4.7 Article

Intestinal Anti-inflammatory Effects of Oligosaccharides Derived from Lactulose in the Trinitrobenzenesulfonic Acid Model of Rat Colitis

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 19, Pages 4285-4297

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/jf500678p

Keywords

intestinal anti-inflammatory effect; lactulose-derived oligosaccharides; prebiotic; TNBS rat colitis

Funding

  1. Spanish Ministry of Economy and Competitivity [SAF2011-29648]
  2. Consolider-Ingenio [FUNC-C-FOOD CSD 2007-00063]
  3. Junta de Andalucia [AGR-6826, CTS 164]
  4. European Union
  5. Instituto de Salud Carlos III

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Intestinal microbiota modulation is becoming an interesting approach to manage inflammatory bowel disease and can be achieved by the administration of prebiotics. Previous studies showed the intestinal anti-inflammatory effects of the prebiotic lactulose. The aim of the present study was to test the preventative effects of oligosaccharides derived from lactulose with prebiotic properties (OsLu) in the trinitrobenzenesulfonic acid model of rat colitis and compare them with those of lactulose. Both treatments modified bacterial profile in intestinal contents, increasing the bifidobacteria and lactobacilli counts and up-regulating the production of short-chain fatty acids, although OsLu generated a larger amount. OsLu also inhibited to a greater extent different pro-inflammatory markers such as interleukins (IL) 1, 6, 12, and 23 and chemokines (MCP-1 and CINC-1). However, both prebiotics equally restored colonic epithelial integrity, evaluated both with a histological score (OsLu, 9.8 +/- 2.2; and lactulose, 12.1 +/- 2.1, vs colitic control, 27.3 +/- 3.3) and by measuring several key proteins of the mucosal barrier (MUC-2, MUC-3, and TTF-3). OsLu effect was also associated with an inhibition of iNOS expression and a reduction of Th17 cell activity in the inflamed tissue that facilitated the intestinal mucosa barrier recovery. In conclusion, OsLu showed a better anti-inflammatory profile than lactulose in this model of experimental colitis.

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