Article
Agronomy
Mali Wang, Zhi Tang, Min Liao, Haiqun Cao, Ning Zhao
Summary: A simple and efficient method based on loop-mediated isothermal amplification (LAMP) was developed to detect the Ile-2041-Asn mutation in field populations of Shortawn foxtail (Alopecurus aequalis Sobol.), a grass weed resistant to fenoxaprop-P-ethyl herbicide. The LAMP assay, utilizing a set of four primers targeting a 244-bp fragment of ACCase comprising the codon position 2041, demonstrated high sensitivity and was 1000-fold more sensitive than conventional PCR method. The detection using crude DNA of A. aequalis as a template was also feasible.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Yuning Lan, Wenyu Li, Shouhui Wei, Hongjuan Huang, Zhen Liu, Zhaofeng Huang
Summary: Two black-grass populations in winter wheat fields in China were found to be resistant to clodinafop-propargyl. The resistance was confirmed through dose-response assays and gene sequence analysis, revealing target-site mutations and enhanced metabolism as the resistance mechanisms.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Agronomy
Miriam H. Messelhaeuser, Marcus Saile, Bernd Sievernich, Roland Gerhards
Summary: The study evaluated the control efficacy of cinmethylin and other common pre-emergence herbicides alone and combined with post-emergence herbicides against Alopecurus myosuroides in winter cereals. Late sowing can reduce A. myosuroides density, and herbicides controlled 42-100% of the plants. However, no treatment was consistently better than the others in all experiments.
PLANT SOIL AND ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
Andrea L. Livieri, Facundo Colaccini, Martin A. Hernandez, Gabriela Gago, Hector M. Alvarez, Hugo Gramajo, Eduardo Rodriguez
Summary: In actinomycetes, acyl-CoA carboxylases (ACCs) are biotin-dependent enzymes with diverse structures and functions. Rhodococcus jostii RHA1 was found to have multiple putative ACC domains and subunits. Mutant strains with specific genes disrupted showed the importance of these genes in lipid biosynthesis and growth.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Buli Su, Peixuan Lai, Fan Yang, Anzhang Li, Ming-Rong Deng, Honghui Zhu
Summary: This study developed a novel method to increase the supply of acetyl-CoA in Saccharomyces cerevisiae and improve the production of downstream products. By optimizing metabolic pathways and conducting genetic engineering, the production of mevalonic acid was successfully increased, and further improvement was achieved by dynamically downregulating gene expression. The final strain produced a high yield of lycopene.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agronomy
Zhi Tang, Zilu Wang, Mali Wang, Fan Yin, Min Liao, Haiqun Cao, Ning Zhao
Summary: Shortawn foxtail is a widely distributed wetland plant that has become a noxious weed in winter cropping fields in China. The plant has evolved resistance to the widely used herbicide mesosulfuron-methyl, and researchers identified the resistance mutation and developed a molecular marker to detect it. The study also found that the plant has broad-spectrum cross-resistance to ALS-inhibiting herbicides but remains susceptible to a photosystem II inhibitor.
Article
Geriatrics & Gerontology
Amy Zheng, Seong Eun Kwak, Jesper B. Birk, Edward B. Arias, Dominic Thorley, Jorgen F. P. Wojtaszewski, Gregory D. Cartee
Summary: This study investigated the impact of caloric restriction (CR) on AMP-activated protein kinase (AMPK) and its substrates in skeletal muscle of older animals. The results revealed that CR significantly increased the phosphorylation of AMPK and several AMPK substrates, suggesting a potential role of AMPK in the antiaging effects of CR.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Agronomy
Aristeidis Papapanagiotou, Ioannis Vasilakoglou, Kico Dhima, Ilias Eleftherohorinos
Summary: Late watergrass, a weed in rice fields, has developed resistance to multiple herbicides. However, there is no clear relationship between the growth rate and competitive ability of resistant biotypes.
