Article
Biotechnology & Applied Microbiology
Feronika Heppy Sriherfyna, Minenosuke Matsutani, Kensuke Hirano, Hisashi Koike, Naoya Kataoka, Tetsuo Yamashita, Eiko Nakamaru-Ogiso, Kazunobu Matsushita, Toshiharu Yakushi
Summary: Gluconobacter oxydans oxidizes glucose in the periplasmic space independently of nicotinamide cofactors, but if this oxidation system is eliminated, glucose is oxidized in the cytoplasm. Two types of NADH dehydrogenase in G. oxydans have different substrate specificities, with the auxiliary NDH playing a crucial role in maintaining cell growth in the absence of the periplasmic oxidation system.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Alexa M. Schmitz, Brooke Pian, Sean Medin, Matthew C. Reid, Mingming Wu, Esteban Gazel, Buz Barstow
Summary: Researchers developed a whole-genome knockout collection of mutants for Gluconobacter oxydans, identifying genes influencing the efficacy of rare earth element bioleaching. Disruption of genes related to Pyrroloquinoline Quinone synthesis and glucose dehydrogenase significantly reduces bioleaching, while disruption of phosphate-specific transport system genes enhances bioleaching efficiency. This study offers a roadmap for engineering G. oxydans' genome to improve bioleaching efficiency.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Svenja Battling, Tobias Engel, Elena Herweg, Paul-Joachim Niehoff, Matthias Pesch, Theresa Scholand, Marie Schoepping, Nina Sonntag, Jochen Buechs
Summary: The study optimized the production process of 5-keto-d-fructose, successfully increasing both the titre and productivity through different feeding strategies, and demonstrated the scale-up of the process to a 150 L pressurized fermenter.
MICROBIAL CELL FACTORIES
(2022)
Article
Agricultural Engineering
Jian Han, Xia Hua, Xin Zhou, Bin Xu, Huan Wang, Guohong Huang, Yong Xu
Summary: The study demonstrates that diatomite detoxification can effectively achieve commercial production of bio-based chemicals like xylonic acid, reducing costs by recycling bacterial cells. Additionally, a scale-up production of xylonic acid was achieved in a sealed oxygen supply fermenter. Overall, diatomite treatment provides an efficient and practical approach for the production of biochemicals.
BIORESOURCE TECHNOLOGY
(2021)
Article
Food Science & Technology
Edicon T. S. Chan, Yan Zhu, Xiu-Zhen Li, Ting Zhou, Stephen Y. K. Seah
Summary: Patulin is a mycotoxin that contaminates apples and apple products, but can be transformed into E-ascladiol by extracts from Gluconobacter oxydans ATCC 621. The identification and characterization of enzymes involved in the transformation provide insights into their potential application for patulin detoxification.
Article
Biotechnology & Applied Microbiology
Hongsen Zhang, Jiahui Jiang, Conghui Quan, Guizhong Zhao, Guotao Mao, Hui Xie, Fengqin Wang, Zhimin Wang, Jian Zhang, Pingping Zhou, Andong Song
Summary: The aldehyde dehydrogenase gene W826-RS0111485 in Gluconobacter oxydans DSM2003 plays a crucial role in the conversion of aldehyde inhibitors. The enzyme showed the highest oxidation activity to furfural among several aldehyde inhibitors, and NADP was determined to be the most suitable cofactor. The recombinant E. coli containing the W826-RS0111485 gene successfully converted furfural to furoic acid within 8 hours, demonstrating its importance in furfural detoxification.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Li Liu, Yue Chen, Shiqin Yu, Jian Chen, Jingwen Zhou
Summary: In this study, key D-sorbitol dehydrogenases in Gluconobacter oxydans WSH-003 were identified through gene knockout. By knocking out dehydrogenase genes, the production of L-sorbose was significantly enhanced. G. oxydans-30 showed reduced by-product formation, while G. oxydans MD-16 achieved the highest conversion ratio. The systematic engineering of dehydrogenases greatly improved L-sorbose production.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xinlei Mao, Baoqi Zhang, Chenxiu Zhao, Jinping Lin, Dongzhi Wei
Summary: The productivity of d-xylonic acid can be improved in Gluconobacter oxydans by overexpressing the mGDH gene, leading to increased tolerance to hydrolysate inhibitors. The recombinant strain G. oxydans/pBBR-R3510-mGDH shows high productivity and tolerance, making it a promising choice for biotechnological production.
