ACS CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Bo Pang, Rijing Liao, Zhijun Tang, Shengjie Guo, Zhuhua Wu, Wen Liu
Summary: The study elucidates the biosynthetic pathways of caerulomycins and collismycins, validates the generality of 2,2'-bipyridine formation, and clarifies the process for 2,2'-bipyridine furcation.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Hisayuki Komaki, Tomohiko Tamura
Summary: Streptomyces cellostaticus is a potential source of diverse polyketides and nonribosomal peptides, as revealed by the sequencing of its whole genome and survey of PKS and NRPS gene clusters. The genome of S. cellostaticus encoded multiple PKS and NRPS gene clusters, with annotated products including known and unknown secondary metabolites.
FERMENTATION-BASEL
(2023)
Article
Plant Sciences
Han Wang, Huan Qi, Hui Zhang, Shao-Yong Zhang, Cheng-Hong Zhang, Li -Qin Zhang, Wen-Sheng Xiang, Ji-Dong Wang
Summary: Bioinformatics analysis and HPLC-DAD analysis of a whole genome sequence revealed the ability of Streptomyces sp. Hu103 to produce skyllamycin analogues. Chemical investigation of this strain led to the discovery of four new cinnamoyl-containing cyclo-peptides, anulamycins A-D (1-4), two new cinnamoyl-containing linear peptides, anulamycins E and F (5 and 6), and two known cyclopeptides, skyllamycins A (7) and B (8). Their structures and absolute configurations were determined by detailed analysis of NMR and HRESIMS/MS spectroscopic data and the advanced Marfey's method. Compounds 1-4 exhibited antibacterial activity comparable to skyllamycins A and B.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Chemistry, Medicinal
Edna M. Sabido, Chuckcris P. Tenebro, Dana Joanne Von L. Trono, Carmela Vannette B. Vicera, Sheeny Fane L. Leonida, Jose Jeffrey Wayne B. Maybay, Rikka Reyes-Salarda, Diana S. Amago, Angelica Marie Aguadera, May C. Octaviano, Jonel P. Saludes, Doralyn S. Dalisay
Summary: This study explored the biodiversity, bioactivities, and metabolite variation of culturable actinomycetes isolated from marine sediments in the Visayan Sea, Philippines. The research revealed that the chemical diversity among these strains influences the variation observed in their biological activities, with taxonomically identical strains producing core and strain-specific metabolites. The study highlights the potential of marine-derived Streptomyces as a source of small molecules against ESKAPE pathogens and cancer, and the unique strain-specific secondary metabolites produced by Streptomyces species strains offer new chemical space for natural product discovery.
Article
Ecology
Laima Lukoseviciute, Jolanta Lebedeva, Nomeda Kuisiene
Summary: The study aimed to identify and characterize genes responsible for the production of secondary metabolites in the microbial community of one of the deepest caves in the world. Taxonomic diversity analysis showed that most PKS sequences were attributed to Actinobacteria, while NRPS sequences were more diverse. Only three putative metabolites were predicted, indicating the potential for novel secondary metabolites production.
Article
Biochemistry & Molecular Biology
Madhaiyan Munusamy, Kenneth Tan, Choy Eng Nge, Martin Muthee Gakuubi, Sharon Crasta, Yoganathan Kanagasundaram, Siew Bee Ng
Summary: In this study, 30 Ascomycetes fungi were investigated for their biosynthetic potential and ability to produce antimicrobial secondary metabolites. Screening led to the identification of PKSs and NRPSs genes, suggesting the conserved biosynthesis of closely related compounds from different fungi. Seven compounds were isolated, including Vioxanthin with the best antibacterial activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Peng Hou, Vincent V. Nowak, Chanel J. Taylor, Mark J. Calcott, Allison Knight, Jeremy G. Owen
Summary: In this study, 322 actinomycetes were isolated and their genome sequences were obtained from New Zealand lichens. By enumerating biosynthetic gene clusters (BGCs) and comparing to reference collections, it was found that actinomycetes from New Zealand lichens have the genetic capacity to produce large numbers of natural products, which are expected to be different from those identified in previous soil-based studies. This study sheds light on the actinomycete assemblage in New Zealand lichens and highlights the potential of lichen-sourced actinobacteria for discovering new secondary metabolites.
