Journal
JOURNAL OF ANALYTICAL CHEMISTRY
Volume 66, Issue 1, Pages 102-107Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1061934811010217
Keywords
levofloxacin; myoglobin; chemiluminescence; flow-injection; pharmaceuticals; human urine and serum
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Funding
- Shaanxi Province Nature Science Foundation China
- CNPC Tubular Goods Research Institute
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A novel chemiluminescence method for the determination of levofloxacin is presented, which is based on the inhibitory effect of levofloxacin on the chemiluminescence reaction between luminol and myoglobin in a flow-injection system. The decrement of chemiluminescence intensity is linear with the logarithm of levofloxacin concentration over the range from 0.07 to 100.0 ng/mL (r(2) = 0.9994), with the detection limit of 0.02 ng/mL (3 sigma). At a flow rate of 2.0 mL/min, a complete analytical process could be performed within 0.5 min, including sampling and washing, with a relative standard deviation of less than 3.0% (n = 5). The proposed procedure was applied successfully to the determination of levofloxacin in pharmaceutical preparations, human urine and serum without any pretreatment procedure.
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