Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 59, Issue 15, Pages 8047-8057Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf201501x
Keywords
broccoli; glucosinolates; LC-MS/MS; glucobrassicin metabolites; glucoraphanin metabolites; sulforaphane; indole-3-carbinol; selenium
Funding
- Federal Ministry of Education and Research (BMBF) [0313846D]
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This study aimed at developing a sensitive LC-MS/MS method for the quantification of sulforaphane (SFN) and indole-3-carbinol metabolites in plasma and urine after dietary intake of regular and selenium-fertilized broccoli using stable isotope dilution analysis. In a three-armed, placebo-controlled, randomized human intervention study with 76 healthy volunteers, 200 g of regular (485 mu g of total glucosinolates and <0.01 mu g of selenium per gram fresh weight) or selenium-fertilized broccoli (589 mu g of total glucosinolates and 0.25 mu g of selenium per gram fresh weight) was administered daily for 4 weeks. Glucoraphanin and glucobrassicin metabolites quantified in plasma and urine were SFN-glutathione, SFN-cysteine, SFN-cysteinylglycine, SFN-acetylcysteine, and indole-3-carboxaldehyde, indole-3-carboxylic acid, and ascorbigen, respectively. Dietary intake of selenium-fertilized broccoli increased serum selenium concentration analyzed by means of atomic absorption spectroscopy by up to 25% (p < 0.001), but affected neither glucosinolate concentrations in broccoli nor their metabolite concentrations in plasma and urine compared to regular broccoli.
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