Review
Chemistry, Physical
Maria L. Adrover-Castellano, Jennifer J. Schmidt, David H. Sherman
Summary: This review explores the general cyclization mechanism of TE domain in NRPS and PKS, including oligomerization and CLCs. It also discusses other cyclization catalysts such as C-T domains, R domains, R-D domains, Dieckmann cyclases, among others. The diversity of cyclization catalysts highlighted in the studies for the production of biologically relevant, complex cyclic natural products and related compounds is summarized here.
Review
Biochemistry & Molecular Biology
Yongwei Zhao, Y. T. Candace Ho, Julien Tailhades, Max Cryle
Summary: Researchers are intrigued by the complex biosynthesis of glycopeptide antibiotics, particularly the role of the X-domain in recruiting P450 enzymes. In vitro studies have provided insights into the tolerances and limitations of the GPA cyclisation cascade, paving the way for future reengineering of this important antibiotic class.
Article
Microbiology
Soren Planckaert, Benoit Deflandre, Anne-Mare De Vries, Maarten Ameye, Jose C. Martins, Kris Audenaert, Sebastien Rigali, Bart Devreese
Summary: The discovery of new rotihibin analogues in Streptomyces scabies, a major pathogen causing common scab disease, provides insights into their herbicidal activity and potential biotechnological production. Through genomic analysis, a cryptic biosynthetic gene cluster was identified, leading to a better understanding of the mechanism behind targeting the plant TORK pathway. The existence of other variants in plant-associated Streptomyces strains raises new questions about the function of this lipopeptide.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biotechnology & Applied Microbiology
Rocky Chau, Leanne A. Pearson, Jesse Cain, John A. Kalaitzis, Brett A. Neilan
Summary: This study highlights the biosynthetic potential of Pseudoalteromonas strain HM-SA03, capable of producing alterochromides and potentially six other specialized metabolites. Through genomic analysis, a novel siderophore biosynthesis gene cluster with unprecedented architecture was identified in the HM-SA03 genome. Phylogenetic analysis revealed a subset of strains with extraordinary biosynthetic potential within the genus Pseudoalteromonas.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Jinsheng Cui, Eunji Kim, Dong Hyun Moon, Tae Ho Kim, Ilnam Kang, Yeonjung Lim, Daniel Shin, Sunghoon Hwang, Young Eun Du, Myoung Chong Song, Munhyung Bae, Jang-Cheon Cho, Jichan Jang, Sang Kook Lee, Yeo Joon Yoon, Dong-Chan Oh
Summary: In this study, two new lipo-decapeptides, taeanamides A and B, were discovered from a Gram-positive bacterium isolated from a mudflat sample in Korea. The structures and configurations of the compounds were determined using mass spectrometry, NMR spectroscopy, and bioinformatics. Taeanamide A showed mild anti-tuberculosis activity, while taeanamide B exhibited significant bioactivity against several cancer cell lines.
Article
Biochemistry & Molecular Biology
Justin C. Miller, Allison J. Hollatz, Mary A. Schuler
Summary: The study identifies differences in the terpene indole alkaloid (TIA) pathways in Catharanthus roseus and Camptotheca acuminata, and demonstrates the ability of specific P450 proteins to convert certain compounds in these pathways. The research provides valuable tools for further examination of substrate recognition and the unique mechanism of C-C bond scission involved in these pathways.
Article
Biochemistry & Molecular Biology
R. Conde, R. Laires, L. G. Goncalves, A. Rizvi, C. Barroso, M. Villar, R. Macedo, M. J. Simoes, S. Gaddam, P. Lamosa, L. Puchades-Carrasco, A. Pineda-Lucena, A. B. Patel, S. C. Mande, S. Banerjee, M. Matzapetakis, A. Coelho
Summary: This study utilized Nuclear Magnetic Resonance based metabolomics to discover a signature of biomarkers for tuberculosis diagnosis. Six metabolites associated with TB infection were validated, in line with the recommendations of the World Health Organization.
