Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 59, Issue 11, Pages 5914-5918Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf200459s
Keywords
Genetically modified maize; loop-mediated isothermal amplification; event-specific
Funding
- National Transgenic Plant Special Fund [2008ZX08012-002, 2008ZX08012-005, 2009ZX08012-002B, 2011ZX08012-002]
- National Key Basic Research Program [2007CB109201]
- Shanghai Rising-Star Program [11QA1403300]
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As more and more genetically modified (GM) crops are approved for commercialization and planting, the development of quick and on-spot methods for GM crops and their derivates is required. Herein, we established the polymerase chain reaction and agarose gel electrophoresis-free system for the identification of seven GM maize events (DAS-59122-7, T25, BT176, TC1507, MON810, BT11, and MON863) employing a loop-mediated isothermal amplification (LAMP) technique. The LAMP assay was performed using a set of four specific primers at 60-65 degrees C in less than 40 min, and the results were observed by direct visual observation. In these developed assays, the specificity targeted at each GM maize event based on the event-specific sequence was well confirmed, and the limits of detection were as low as four copies of maize haploid genomic DNA with an exception of 40 copies for MON810 assay. Furthermore, these developed assays were successfully used to test six practical samples with different GM maize events and contents (ranged from 0.0 to 2.0%). All of the results indicated that the established event-specific visual LAMP assays are more convenient, rapid, and low-cost for GM maize routine analysis.
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