Article
Agriculture, Dairy & Animal Science
Po-Yun Teng, Roshan Adhikari, Sara Llamas-Moya, Woo Kyun Kim
Summary: The study found that both mannan-oligosaccharides and beta-glucan improved growth performance in broiler chickens during the starter phase, with beta-glucan additionally increasing body weight gain. Furthermore, the combination of MOS and beta-glucan resulted in higher villi height in the jejunum of the chickens, indicating potential benefits for intestinal health.
Article
Fisheries
Zhuang Wang, Jing Hu, Zhengkang Mei, Yuanxing Zhang, Qin Liu, Dahai Yang
Summary: The study found that mannan-oligosaccharide (MOS) may act as an inducer of trained immunity in teleost fish, protecting turbot from liver injury by modulating metabolic signaling and histone epigenetic modifications.
Article
Agriculture, Dairy & Animal Science
Fernando Gonzalez, Amanda Carelli, Alina Komarcheuski, Mayara Uana, Rodolpho Martin do Prado, Diogo Rossoni, Marcia Gomes, Ricardo Vasconcellos
Summary: This study assessed the effects of adding yeast cell wall compounds (YCWs) to cat food on hindgut fermentation metabolites and fecal microbiota. The results showed that even at low dietary concentrations, YCWs could modulate the metabolism and fecal microbiota traits in cats. This effect was more pronounced in vitro, suggesting that YCWs could potentially contribute to the intestinal health of adult cats fed extruded diets.
Article
Fisheries
Takavar Mohammadian, Reza Ghanei-Motlagh, Taravat Molayemraftar, Mehrzad Mesbah, Mojtaba Zarea, Hamzeh Mohtashamipour, Abdolhossein Jangaran Nejad
Summary: Dietary supplementation with Immunogen? significantly improves growth parameters, carcass protein content, intestinal microflora, and immune responses in shabout fish.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Acoustics
Su Jin Eom, Tae-Gyu Lim, Hyunjhung Jhun, Nam Hyouck Lee, Min-Cheol Kang, Kyung-Mo Song
Summary: The study developed a yeast extract capable of inhibiting melanoma cells using ultrasound technology, showing that it effectively reduces cell growth and proliferation, as well as tumor volume in mice. Optimal extraction conditions resulted in increased total extraction and 8-glucan yields, suggesting that UAEY may represent an effective anti-skin cancer agent.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Yoseph Tsegaye, Phoebe Yeh, Victor Holmes, Matthew Jones, Alexander Kilbo, Chris N. Micklem, Chia-Hong Tsai, Christopher J. Paddon
Summary: Valorization of waste materials is not commonly practiced in industrial fermentation. However, we have developed a system that can convert waste materials into valuable products. By engineering Saccharomyces cerevisiae, we were able to produce both extracellular ss-farnesene and intracellular carotenoids, generating two valuable products in a single fermentation process.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Fisheries
Jicheng Yang, Yaoyao Lin, Zhaohui Wei, Zhenbing Wu, Qianqian Zhang, Jingwen Hao, Shuyi Wang, Aihua Li
Summary: 16S rRNA gene high-throughput sequencing technology was used to investigate the changes in the gut microbiota of yellow catfish infected by Edwardsiella ictaluri. The microbial composition and diversity of the diseased fish were significantly different from those of healthy fish. Proteobacteria became the dominant phylum in the gut of the diseased fish, while Cetobacterium, Plesiomonas, and Romboutsia disappeared. The gut microbiota of the diseased fish showed reduced anaerobic and Gram-positive bacteria, as well as decreased digestive system, immune system, and metabolic functions.
Article
Fisheries
Uni Purwaningsih, Sukenda Sukenda, Angela M. Lusiastuti, Alimuddin Alimuddin, Widanarni Widanarni, Sri Nuryati
Summary: This study compared the phenotypic, genotypic characteristics, and pathogenicity of Edwardsiella ictaluri from different catfish farms in Indonesia. The results showed variations in growth patterns, enzyme activity, antibiotic resistance, and genetic profiles among the isolates. The protein components of E. ictaluri cells were found to be virulent and played a role in the pathogenesis of E. ictaluri infection in catfish.
AQUACULTURE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Ramy S. Yehia, Ahmed M. Saleh, Mohammad Bani Ismail, Saleh Al-Quraishy, Ohoud Al-Amri, Rewaida Abdel-Gaber
Summary: This study investigated the production, characterization, and bioactivity of yeast mannan. The highest production of mannan was observed in the Saccharomyces cerevisiae isolate RY1. Characterization of RY1 mannan revealed its ability to scavenge radicals and exhibit anti-tumor activity against liver and breast cancer cell lines. The findings confirm the potential of S. cerevisiae mannan as a biotherapeutic agent for cancer treatment.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mengqi Fu, Kemin Mao, Jie Gao, Xianghong Wang, Faizan Ahmed Sadiq, Jiale Li, Yaxin Sang
Summary: In this study, the surface layer protein (SLP) from Lactobacillus kefiri HBA20 was characterized and its surface morphology, adhesion potential, and secondary structure were evaluated. The results showed that SLP had a stable structure and the ability to adhere to yeast. Furthermore, molecular docking study revealed the binding mode of SLP with mannan.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemical Research Methods
Surui Lu, Hong Deng, Chenyao Zhou, Zhengda Du, Xuena Guo, Yanfei Cheng, Xiuping He
Summary: Through directed evolution and genetic engineering, the catalytic activity of ss-caryophyllene synthase (CPS) was improved, resulting in increased ss-caryophyllene production in Saccharomyces cerevisiae. The engineering of both the enzyme and the yeast chassis contributed to the significant increase in ss-caryophyllene yield.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Satoshi Uemura, Takashi Moriguchi
Summary: This study elucidates the essential luminal domains of Sur1 and Csh1 dictating substrate specificity and/or protein stabilities via mannan modification.
Article
Biochemistry & Molecular Biology
Jianrong Wu, Congsheng Wang, Tiantian Zhang, Hongtao Zhang, Xiaobei Zhan
Summary: A mannose oligosaccharide-sialic acid conjugate was synthesized to inhibit A β42 aggregation. The mannose oligosaccharides with degree of polymerization of 3-13 were obtained from locust bean gum. The conjugate successfully inhibited A β42 aggregation and showed no cytotoxicity to BV-2 cells. It also reduced the release of pro-inflammatory factor TNF-alpha and inhibited neuroinflammation. This study provides a new approach for the development of glycoconjugates against AD.
CARBOHYDRATE RESEARCH
(2023)
Article
Engineering, Chemical
Guoyu Liu, Yan Yang, Hiroyuki Tachikawa, Xiao-Dong Gao, Hideki Nakanishi
Summary: Beta-glucan, a polysaccharide found in the cell wall of yeast, has immunostimulatory properties. Although spores of yeast also contain beta-glucan, they have been poorly studied as application tools. However, by genetic manipulation, spores can be modified to expose the beta-glucan layer on the cell surface. These modified spores are phagocytosed by mammalian cells in a manner dependent on the receptor dectin-1, and they induce higher levels of proinflammatory cytokines compared to vegetative cells.
Article
Immunology
Jisoo Kim, Soon-Hwan Oh, Rubi Rodriguez-Bobadilla, Vien M. Vuong, Vit Hubka, Xiaomin Zhao, Lois L. Hoyer
Summary: The Pir protein family in Candida albicans is larger than previously recognized and consists of multiple conserved functional motifs that play important roles in the fungal cell wall.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)