4.7 Article

Germination Dramatically Increases Isoflavonoid Content and Diversity in Chickpea (Cicer arietinum L.) Seeds

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 60, Issue 35, Pages 8606-8615

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/jf3021514

Keywords

chickpea; germination; isoflavones; pterocarpan phytoalexins; antioxidant capacity

Funding

  1. National University of Singapore Virtual Institute for the Study of Aging (VISA) [R-143-000-437-290]

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The effect of germination on bioactive components in legume seeds was investigated in terms of the antioxidant capacity and total phenolic contents. Gemination increased the total phenolic content and antioxidant capacity of most seeds. Particularly in chickpea seeds, the isoflavone contents increased by over 100 fold, mainly due to the increase of formononetin and biochanin A level. As a result, these two compounds were conveniently isolated from the germinated seeds in preparative scale and structurally confirmed by UV-vis, ESI-MS, and H-1 NMR spectroscopies. Isoflavonoid fingerprints analyzed by HPLC-PDA and LC-ESI-MS demonstrated that germination could significantly increase isoflavonoids diversity. Twenty-five isoflavonoids were detected and identified tentatively. These include 20 isoflavones, 2 isoflavanones, and 3 pterocarpan phytoalexins. Total isoflavonoid content of germinated chickpea was approximately 5-fold of that of germinated soybean. Our findings suggest that the germinated chickpea seeds could serve as a promising functional food rich in isoflavonoids.

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