MICROBIAL CELL FACTORIES
(2022)
Article
Chemistry, Physical
Leonardo Tensi, Luca Rocchigiani, Gabriel Menendez Rodriguez, Edoardo Mosconi, Cristiano Zuccaccia, Filippo De Angelis, Alceo Macchioni
Summary: The reaction mechanism of nicotine amide dinucleotide hydrogenation (NAD(+) to NADH) catalyzed by Cp*Ir(pyridine-2-sulfonamidate) complexes in the presence of phosphonic acid has been elucidated. The enhanced performance of these catalysts stems from the hemilability of the pyridine ligand, which is displaced during P-H bond activation and facilitates the generation of the metal-hydride intermediate. Experimental results are supported by DFT calculations showing the importance of hydrogen bonding interactions in the activation of phosphite anions. Direct comparison between the unsubstituted catalyst and the 6-aminopyridine-2-sulfonamidate derivative reveals the molecular origin of the latter's superior performance, paving the way for the intelligent design of better catalysts for NADH regeneration.
CATALYSIS SCIENCE & TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yue Chen, Li Liu, Shiqin Yu, Jianghua Li, Jingwen Zhou, Jian Chen
Summary: The acetic acid bacterium Gluconobacter oxydans is widely used in industrial production due to its unique incomplete oxidation process, with 2-keto-L-gulonic acid (2-KLG) being one of the compounds produced. Promoters play a crucial role in controlling gene expression, and in this study, the low activity of SDH in G. oxydans was addressed by enhancing SDH expression using newly identified promoters. The study identified several strong promoters that significantly increased the yield of 2-KLG in G. oxydans, providing new tools for metabolic engineering in the production of high-value products.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Weizhu Zeng, Xiaoyu Shan, Li Liu, Jingwen Zhou
Summary: In this study, a high-yield DHA synthesis strain was selected among five industrial wild-type Gluconobacter strains. By individually knocking out unrelated dehydrogenase genes, DHA production was significantly enhanced. Further optimization of culture media and the use of a fed-batch fermentation system resulted in high production of DHA.
BIORESOURCES AND BIOPROCESSING
(2022)
Article
Biochemistry & Molecular Biology
Yue Chen, Dong Li, Xiaoyu Shan, Jingwen Zhou, Jian Chen
Summary: In this study, the sorbose dehydrogenase of Gluconobacter oxydans WSH-004 was identified as a sorbose oxidase (SOX). The study also found that H2O2 inhibited the activity of SOX and identified phenazine methosulfate (PMS) as the best electron acceptor for SOX. Furthermore, the enzymatic catalysis of SOX and SNDH was conducted in vitro to produce 2-KLG from L-sorbose.
PROCESS BIOCHEMISTRY
(2022)
Article
Metallurgy & Metallurgical Engineering
Abhilash, Sabrina Hedrich, Axel Schippers
Summary: The study demonstrated that bioleaching of scandium from red mud using Gluconobacter oxydans microorganism can be highly effective. Under specific conditions, the solubilization of Sc in Indian and German red mud reached 83% and 94%, respectively. Additionally, the total amount of gluconic acid excreted by the bacteria increased with an increase in pulp density.
Article
Food Science & Technology
Nisa Saelee, Ling-Zhi Cheong, Manat Chaijan
Summary: This research investigates the enhancement of acetic acid production in the mangosteen vinegar fermentation process through mixed-culture fermentation and analyzes the qualities and properties of the resulting mangosteen vinegar. The findings highlight the significant influence of the unpasteurized condition on acetic acid production and suggest potential health benefits of the mangosteen vinegar due to its elevated levels of total phenolic content, total flavonoid content, and anti-DPPH radical activity.
Article
Electrochemistry
Taiki Adachi, Yuki Kitazumi, Osamu Shirai, Kenji Kano
Summary: The bioelectrocatalytic properties of membrane-bound aldehyde dehydrogenase (AlDH) from Gluconobacter oxydans NBRC12528 were evaluated, showing direct electron transfer (DET)-type activity for acetaldehyde oxidation at various electrodes. The coordination of CN with AlDH at the multi-walled carbon nanotube-modified electrode changed the direction of DET-type bioelectrocatalysis under acidic conditions. These findings were discussed in terms of thermodynamics.