Article
Microbiology
Pan Wang, Xin Wang, Ying Yin, Mingliang He, Wei Tan, Wenting Gao, Jianping Wen
Summary: The study found that inactivating the signal transduction protein GlnB can increase the production of ascomycin by enhancing the supply of precursors. Protein co-precipitation experiments and coupled enzyme activity assays revealed that the interaction between GlnB protein and the alpha subunit of acetyl-CoA carboxylase and propionyl-CoA carboxylase inhibited their activities.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Tonghui Huang, Xin Wu, Shirong Yan, Tianya Liu, Xiaoxing Yin
Summary: A series of spiroketopyrazole derivatives were synthesized and evaluated for their anticancer activities as ACC inhibitors. Compound 7j showed the strongest enzyme inhibitory activity, while compound 7m exhibited potent anti-proliferative effects against various cancer cell lines, indicating its potential as a therapeutic agent for hepatocellular carcinoma.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Xiaoting Lou, Xiyue Zhou, Haiyan Li, Xiangpeng Lu, Xinzhu Bao, Kaiqiang Yang, Xin Liao, Hanxiao Chen, Hezhi Fang, Yanling Yang, Jianxin Lyu, Hong Zheng
Summary: The study suggests that ACC1 deficiency leads to patient symptoms such as global developmental delay, microcephaly, hypotonia, and dysmorphic facial features. Cellular experiments revealed disruptions in lipid metabolism and cell motility capacity caused by ACC1 deficiency, which could be partially alleviated by palmitate.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Microbiology
John E. Cronan
Summary: This review provides an update on the research of Escherichia coli acetyl-CoA carboxylase (ACC), focusing on the structures, stoichiometry, regulation of ACC activity and expression.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Ryo Mizojiri, Daisuke Tomita, Masako Sasaki, Yoshihiko Satoh, Yukiko Yamamoto, Hiroyuki Sumi, Hironobu Maezaki
Summary: By modifying the molecular length, a new ACC1-selective inhibitor 1i was discovered. Further chemical modification produced compound 1n with potent ACC1 selective inhibition and suitable PK profile for in vivo evaluations. This novel series of potent ACC1-selective inhibitors shows promise as both research tools and potential therapeutic agents.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Alissa M. Williams, Olivia G. Carter, Evan S. Forsythe, Hannah K. Mendoza, Daniel B. Sloan
Summary: While the chloroplast is mainly known for its function in photosynthesis, it also plays a crucial role in other metabolic processes in plants. This study focuses on the ongoing gene duplications and rate variations in two key chloroplast enzymes, acetyl-CoA carboxylase and caseinolytic protease, involved in fatty acid biosynthesis and protein turnover, respectively. The results suggest that gene duplication and rate variation continue to shape the plastid proteome.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2022)
Article
Oncology
Fei-Yuan Yu, Qian Xu, Qi-Yao Wei, Hai-Ying Mo, Qiu-Hua Zhong, Xiao-Yun Zhao, Andy T. Y. Lau, Yan-Ming Xu
Summary: ACC2 is under-expressed in cancerous tissue and negatively correlated with tumor size, lymph-node metastases, and clinical stage in lung adenocarcinoma. Knocking down ACC2 promotes cell proliferation and migration, and affects the expression of cell cycle-related genes. Lung adenocarcinoma patients with under-expressed ACC2 have a worse prognosis.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ning Zhao, Yanyan Yan, Weitang Liu, Jinxin Wang
Summary: Multiple herbicide resistance in diverse weed species is a threat to herbicide sustainability and global food safety. In the case of shortawn foxtail, CYP709C56 enzyme plays a key role in metabolic resistance by degrading mesosulfuron-methyl.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Minghao Jiang, Yafei Wang, Wei Li, Qi Li, Jingxu Zhang, Min Liao, Ning Zhao, Haiqun Cao
Summary: Chinese sprangletop populations in Anhui Province, China have developed widespread resistance to the herbicide cyhalofop-butyl, with target site mutations being one of the main resistance mechanisms.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Agronomy
Cuixia Wu, Min Song, Tiantian Zhang, Chao Zhou, Weitang Liu, Tao Jin, Ning Zhao
Summary: This study reveals that the L. multiflorum population HN-03 has evolved high-level resistance to the herbicide clodinafop-propargyl due to a target-site mutation. The population also exhibits cross-resistance and multiple-resistance to other herbicides, but remains susceptible to cypyrafluone. Pre-treatment with piperonyl butoxide and malathion enhances the susceptibility of the HN-03 population to certain herbicides. Cypyrafluone can be used as an alternative herbicide for resistant L. multiflorum.
Article
Agriculture, Multidisciplinary
Dawei Zhang, Xiangju Li, Feng Bei, Tao Jin, Sisi Jia, Ruotong Bu, Jinxin Wang, Hengzhi Wang, Weitang Liu
Summary: This study identified a herbicide-resistant population of Aegilops tauschii, and explored the non-target site resistance mechanism through transcriptome sequencing and reference genome analysis. The results showed that cytochrome P450 monooxygenase (P450) and glutathione S-transferase (GST) play crucial roles in the enhanced metabolism of mesosulfuron-methyl.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agronomy
Mali Wang, Zhi Tang, Min Liao, Haiqun Cao, Ning Zhao
Summary: A simple and efficient method based on loop-mediated isothermal amplification (LAMP) was developed to detect the Ile-2041-Asn mutation in field populations of Shortawn foxtail (Alopecurus aequalis Sobol.), a grass weed resistant to fenoxaprop-P-ethyl herbicide. The LAMP assay, utilizing a set of four primers targeting a 244-bp fragment of ACCase comprising the codon position 2041, demonstrated high sensitivity and was 1000-fold more sensitive than conventional PCR method. The detection using crude DNA of A. aequalis as a template was also feasible.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Cheng Yang, Ruihong Sun, Xingtao Lu, Tao Jin, Xuegang Peng, Na Zhang, Jinxin Wang, Hengzhi Wang, Weitang Liu
Summary: This study investigated the seed germination and seedling emergence of Vicia villosa under various conditions. The results showed that V. villosa seeds had high germination rates under different temperatures, photoperiods, salt stress levels, pH, osmotic stress levels, and burial depths. V. villosa, as a cover crop, has considerable potential to be planted and grown in orchards in China and can contribute to early weed management and the establishment of V. villosa populations.
