Article
Biochemistry & Molecular Biology
Junwei Wang, Shuxiang Mao, Mantian Liang, Wenxia Zhang, Fangzhen Chen, Ke Huang, Qiuyun Wu
Summary: This study found that exogenous methyl jasmonate (MeJA) treatment can promote glucosinolate (GSL) biosynthesis and sulforaphane accumulation in broccoli, with a more pronounced effect on florets. Additionally, MeJA treatment increases flavonoid content and antioxidant activity in broccoli florets.
Article
Chemistry, Applied
Junwei Wang, Shuxiang Mao, Qi Wu, Yiming Yuan, Mantian Liang, Shengze Wang, Ke Huang, Qiuyun Wu
Summary: Red light promotes GSL biosynthesis and sulforaphane accumulation, while blue light inhibits them. LED light sources regulate the biosynthesis of GSLs and sulforaphane in broccoli through the modulation of gene expression and metabolic pathways.
Article
Agronomy
Qingxi Yang, Qian Zhou, Xin Zhou, Huixin Fang, Yingbo Zhao, Baodong Wei, Shujuan Ji
Summary: Postharvest yellowing is the main manifestation of rapid deterioration in broccoli, leading to a significant reduction in glucosinolates content. The reduction in glucoraphanin and sulforaphane content is mainly attributed to the continuous down-regulation of related genes.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yongqi Yin, Yin Liu, Chao Cheng, Zhengfei Yang, Zhenlan Luo, Weiming Fang
Summary: Exogenous melatonin enhances the tolerance of broccoli sprouts to ZnSO4 stress, promotes glucoraphanin biosynthesis, and increases the content of isothiocyanates. The molecular mechanism underlying the role of melatonin in isothiocyanate metabolism under ZnSO4 stress was investigated using physio-biochemical methods, quantification of gene expression levels, and iTRAQ labelling technique.
Article
Nutrition & Dietetics
John A. Bouranis, Laura M. Beaver, Jaewoo Choi, Carmen P. Wong, Duo Jiang, Thomas J. Sharpton, Jan F. Stevens, Emily Ho
Summary: Research indicates that the production of isothiocyanates may be influenced by the composition of the gut microbiome, potentially impacting the efficacy of interventions with cruciferous vegetables. In humans, there is significant inter-individual variability in SFN-NIT excretion following consumption of broccoli sprouts.
Article
Horticulture
Junwei Wang, Shuxiang Mao, Yiming Yuan, Na Zhang, Qi Wu, Mantian Liang, Shengze Wang, Ke Huang, Qiuyun Wu
Summary: The study investigated the effects of storage conditions and cooking methods on the content of chlorophyll, GSL, and sulforaphane in broccoli florets. Results showed that chlorophyll, GSLs, and sulforaphane content decreased with prolonged storage time, with the best preservation performance observed with 1-MCP treatment and 0 degrees C storage condition. Cooking methods also influenced the content of these compounds, with steaming for 7 min or microwaving for 5 min found to be the most effective in preserving the quality and increasing sulforaphane content in broccoli florets.
Article
Chemistry, Applied
Xinxing Xu, Shuang Bi, Fei Lao, Fang Chen, Xiaojun Liao, Jihong Wu
Summary: The study investigated the metabolism of bioactive compounds in broccoli juice fermented by animal- and plant-derived Pediococcus pentosaceus. Results showed that P. pentosaceus may potentially improve the nutritional and functional properties of fermented foods.
Article
Agronomy
Betiana S. Trod, Melina S. Buttarelli, Maria M. Stoffel, Gabriel Ceccoli, Laura Olivella, Pamela B. Barengo, Merce Llugany, Maria G. Guevara, Fernando F. Munoz, Lucas D. Daurelio
Summary: Broccoli heads are valuable vegetable crops due to their high nutritional content, but they quickly decompose after harvest. Previous research has shown that foliar application of sodium selenate and selenite can delay this process. This study analyzed the effect of these preharvest treatments on the postharvest shelf-life of broccoli and found that selenium treatments not only delayed yellowing but also maintained the firmness, diameter, and nutritional composition of the heads.
Article
Agronomy
Shi-hui Liu, Qiu-yun Wu, Ting Xu, Hui-cong Wang, Jun-wei Wang, Zhong-Lin Zhang, Ming-yue Liu, Ke Huang
Summary: This study investigated the medicinal potential of Chinese broccoli and found significant variations in the content of sulforaphane, an active anticancer ingredient, among different germplasms. Cluster analysis based on sulforaphane content and qualitative phenotypic traits classified the germplasms into five different groups. These findings not only confirmed the medicinal values of Chinese broccoli but also provided crucial information for future germplasm development and new variety breeding.
Article
Agronomy
Yuting Li, Xuetao Han, Hao Ren, Bin Zhao, Jiwang Zhang, Baizhao Ren, Huiyuan Gao, Peng Liu
Summary: With global warming, high-temperature stress has become a major abiotic stress for crops, in particular summer maize in China. The effects of exogenous SA or 6-BA on growth, photosynthesis capacity, photosystem II activity, subcellular ultrastructure, antioxidant system, and plant hormones in maize leaves under HT stress were investigated.
