Review
Veterinary Sciences
Souvik Ghosh, Yashpal S. Malik
Summary: Picobirnaviruses are bisegmented double-stranded RNA viruses detected in a wide range of animal species and environmental samples. There is ongoing debate about the true host of these viruses.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Sylvia Dixon, Di Evans, Thinza Vindevoghel, Michael P. Ward, Anne Quain
Summary: This study evaluated video footage of calf roping events from two rodeos and found that calves showed signs of distress and fear in all phases of the event. These findings contribute to discussions on the welfare of calves used in rodeos.
Article
Chemistry, Applied
Marefa Jahan, Peter C. Thomson, Peter C. Wynn, Bing Wang
Summary: The study revealed that the concentration of Neu5Gc in red meat varies depending on tissue and species, with some animals lacking Neu5Gc in muscle and organ tissues. These findings provide consumers with guidance on animal meat preferences and shed light on the functionality of Neu5Gc.
Article
Immunology
Markus G. Klammer, Omar Dzaye, Thomas Wallach, Christina Krueger, Dorothea Gaessler, Alice Buonfiglioli, Katja Derkow, Helmut Kettenmann, Melanie M. Brinkmann, Seija Lehnardt
Summary: UNC93B1, a chaperone protein, is widely expressed in the murine brain and modulates neuroinflammation and neuronal injury triggered by extracellular microRNA and subsequent TLR signaling.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
David Dora, Imre Voros, Zoltan V. Varga, Peter Takacs, Vanda Teglasi, Judit Moldvay, Zoltan Lohinai
Summary: The expression of BRAF RNA in lung adenocarcinoma is increased and associated with tumor necrosis, immune checkpoint biology, and outcomes. Further research on targeting the overexpressed BRAF pathway in lung adenocarcinoma is recommended.
TRANSLATIONAL LUNG CANCER RESEARCH
(2023)
Article
Agriculture, Multidisciplinary
Yue Shi, Binsong Han, Lina Zhang, Peng Zhou
Summary: Human and camel milk contain higher concentrations of OSs compared to bovine, caprine, and ovine milk. Camel milk is more similar to human milk in terms of OSs. The study provides insights into the potential applications of OSs in medical and functional foods based on the characteristics and concentrations of OSs in milk from different species.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Cell Biology
Sonia Nasi, Mariela Castelblanco, Veronique Chobaz, Driss Ehirchiou, Alexander So, Ilaria Bernabei, Teruo Kusano, Takeshi Nishino, Ken Okamoto, Nathalie Busso
Summary: Pathologic calcification of cartilage is associated with aging and cartilage injury, potentially accelerating osteoarthritis. Xanthine oxidoreductase (XOR), particularly its XO form, may contribute to chondrocyte mineralization and pathological calcification in osteoarthritis cartilage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Ayumi Saito, Tadaaki Nishikawa, Hiroshi Yoshida, Chiharu Mizoguchi, Rui Kitadai, Kasumi Yamamoto, Shu Yazaki, Yuki Kojima, Mitsuya Ishikawa, Tomoyasu Kato, Kan Yonemori
Summary: This study investigated the expression and clinical significance of folate receptor alpha (FRα) in uterine carcinosarcoma (UCS) and ovarian carcinosarcoma (OCS). The results showed that FRα was universally expressed in all patients, but it was not significantly associated with clinicopathological features or prognosis. However, FRα-targeted therapies may have potential therapeutic effects for gynecologic carcinosarcoma.
GYNECOLOGIC ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nicolas Mouchet, Nicolas Vu, Bruno Turlin, Nathalie Rioux-Leclercq, Stephane Jouneau, Michel Samson, Laurence Amiot
Summary: The study found that HLA-G(+) mast cells could be observed in fibrotic regions of the liver, lungs, and kidneys. More than half of the HLA-G(+) cells in fibrotic areas were mast cells. In some cases, B lymphocytes expressing HLA-G were observed, possibly reinforcing their antifibrotic role.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jessica A. G. Johnson, Hongxia Liu, Ulli Hoger, Samantha M. Rogers, Kajanan Sivapalan, Andrew S. French, Paivi H. Torkkeli
Summary: Mechanosensory neurons in spiders predominantly express the Piezo protein as the MET channel, with no significant detection of other MET channel families. The Piezo protein is widely expressed in the spider nervous system, potentially contributing to mechanosensory transduction in mechanosensilla and serving other functions in peripheral and central neurons. Blocking MET channels did not affect mechanically activated currents in VS-3 neurons, but reduced action potential firing under electrical stimulation.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Moe Sakakibara, Hayakazu Sumida, Keisuke Yanagida, Sosuke Miyasato, Motonao Nakamura, Shinichi Sato
Summary: T2R38 gene expression is higher in the lesional skin of atopic dermatitis (AD) patients and is correlated with markers of inflammation. T2R38 receptors are also expressed in T cells infiltrating the AD lesional skin. Moreover, T2R38 agonists inhibit cell migration, with potential endogenous ligands for T2R38 present in skin tissue extracts and supernatants.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Stefania Pianetti, Kathy D. Miller, Hannah H. H. Chen, Sandra Althouse, Sha Cao, Steven J. Michael, Gail E. Sonenshein, Nora D. Mineva
Summary: Breast malignancies are the leading cause of cancer-related death in women. ADAM8 has been identified as a marker of poor survival and a driver of Triple-Negative Breast Cancer growth. A highly specific IHC assay for detecting ADAM8-positive breast tumors has been developed.
