Journal
JOURNAL OF ANTIBIOTICS
Volume 64, Issue 1, Pages 97-101Publisher
JAPAN ANTIBIOTICS RESEARCH ASSOC
DOI: 10.1038/ja.2010.148
Keywords
actinorhodin; protein phosphorylation; S-adenosylmethionine; Streptomyces coelicolor; undecylprodigiosin
Funding
- 21C Frontier Microbial Genomics and Application Center program [11-2008-16-001-00]
- Korean Ministry of Science and Technology
- Korean government (MEST) [2009-0071562]
- Korean Government (MOEHRD) [KRF-2007-211-000039]
- Brain Korea 21 (BK21)
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Streptomycetes are the major natural source of clinical antibiotics. The enhanced secondary metabolite production of many streptomycetes by S-adenosylmethionine (SAM) in previous studies suggested the existence of a common SAM regulatory effect. We screened nine proteins using the phosphoprotein purification column from Streptomyces coelicolor. Among them, genes (SCO5477, SCO5113, SCO4647, SCO4885 and SCO1793) for five proteins were disrupted by insertion mutation. The undecylprodigiosin and actinorhodin productions were changed in all mutations. The SAM-induced enhancement of actinorhodin production was abolished by all mutations except SCO4885 mutation, which reduced the production of actinorhodin and undecylprodigiosin with SAM treatment. This study demonstrates that phosphoprotein affinity purification can be used as a screening method to identify the proteins involved SAM signaling. The Journal of Antibiotics (2011) 64, 97-101; doi:10.1038/ja.2010.148; published online 8 December 2010
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