Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 2, Pages 419-426Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf404786v
Keywords
anti-pyridoxine; linatine; 1-amino-D-proline; flaxseed
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- NSERC CREATE
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In the present study, the anti-pyridoxine compounds linatine (1-[(n-gamma-L-glutamyl)aminc]-D-proline) and 1-amino-D-proline (1ADP) were quantified following extraction from defatted flaxseed using aqueous isopropanol as a solvent, with extraction variables including time, temperature, and the solid/solvent ratio. Both linatine and 1ADP were identified, characterized, and quantified via UPLC/ESI-MS using authentic standards. To optimize the extraction conditions for these anti-pyridoxine compounds, a response surface methodology was applied using a, second-order polynomial to describe the experimental data. The predicted model for the optimal extraction was significant (P < 0.05) with a R-2 of 0.82. A varietal analysis showed that the amount of anti-pyridoxine present in flaxseed ranged from 177 to 437 mu g 1ADPE/g of whole seed. The current study establishes the content of specific anti-pyridoxine factors in flaxseed and positions the data for use in subsequent risk assessment modeling.
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