Effect of different levels of mannan-oligosaccharide supplementation on some immunological variables in weaned piglets

P>The effect of different doses of mannan-oligosaccharide (MOS) on specific and non-specific immune responses was studied in piglets, weaned at 28 days. A total of 58 piglets were used in six groups. Five groups were fed 0, 1, 2, 4 g MOS product per kg diet or with growth promoting antibiotics and immunized by inactivated Aujeszky's disease virus (AyV) vaccine at week 1 and 3 of the experiment (35 and 49 days). A sixth group, receiving the same non-supplemented diets was not immunized. Blood samples for lymphocyte stimulation (LST) and AyV neutralization (VN) tests were taken from all pigs on the first day of the experiment and at weekly intervals for 5 weeks. At week 8, the immunized piglets were infected orally with transmissible gastroenteritis virus. All piglets were weighed and slaughtered at week 10, digesta from small intestine were collected and tested for the presence of secretory (s)IgA. Feeding MOS supplementation resulted in enhanced specific and non-specific immune responses, however, a regressive dose-response of MOS was observed. Both the specific cellular (LST) and humoral responses (VN) were enhanced after 2 weeks of feeding 1 g/kg MOS and significantly differed from the antibiotic positive control. The same tendency was detected in case of the non-specific LSTs, although these started some weeks later showing significant differences by the fifth week. Higher doses of MOS had no further beneficial effect on systemic immunity. In addition, 1 g/kg MOS supplementation group also showed some advantage in local immune responsiveness. Therefore, based on the studied immune variables, 1 g/kg MOS product is suggested in the diet of weaned piglets.