Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 61, Issue 37, Pages 8724-8729Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf4021619
Keywords
brewer's spent yeast; flavor enhancers; HILIC; mechanic yeast disruption; nucleotides; nucleosides; serial repitching
Funding
- [PEst-C/EQB/LA0006/2011]
- Fundação para a Ciência e a Tecnologia [PEst-C/EQB/LA0006/2011] Funding Source: FCT
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The present work evaluates the influence of serial yeast repitching on nucleotide composition of brewer's spent yeast extracts produced without addition of exogenous enzymes. Two procedures for disrupting cell walls were compared, and the conditions for low-cost and efficient RNA hydrolysis were selected. A HILIC methodology was validated for the quantification of nucleotides and nucleosides in yeast extracts. Thirty-seven samples of brewer's spent yeast (Saccharomyces pastorianus) organized according to the number of serial repitchings were analyzed. Nucleotides accounted for 71.1-88.2% of the RNA products; 2'AMP was the most abundant (ranging between 0.08 and 2.89 g/100 g dry yeast). 5'GMP content ranged between 0.082 and 0.907 g/100 g dry yeast. The sum of 5'GMP, 5'IMP, and 5'AMP represented between 25 and 32% of total nucleotides. This works highlights for the first time that although serial repitching influences the content of monophosphate nucleotides and nucleosides, the profiles of these RNA hydrolysis products are not affected.
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