BIOMEDICAL JOURNAL
(2022)
Article
Oncology
Borja Ruiz-Fernandez de Cordoba, Haritz Moreno, Karmele Valencia, Naiara Perurena, Pablo Ruedas, Thomas Walle, Alberto Pezonaga-Torres, Juan Hinojosa, Elisabet Guruceaga, Antonio Pineda-Lucena, Marta Abengozar-Muela, Denis Cochonneau, Carolina Zandueta, Susana Martinez-Canarias, Alvaro Teijeira, Daniel Ajona, Sergio Ortiz-Espinosa, Xabier Morales, Carlos Ortiz de Solorzano, Marta Santisteban, Luis Ramos-Garcia, Laura Guembe, Vratislav Strnad, Dominique Heymann, Sandra Hervas-Stubbs, Ruben Pio, Maria E. Rodriguez-Ruiz, Carlos E. de Andrea, Silvestre Vicent, Ignacio Melero, Fernando Lecanda, Rafael Martinez-Monge
Summary: Locoregional failure in breast cancer patients after surgery and irradiation is linked to a poor prognosis. The ENPP1/HP axis is exploited by tumor cells to promote local recurrence by suppressing immune cells. Blocking this axis offers new opportunities for therapeutic intervention.
Review
Oncology
Nuria Gomez-Cebrian, Jose Luis Poveda, Antonio Pineda-Lucena, Leonor Puchades-Carrasco
Summary: Prostate cancer is a heterogeneous tumor with a highly variable clinical outcome. Integrating multi-omics datasets can be a powerful approach for developing novel metabolic signatures to improve the clinical management of prostate cancer patients.
Article
Cell Biology
Cristina Solana-Manrique, Francisco Jose Sanz, Isabel Torregrosa, Martina Palomino-Schatzlein, Carolina Hernandez-Oliver, Antonio Pineda-Lucena, Nuria Paricio
Summary: The study investigates metabolic disturbances underlying the pathogenesis of Parkinson's disease (PD) using a Drosophila PD model. Through metabolomic analysis, the study finds that metabolic alterations are associated with PD and could potentially serve as therapeutic targets and biomarkers for the disease.
Article
Biochemistry & Molecular Biology
Jose L. Neira, Martina Palomino-Schatzlein
Summary: The phosphotransferase system (PTS), consisting of five proteins, regulates the use of sugars in bacteria. The second protein in the chain, HPr, plays a role in bacterial carbon sources utilization and can be phosphorylated at Ser46. Additionally, two other proteins, Rsd and HPrIVP, bind to HPr at His15. Fragments of HPr were designed and shown to be mainly disordered but still capable of binding to their respective protein partners. Binding was related to the presence of a weak native-like structure around His15.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Environmental Sciences
Marina Moreira Freire, Cristina Gomez, Josino Costa Moreira, Ana Rosa Linde Arias
Summary: This study evaluates the impact of contamination in Brazil's Guanabara Bay on two fish species using a multibiomarker approach. The results indicate that fish in the bay are greatly affected by environmental pollution, specifically heavy metal and polycyclic aromatic hydrocarbon exposure.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2023)
Article
Biotechnology & Applied Microbiology
Putty Ekadewi, Rita Arbianti, Cristina Gomez, Tania Surya Utami
Summary: This study explores the use of a designed microbial community to produce biohydrogen in single-chamber microbial electrolysis cells (MECs). By using a designed consortium, the competition of metabolic pathways can be avoided. The MECs inoculated with the designed consortium showed better hydrogen production performance compared to those inoculated with a natural soil consortium.