CANCER CELL INTERNATIONAL
(2023)
Article
Oncology
Mateja Condic, Eva K. K. Egger, Niklas Kluemper, Glen Kristiansen, Alexander Mustea, Thore Thiesler, Damian J. J. Ralser
Summary: This study investigated the expression of TROP-2 protein in 103 patients with primary vulvar squamous cell carcinoma (VSCC). The results showed that 97.1% of the patients had TROP-2 expression, with 74.8% displaying high expression. Additionally, high TROP-2 expression was associated with favorable clinical outcomes in HPV-independent VSCC.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Veterinary Sciences
Yuan Zhao, Gaowa Naren, Jianan Qiang, Guixin Qin, Nan Bao, Mohammed Hamdy Farouk
Summary: The study identified epitopes of beta-conglycinin that lead to allergy in different animal species, showing that most epitopes exhibit different binding abilities to the three animal sera, with the biggest sensitive regions varying between swine, bovine, and rats.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Jovita Luksaite, Egle Zokaityte, Vytaute Starkute, Sonata Sidlauskiene, Gintare Zokaityte, Elena Bartkiene
Summary: This study aimed to personalize AAT by selecting animal species and breeds based on the emotions induced in individuals by different animal species and breeds. The results showed that both animal species and breeds significantly influence the intensity and valence of emotions, particularly 'neutral' and 'happy', providing a personalized strategy for improving AAT and helping individuals select a pet.
Article
Biochemistry & Molecular Biology
Kristina J. H. Kleinwort, Maria Weigand, Lydia Hoffmann, Roxane L. Degroote, Richard Dietrich, Erwin Maertlbauer, Stefanie M. Hauck, Cornelia A. Deeg
Summary: Recently, there have been stability issues with dairy products with extended shelf life, resulting in significant liquefaction. This project identified the enzymes responsible for this liquefaction through proteomic analysis. These findings are useful for adjusting production protocols and additives to extend the shelf life of these products.
Article
Nutrition & Dietetics
Marie-Theres Huemer, Agnese Petrera, Stefanie M. Hauck, Michael Drey, Annette Peters, Barbara Thorand
Summary: This study utilized proteomic profiling to explore the association between the phase angle (PhA) and plasma protein markers. Seven protein markers were identified to be strongly associated with PhA. These markers are involved in regulating cell growth, organ development, and other biological processes.
CLINICAL NUTRITION
(2022)
Article
Multidisciplinary Sciences
Judith Thomas, Maria Fernanda Martinez-Reza, Manja Thorwirth, Yvette Zarb, Karl-Klaus Conzelmann, Stefanie M. Hauck, Sofia Grade, Magdalena Goetz
Summary: This study examines the impact of amyloid pathology and aging on transplantation connectivity and reveals the crucial role of host pathology in shaping the input connectome.
Article
Multidisciplinary Sciences
Sofia Grade, Judith Thomas, Yvette Zarb, Manja Thorwirth, Karl-Klaus Conzelmann, Stefanie M. Hauck, Magdalena Goetz
Summary: The study investigates the performance of transplanted cells for restoring neuronal circuits in the brain under different conditions, highlighting the increased graft inputs in the brain with traumatic injury compared to intact or inflamed conditions.
Article
Biochemistry & Molecular Biology
Anne L. C. Hoffmann, Stefanie M. Hauck, Cornelia A. Deeg, Roxane L. Degroote
Summary: In the pathophysiology of autoimmune-mediated uveitis, granulocytes have been identified as possible disease mediators. Using granulocytes from horses with equine recurrent uveitis (ERU), this study aimed to identify early molecular mechanisms involved in this dysregulated immune response. Differential proteomics analysis revealed significant differences in protein abundance of 170 proteins in ERU, suggesting a divergent reaction profile of pre-activated granulocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Valerie Demais, Anne Pohl, Kirsten A. Wunderlich, Anna M. Pfaller, Lew Kaplan, Amelie Barthelemy, Robin Dittrich, Berta Puig, Bernd Giebel, Stefanie M. Hauck, Frank W. Pfrieger, Antje Grosche
Summary: This study reveals the communication mechanism of Muller cells in the retina of adult mice through the release of extracellular vesicles. The Muller cell-specific SNARE protein VAMP5 is identified as a component of extracellular vesicles and is responsive to ischemia. Furthermore, differences in the secretomes between Muller cells and neurons in vitro are uncovered.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biochemistry & Molecular Biology
Lucia Korbonits, Kristina J. H. Kleinwort, Barbara Amann, Andrea Didier, Erwin Martlbauer, Stefanie M. Hauck, Cornelia A. Deeg
Summary: This study aimed to detect proteomic changes in cows with different MAP infection status after co-incubation with viable MAP in vitro. The results showed that MAP-resistant cows exhibited enriched immune-related signaling pathways, indicating a successful defensive immune response to MAP. On the other hand, persistently MAP-infected cows did not show enrichment in immune-related signaling pathways. These distinct immune responses contribute to a better understanding of the bovine immune response and susceptibility mechanisms to MAP.
