Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 61, Issue 40, Pages 9734-9743Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf4012054
Keywords
T-2 toxin; trichothecenes; metabolites; rat; UPLC-Q/TOF-MS
Funding
- National Basic Research Program of China [2009CB118801]
- International Science and Technology Cooperation Program of China [2012DFG31840]
- National Institute of Environmental Health Sciences [P42ES013661]
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In the present study, metabolites of T-2 toxin in in vivo and in vitro systems of Wistar rats were identified and elucidated by ultraperformance liquid chromatography-quadrupole/time-of-flight tandem mass spectrometry (UPLC-Q/T0EMS). Expected and unexpected metabolites were detected by Metabolynxxs software, which could automatically compare MSE data from the sample and control. A total of 19 metabolites of T-2 toxin were identified in this research, 9 of them being novel, which were 15-deacetyl-T-2, 3'-OH-15-deacetyl-T-2, 3',7-dihydroxy-T-2, isomer of 3',7-dihydroxy-T-2, 7-0H-HT-2, isomer of 7OH-HT-2, de-epoxy-3',7-dihydroxy-HT-2, 9-0H-T-2, and 3',9-dihydroxy-T-2. The results showed that the main metabolic pathways of T-2 toxin were hydrolysis, hydroxylation, and de-epoxidation. In addition, the results also revealed one novel metabolic pathway of T-2 toxin, hydroxylation at C-9 position, which was demonstrated by the metabolites 9-0H-T-2 and 3',9dihydroxy-T-2. In addition, hydroxylation at C-9 of T-2 toxin was also generated in in vitro of liver systems. Interestingly, several metabolites of hydroxylation at C-7 of T-2 toxin were also detected in in vivo male Wistar rats, but they were not found in in vivo female rats and in in vitro systems of Wistar rats.
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