FOOD TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Medicine, General & Internal
Jose Raul Herance, Andreea Ciudin, Ruben Lamas-Domingo, Carolina Aparicio-Gomez, Cristina Hernandez, Rafael Simo, Martina Palomino-Schatzlein
Summary: The prevalence of diabetes type 1 (T1D) is increasing worldwide. This study aimed to find new biomarkers of T1D in red blood cells (RBCs), which have not been explored before. The metabolomic and lipidomic profiles of RBCs from T1D patients and healthy controls were analyzed using NMR spectroscopy. Significant changes were observed in amino acids, organic acids, creatine, phosphocreatine, lipid length, and choline derivatives. These changes indicate alterations in glycolysis, BCAA metabolism, and phospholipid metabolism. The study concluded that the metabolic and lipidomic profile of RBCs can be used as biomarker candidates for the earlier diagnosis and monitoring of T1D patients.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Suhui Ye, Brian Molloy, Ignacio Perez-Victoria, Ignacio Montero, Alfredo F. Brana, Carlos Olano, Sonia Arca, Jesus Martin, Fernando Reyes, Jose A. Salas, Carmen Mendez
Summary: Genome mining using bioinformatics tools allowed for uncovering hidden biosynthesis gene clusters, while an alternative approach involving the analysis of Streptomyces Antibiotic Regulatory Proteins (SARP) encoding genes was used to identify cryptic gene clusters. This strategy led to the discovery of the new ahb cluster in Streptomyces argillaceus and the identification of ahbamycins as a new family of metabolites with antibiotic and antitumor activity. Further investigation revealed a hybrid compound, AHB18, produced through metabolic crosstalk between the ahb and argimycin P pathways. This finding opens up possibilities for generating new compounds through combinatorial biosynthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Josefa Tolosa, Eva Serrano Candelas, Jose Luis Valles Pardo, Addel Goya, Salvador Moncho, Rafael Gozalbes, Martina Palomino Schaetzlein
Summary: Mycotoxins, which are produced by fungi, pose a threat to public health due to their potential carcinogenic, mutagenic, and teratogenic effects. This study developed a comprehensive database of 4360 mycotoxins classified into 170 categories. The use of QSAR models allowed for accurate and reliable prediction of mutagenicity, genotoxicity, and carcinogenicity, meeting regulatory criteria. The integrated web server provides a valuable tool for exploring the mycotoxin database and predicting toxicity.
Article
Microbiology
Lorena Cuervo, Monica G. Malmierca, Raul Garcia-Salcedo, Carmen Mendez, Jose A. Salas, Carlos Olano, Ana Ceniceros
Summary: The search for novel bioactive compounds to overcome resistance to current therapeutics has become crucial. Streptomyces spp. are a major source of bioactive compounds used in medicine. In this study, global transcriptional regulators and housekeeping genes known to enhance secondary metabolite production in Streptomyces coelicolor were cloned and expressed in different strains. The recombinant plasmids were also inserted into Streptomyces strains with enhanced secondary metabolism. Media with varied carbon and nitrogen sources were used, and the strains' metabolite production was analyzed. The study demonstrated the overproduction and activation of known metabolites and the potential for manipulating Streptomyces metabolism and exploring their wide range of secondary metabolites.
Article
Biochemistry & Molecular Biology
Ana Ceniceros, Librada Canedo, Carmen Mendez, Carlos Olano, Carmen Schleissner, Carmen Cuevas, Fernando de la Calle, Jose A. Salas
Summary: Three novel lipopeptides were isolated from a marine sediment-derived Streptomyces tuirus and they exhibited cytotoxic activity against human cancer cell lines. The biosynthesis of these lipopeptides was found to be dependent on a gene cluster.
Article
Microbiology
Celia Bautista-Crescencio, Arturo Casimiro-Ramos, M. Jonathan Fragoso-Vazquez, Jose Correa-Basurto, Carlos Olano, Cesar Hernandez-Rodriguez, Lourdes Villa-Tanaca
Summary: There is an urgent need to develop new antifungals due to the increasing prevalence of multidrug-resistant fungal infections and the recent emergence of COVID-19-associated candidiasis. In this study, antifungal metabolites produced by Streptomyces albidoflavus Q were investigated, and two secondary metabolites were found to have a good inhibitory effect on Candida glabrata, which may be potential candidates for treating fungal infections.
MICROBIOLOGY SPECTRUM
(2023)