Article
Agronomy
Xiangyang Qin, Cheng Yang, Mengmeng Hu, Yunxia Duan, Na Zhang, Jinxin Wang, Hengzhi Wang, Weitang Liu
Summary: Black-grass population HN-14 in Chinese wheat fields has developed resistance to mesosulfuron-methyl through target-site mutation. It also exhibits varying degrees of resistance to fenoxaprop-P-ethyl, clodinafop-propargyl, haloxyfop-methyl, and fluazifop-P-butyl, while remaining sensitive to other herbicides.
Article
Agronomy
Wei Li, Cuixia Wu, Mali Wang, Minghao Jiang, Jingxu Zhang, Min Liao, Haiqun Cao, Ning Zhao
Summary: The distribution and resistance status of Italian ryegrass in China's Huang-Huai-Hai Plain is crucial for the integrated management of this weed in winter wheat production systems.
Article
Agriculture, Multidisciplinary
Lei Lian, Hengzhi Wang, Fengwen Zhang, Weitang Liu, Xingtao Lu, Tao Jin, Jinxin Wang, Xiuhai Gan, Baoan Song
Summary: This study confirmed the inhibitory effect of cypyrafluone on 4-hydroxyphenylpyruvate dioxygenase (HPPD). Cypyrafluone caused severe foliar whitening symptoms and plant death within a short period of time. It was found that cypyrafluone decreases the content of chlorophyll pigments and inhibits the catalytic activity of 4-HPPD. Molecular docking analysis showed that cypyrafluone binds well to the active site of HPPD and forms a bidentate coordination interaction with the Fe2+ atom.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agronomy
Qi Li, Ning Zhao, Minghao Jiang, Mali Wang, Jingxu Zhang, Haiqun Cao, Min Liao
Summary: This study reports the occurrence of metabolic metamifop resistance in the worldwide E. glabrescens. The high-level metamifop resistance in the LJ-02 population may be mainly due to specific isoforms of GSTs that possess high catalytic activity and strong substrate specificity.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Zhi Tang, Zilu Wang, Mali Wang, Fan Yin, Min Liao, Haiqun Cao, Ning Zhao
Summary: Shortawn foxtail is a widely distributed wetland plant that has become a noxious weed in winter cropping fields in China. The plant has evolved resistance to the widely used herbicide mesosulfuron-methyl, and researchers identified the resistance mutation and developed a molecular marker to detect it. The study also found that the plant has broad-spectrum cross-resistance to ALS-inhibiting herbicides but remains susceptible to a photosystem II inhibitor.
Article
Agronomy
Shanshan Yin, Wei Hu, Yin Chai, Minghao Jiang, Jingxu Zhang, Haiqun Cao, Ning Zhao, Min Liao
Summary: The invasive weed smallflower umbrella sedge has developed resistance to the widely used herbicide bensulfuron-methyl in Chinese rice fields. The resistant population of the weed showed cross-resistance to other herbicides but remained susceptible to certain alternative options. This study provides insights into managing herbicide resistance in aggressive weeds.
Article
Biology
Cheng Yang, Hao Wang, Yunxia Duan, Feng Bei, Sisi Jia, Jinxin Wang, Hengzhi Wang, Weitang Liu
Summary: In this study, the resistance mechanisms of HW-01 and ST-1 populations of Amaranthus retroflexus to fomesafen and nicosulfuron were investigated. The study confirmed the target-site resistance mechanism and the non-target-site resistance mechanism based on cytochrome P450s and GSTs enzymes in the HW-01 population. This research provides scientific and theoretical guidance for controlling resistant populations of A. retroflexus.
Article
Biology
Xumiao Wang, Wei Hu, Yuxi Li, Minghao Jiang, Ning Zhao, Haiqun Cao, Min Liao
Summary: This study reports a suspected nicosulfuron-resistant population of large crabgrass in Chinese maize fields. The population showed a high level of resistance to nicosulfuron, which was primarily attributed to enhanced metabolism involving P450 enzymes.
