Journal
NANO LETTERS
Volume 15, Issue 8, Pages 5025-5032Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b01047
Keywords
activatable cell-penetrating peptide; multiplex signals; FRET nanosensor; MT1-MMP; single cell; cancer
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Funding
- NIH [HL098472, HL109142, HL121365, R00CA153914]
- NSF [CBET1360341, DMS1361421]
- UC San Diego, Beckman Laser Institute, Inc.
- NSF Singapore through the Singapore MIT Alliance for Research and Technology's BioSystems and Micromechanics Inter-Disciplinary Research programme
- National Cancer Institute [1R33CA174550]
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We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.
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