4.7 Article

Validation of Caffeine Dehydrogenase from Pseudomonas sp Strain CBB1 as a Suitable Enzyme for a Rapid Caffeine Detection and Potential Diagnostic Test

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 62, Issue 31, Pages 7939-7946

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/jf501598c

Keywords

caffeine; caffeine dehydrogenase; Pseudomonas sp CBB1; diagnostic test; nursing mother's milk

Funding

  1. University of Iowa Research Funds

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Excess consumption of caffeine (>400 mg/day/adult) can lead to adverse health effects. Recent introduction of caffeinated products (gums, jelly beans, energy drinks) might lead to excessive consumption, especially among children and nursing mothers, hence attracting the Food and Drug Administration's attention and product withdrawals. An in-home test will aid vigilant consumers in detecting caffeine in beverages and milk easily and quickly, thereby restricting its consumption. Known diagnostic methods lack speed and sensitivity. We report a caffeine dehydrogenase (Cdh)-based test which is highly sensitive (1-5 ppm) and detects caffeine in beverages and mother's milk in 1 min. Other components in these complex test samples do not interfere with the detection. Caffeine-dependent reduction of the dye iodonitrotetrazolium chloride results in shades of pink proportional to the levels in test samples. This test also estimates caffeine levels in pharmaceuticals, comparable to high-performance liquid chromatography. The Cdh-based test is the first with the desired attributes of a rapid and robust caffeine diagnostic kit.

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