Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 38, Pages 9279-9285Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf503143g
Keywords
MALDI-MS imaging; strictinin; green tea; ellagitannin; distribution
Funding
- MEXT Funding-Project for Developing Innovation Systems-Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program in Japan
- JSPS KAKENHI [22228002, 26660115]
- Grants-in-Aid for Scientific Research [26660115] Funding Source: KAKEN
Ask authors/readers for more resources
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualizing the distribution of a wide range of biomolecules within tissue sections. However, methodology for visualizing a bioactive ellagitannin has not yet been established. This paper presents a novel in situ label-free MALDI-MSI technique for visualizing the distribution of strictinin, a bioactive ellagitannin found in green tea, within mammalian kidney after oral dosing. Among nine representative matrix candidates, 1,5-diaminonaphthalene (1,5-DAN), harmane, and ferulic acid showed higher sensitivity to strictinin spotted onto a MALDI sample plate. Of these, 1,5-DAN enables visualization of a two-dimensional image of strictinin directly spotted on mouse kidney sections with the highest sensitivity. Furthermore, 1,5-DAN-based MALDI-MSI could detect the unique distribution of orally dosed strictinin within kidney sections. This in situ label-free imaging technique will contribute to the localization analysis of strictinin and its biological mechanisms.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available