Article
Endocrinology & Metabolism
Haifa Maalmi, Alexander Strom, Agnese Petrera, Stefanie M. Hauck, Klaus Strassburger, Oliver Kuss, Oana-Patricia Zaharia, Gidon J. Boenhof, Wolfgang Rathmann, Sandra Trenkamp, Volker Burkart, Julia Szendroedi, Dan Ziegler, Michael Roden, Christian Herder
Summary: No established blood-based biomarker exists for monitoring diabetic sensorimotor polyneuropathy (DSPN) and evaluating treatment response. This study found that higher serum levels of neurofilament light chain (NFL) were associated with prevalent DSPN and nerve dysfunction in individuals recently diagnosed with diabetes. The findings suggest that NFL could be a potential biomarker for DSPN.
Article
Endocrinology & Metabolism
Hong Luo, Alina Bauer, Jana Nano, Agnese Petrera, Wolfgang Rathmann, Christian Herder, Stefanie M. Hauck, Benjamin B. Sun, Annika Hoyer, Annette Peters, Barbara Thorand
Summary: This study aimed to investigate the role of plasma proteins in glucose metabolism and type 2 diabetes development. The study identified several novel proteins associated with glucose metabolism abnormalities and type 2 diabetes, as well as confirming previously reported proteins. These findings highlight the importance of proteins in the pathogenesis of type 2 diabetes and suggest potential targets for diabetes treatment and prevention.
Article
Biochemistry & Molecular Biology
Marie-Christin Schilloks, Isabella-Maria Giese, Arne Hinrichs, Lucia Korbonits, Stefanie M. Hauck, Eckhard Wolf, Cornelia A. Deeg
Summary: This study investigated the effects of impaired GHR signaling on immune functions and immunometabolism in GHR-KO pigs. The results showed significant differences in the relative proportion of the CD4(+)CD8a(-) subpopulation and IFN-a levels between GHR-KO pigs and WT controls. However, no significant difference was found in the respiratory capacity and the capacity for polyclonal stimulation in PBMCs between the two groups. Proteome analysis revealed multiple significant protein abundance differences related to amino acid metabolism, beta-oxidation of fatty acids, insulin secretion signaling, and oxidative phosphorylation between GHR-KO pigs and WT pigs.
Article
Biochemistry & Molecular Biology
Adrian Schmalen, Ilona E. Kammerl, Silke Meiners, Elfriede Noessner, Cornelia A. Deeg, Stefanie M. Hauck
Summary: The majority of MHC class I-presented peptides are a result of proteasomal protein turnover, with inflammation inducing the specialized immunoproteasome for antigenic peptide generation. IFN gamma treatment enhances proteasomal cleavage probabilities for MHC class I ligands and alters the contribution of different HLA allotypes to the immunopeptidome. Specific HLA allotypes, such as HLA-A*30:01, HLA-A*03:01, and HLA-A*11:01, present peptides with reduced C-terminal proteasomal cleavage probabilities, potentially affecting antigen presentation by the proteasome and other cellular proteases.
Article
Biochemical Research Methods
Oliver Kardell, Stephan Breimann, Stefanie M. Hauck
Summary: mpwR is an R package designed for standardized comparison of mass spectrometry-based proteomic label-free workflows. It allows easy access to compare the impact of sample preparation procedures, combinations of liquid chromatography-MS setups, and software differences on critical performance measures across multiple analyses. mpwR supports outputs of commonly used software for bottom-up proteomics like ProteomeDiscoverer, Spectronaut, MaxQuant, and DIA-NN.
Article
Biochemistry & Molecular Biology
Kristina J. H. Kleinwort, Roxane L. Degroote, Sieglinde Hirmer, Lucia Korbonits, Lea Lorenz, Armin M. Scholz, Stefanie M. Hauck, Cornelia A. Deeg
Summary: This study identified a deviant bovine immune phenotype characterized by excessive lymphocyte proliferation after polyclonal stimulation. Proteomic analysis revealed significant differences in the protein profiles between immune deviant and control lymphocytes after immune stimulation.
