Journal
NANO LETTERS
Volume 15, Issue 10, Pages 6357-6364Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b01356
Keywords
conformational changes; protein nanofiber; AAO template; FRET; extrusion
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Funding
- Max Planck Society
- European Research Council under the European Union [294852]
- BMBF/MPG network MaxSynBio
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Fibronectin is present in the extracellular matrix and can be assembled into nanofibers in vivo by undergoing conformational changes. Here, we present a novel approach to prepare fibronectin nanofibers under physiological conditions using an extrusion approach through nanoporous aluminum oxide membranes. This one-step process can prepare nanofiber bundles up to a millimeter in length and with uniform fiber diameters in the nanometer range. Most importantly, by using different pore diameters and protein concentrations in the extrusion process, we could induce varying lasting structural changes in the fibers, which were monitored by Forster resonance energy transfer and should impose different physiological functions.
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