ELECTROCHEMISTRY COMMUNICATIONS
(2021)
Article
Chemistry, Applied
Lujia Zhang, Li Dong, Liuqing Yang, Yinghua Luo, Fang Chen
Summary: Acrylamide, a potential carcinogen formed in high-heat carbohydrate-rich foods, induces mitochondrial dysfunction. The overexpression of miR-27a-5p regulates mitochondrial dysfunction, with Btf3 identified as its target gene. The miR-27a-5p-Btf3-ATM-p53 axis plays a crucial role in promoting AA-induced cell apoptosis by disrupting mitochondrial structure and function.
Article
Biochemistry & Molecular Biology
Lin Yao, Xinyi Wang, Rui Xue, Hong Xu, Rui Wang, Lujia Zhang, Sha Li
Summary: The study successfully expressed mussel foot protein fp-3B in Escherichia coli for the first time, and found that co-expression with tyrosinase from Verrucomicrobium spinosum significantly increased production yield and modification level of fp-3B, enhancing its adhesive ability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Materials Science, Ceramics
Lujia Zhang, Guannan Li, Xin Gong, Jianfeng Tang, Yuming Lu
Summary: In this study, Tm-doped 0.7Bi(1-x)Tm(x)FeO(3-0.3)BaTiO(3) ceramics were fabricated and their structural, morphological, dielectric, and multiferroic properties were investigated. The addition of Tm led to a phase transition and significant improvement in the dielectric and multiferroic properties. Increasing Tm content showed a similar trend for remnant polarization and remnant magnetization, indicating a possible strong magnetoelectric coupling effect.
CERAMICS INTERNATIONAL
(2022)
Article
Clinical Neurology
Xiaoqin Yang, Hyun Kyoo Yoo, Suvina Amin, Wendy Y. Cheng, Sanjana Sundaresan, Lujia Zhang, Mei Sheng Duh
Summary: This study aimed to evaluate the clinical and humanistic burden of pediatric patients with NF1-PN in the USA. The findings showed that these patients experienced symptoms such as pain, disfigurement, and motor dysfunction. Their overall quality of life was significantly affected, including physical, emotional, social, and school functioning. Debunking surgeries, although used for symptom management, were associated with complications and hospitalizations.
CHILDS NERVOUS SYSTEM
(2022)
Article
Chemistry, Applied
Yulu Miao, Hui Ni, Xingyi Zhang, Fengdong Zhi, Xiang Long, Xuepeng Yang, Xiao He, Lujia Zhang
Summary: Understanding the interactions between sweet taste receptors and various sweeteners through molecular docking and constructing sweetener-T1R2-membrane systems reveals differences in how natural and artificial sweeteners bind to the receptor, with significant van der Waals interactions playing a crucial role in stabilizing high-intensity sweeteners. This study provides insights into the mechanisms of sweetener function and offers new directions for sweetener design.
Article
Biochemistry & Molecular Biology
Yue Pan, Jinxiao Bao, Xingyi Zhang, Hui Ni, Yue Zhao, Fengdong Zhi, Bohuan Fang, Xiao He, John Z. H. Zhang, Lujia Zhang
Summary: A new strategy was reported to improve the AM selectivity in the production of aromatic aldehydes, combining rational design based on the butterfly model and experimental validation methods. Through analysis of key residuals and generation of mutants, a high AM selectivity above 99% was successfully achieved.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Engineering, Biomedical
Chuanxi Zhang, Yulu Miao, Yinghui Feng, Jiawei Wang, Zhuoli Tian, Juan Dong, Bei Gao, Lujia Zhang
Summary: This study establishes a system to detect umami peptides targeting human T1R1 receptors and investigates the molecular mechanisms involved. The results show that different peptide binding sites and peptide hydrophobicity play important roles in determining taste thresholds and affinity. This research enables rapid screening of high-intensity umami peptides and the development of T1R1 receptor-based umami detection sensors.
