Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 44, Pages 10687-10693Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf503755m
Keywords
ion chromatography; biopesticide; glucosinolates; mustard analysis; oilseed
Funding
- Agriculture and Food Research Initiative competitive grant from the USDA National Institute of Food and Agriculture [2011-67009-20094]
- NIFA [579806, 2011-67009-20094] Funding Source: Federal RePORTER
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Although mustards such as Sinapis alba and Brassica juncea contain glucosinolates (sinalbin and sinigrin, respectively) that hydrolyze to form biopesticidal products, routine quality control methods to measure active ingredients in seed and seed meals are lacking. We present a simple and fast ion chromatography method for the simultaneous quantification of sinigrin, sinalbin, and anionic hydrolysis products in mustard seed to assess biological potency. Optimum conditions include isocratic elution with 100 mM NaOH at a flow rate of 0.9 mL/min on a 4 X 210 mm hydroxide-selective anion-exchange column. All anion analytes including sinigrin, sinalbin, SO42-, and SCN- yielded recoveries ranging from 83 to 102% and limits of detection =0.04 mM, with samples displaying little interference from plant matrix components. Sample preparation is minimized and analysis times are shortened to <90 min as compared with previous methods that took days and multiple instruments.
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