Article
Biochemistry & Molecular Biology
Lujia Zhang, Liuqing Yang, Yinghua Luo, Li Dong, Fang Chen
Summary: This review summarizes the recent advances in the mechanism of hepatotoxicity through AA-induced oxidative stress in rodent livers and hepatic cell lines, which is beneficial for assessing the risks of AA exposure and exploring effective intervention methods for AA hepatotoxicity.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biotechnology & Applied Microbiology
Zidong Jiang, Lujia Zhang, Wei Zhou, Hui Li, Yuanyi Li, Weichuang Qin, Fengqing Wang, Dongzhi Wei, Bei Gao
Summary: In this study, a binding structure between SecA and a signal peptide was established, and a grinding approach was used to modify them, leading to improved protein secretion efficiency. A bidirectional grinding strategy was also developed to enhance the secretion system, resulting in a significantly increased protein activity. This research provides a novel and promising strategy for optimizing protein secretion and production in B. subtilis.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Mingzhe Ma, Yalong Cong, John Z. H. Zhang, Lujia Zhang
Summary: Enzymatic hydrolysis of food-derived proteins can produce bioactive peptides that activate food functions, such as antihypertension. However, the diversity of hydrolysis products can reduce the efficacy of bioactive peptides. Highly specific proteases can homogenize the hydrolysis products, reducing the production of impotent peptides.
Article
Biochemistry & Molecular Biology
Xinyi Wang, Xinxin Feng, Rui Xue, Hong Xu, Rui Wang, Lujia Zhang, Sha Li
Summary: In this study, the soluble expression of mussel foot proteins (Mfps) was increased by using a small ubiquitin-related modifier (SUMO) and thioredoxin A (TrxA) as catalysts. Co-expression of Mfp-3 with SUMO-TrxA resulted in an 18.07% increase in soluble expression. The combination also boosted the soluble expression of hybrid Mfps. High cell density cultivation further increased the yields of Mfps, indicating the potential for sustainable production of protein adhesive materials.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Jie Heng, Yunfei Hu, Guillermo Perez-Hernandez, Asuka Inoue, Jiawei Zhao, Xiuyan Ma, Xiaoou Sun, Kouki Kawakami, Tatsuya Ikuta, Jienv Ding, Yujie Yang, Lujia Zhang, Sijia Peng, Xiaogang Niu, Hongwei Li, Ramon Guixa-Gonzalez, Changwen Jin, Peter W. Hildebrand, Chunlai Chen, Brian K. Kobilka
Summary: Research reveals the dynamic behavior of the beta 2 adrenergic receptor C-terminus and its autoinhibitory function in downstream Gs coupling.
NATURE COMMUNICATIONS
(2023)
Article
Food Science & Technology
Mingzhe Ma, Yinghui Feng, Yulu Miao, Qiang Shen, Shuting Tang, Juan Dong, John Z. H. Zhang, Lujia Zhang
Summary: Chronic diseases, such as hypertension, have detrimental effects on human health. Conventional drugs for treatment often come with significant side effects. Food-sourced ACE inhibitory peptides provide a safer alternative, with fewer side effects. However, the lack of a systematic and effective screening method and understanding of the sequence characteristics and molecular mechanisms hinder the development of ACE inhibitory peptides.
Article
Food Science & Technology
Liuqing Yang, Li Dong, Lujia Zhang, Daotong Li, Yinghua Luo, Fang Chen
Summary: This study investigated the role of disrupted autophagy-lysosomal pathway (ALP) in acrylamide (AA)-induced neurotoxicity. AA was found to induce autophagy activation and ALP dysfunction, which contributed to apoptosis and NLRP3 inflammasome pathway activation. The study also showed that decreased expression of transcription factor EB (TFEB) was responsible for AA-induced ALP dysfunction, and TFEB overexpression restored lysosomal-associated proteins and protected against AA-induced neurotoxicity. These findings provide novel insights into the mechanisms of AA-induced neurotoxicity and suggest TFEB as a potential therapeutic target.
Article
Chemistry, Applied
Jianbiao Gao, Yihua Qiu, Fu Chen, Lujia Zhang, Wei Wei, Xingye An, Qiaomei Zhu
Summary: Two kinds of nanocellulose (CNFs and CNCs) were synthesized from pomelo peels through TEMPO oxidation and sulfuric acid treatment, respectively. The FTIR results confirmed the complete removal of hemicellulose and lignin from the pomelo peel cellulose substrate. The obtained CNFs and CNCs exhibited uniform morphology and nanoscale particle size. CNF-based Pickering emulsions showed higher stability compared to emulsions stabilized with CNCs due to the formation of a gel structure induced by the longer fibrils of CNFs. Increased oil fractions enhanced the viscoelasticity of CNF-based Pickering emulsions. In vitro digestion results indicated that higher oil fractions reduced the lipolysis degree due to the larger droplet size and higher viscoelasticity of the emulsion. The release of lycopene during gastrointestinal digestion followed a similar trend to that of free fatty acid release, suggesting that higher oil fractions were beneficial for controlling lycopene release.