Article
Food Science & Technology
Yi-Yuan Ke, Yuan-Tay Shyu, Sz-Jie Wu
Summary: High-pressure processing can increase the isothiocyanate content in broccoli, while improving total phenolic content and enhancing nutritional value. This method also stimulates myrosinase activity, prevents loss of heat-sensitive components, and effectively inhibits oxidases to maintain food quality. Additionally, high pressures have anti-inflammatory and antioxidant effects, making it a potential treatment strategy for healthy foods.
Article
Food Science & Technology
John A. Bouranis, Carmen P. Wong, Laura M. Beaver, Sandra L. Uesugi, Ethan M. Papenhausen, Jaewoo Choi, Edward W. Davis, Adilson Nunes Da Silva, Newton Kalengamaliro, Rekha Chaudhary, Jordan Kharofa, Vinita Takiar, Thomas J. Herzog, William Barrett, Emily Ho
Summary: This study examined the bioavailability of sulforaphane (SFN) and the microbiome profile in subjects fed a single serving of fresh broccoli microgreens. The results showed that broccoli microgreens are a significant source of SFN and do not significantly alter microbiome composition.
Article
Food Science & Technology
Andrea Mahn, Carmen Elena Perez, Victor Zambrano, Herna Barrientos
Summary: Blanching conditions were investigated to maximize sulforaphane content in broccoli sprouts. Higher temperatures and shorter immersion times were found to increase sulforaphane content. The optimum conditions were blanching at 61 degrees C for 4.8 min, resulting in a 3.3-fold increase in sulforaphane content compared to untreated sprouts. This process could contribute to the development of functional foods and nutraceuticals containing sulforaphane.
Article
Agronomy
Yiwei Zhou, Jinmei Liu, Yechun Xu, Jianjun Tan, Genfa Zhu, Yuanjun Ye
Summary: This study found that soaking and spraying 6-BA can significantly extend the vase life of Curcuma alismatifolia cut flowers, particularly the variety 'Siam Shadow'. Physiological, biochemical, and transcriptomic analyses revealed the involvement of antioxidant enzymes, jasmonic acid, and senescence-associated transcription factors in delaying bract senescence. The findings suggest that 6-BA can act as a potential preservative by affecting gene expression related to JA biosynthesis and signal transduction, antioxidant enzymes, and senescence-associated transcription factors.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sajad Shokri, Hema Jegasothy, Mary Ann Augustin, Netsanet Shiferaw Terefe
Summary: The study showed that thermosonication can enhance the yield of sulforaphane in broccoli, despite a decrease in myrosinase activity. The highest yield was achieved under the treatment of thermosonication at 60 degrees Celsius for 7 minutes, which also increased the residual level of sulforaphane, indicating the potential for converting broccoli biomass into high sulforaphane ingredients.
Article
Food Science & Technology
Chunbo Song, Kang Wang, Xiang Xiao, Qingli Liu, Minjie Yang, Xu Li, Yabin Feng, Saisai Li, Liyu Shi, Wei Chen, Zhenfeng Yang
Summary: This study revealed the mechanism of chilling injury (CI) and protective mechanisms in peach fruit during storage at different low temperatures. It found that 4°C storage promoted the occurrence of CI and stimulated protective mechanisms, while 0°C delayed the occurrence of CI. These findings provide important insights into the understanding of CI and cold tolerance in peach fruit.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Agronomy
Xiang Xiao, Minjie Yang, Wanqi Dong, Chujiang Zhou, Liyu Shi, Wei Chen, Shifeng Cao, Zhenfeng Yang, Saisai Li
Summary: The study found that gibberellic acid treatment can delay senescence and chlorophyll degradation in postharvest okras, and can negatively regulate genes and transcription factors involved in chlorophyll degradation.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Horticulture
Xiang Xiao, Liyu Shi, Wanqi Dong, Shuwan Jin, Qingli Liu, Wei Chen, Shifeng Cao, Zhenfeng Yang
Summary: The study revealed that ethylene treatment increased carotenoid content in peach fruit, promoting fruit coloration and softening, while 1-MCP treatment had the opposite effect. Gene expression analysis showed that ethylene and 1-MCP treatments affected the expression of carotenoid synthetic genes and transcription factors in postharvest peaches.
SCIENTIA HORTICULTURAE
(2022)
Review
Plant Sciences
Juan Xiang, Shen Rao, Qiangwen Chen, Weiwei Zhang, Shuiyuan Cheng, Xin Cong, Yue Zhang, Xiaoyan Yang, Feng Xu
Summary: Selenium is an essential trace element for humans and animals, and tea leaves contain beneficial organic selenium. Exogenous selenium treatment can significantly improve the growth of tea trees and the nutritional quality of tea leaves, providing theoretical and technical support for breeding selenium-rich tea.
Review
Plant Sciences
Wei Chao, Shen Rao, Qiangwen Chen, Weiwei Zhang, Yongling Liao, Jiabao Ye, Shuiyuan Cheng, Xiaoyan Yang, Feng Xu
Summary: Selenium is an essential trace element for human immune regulation and disease prevention. In plants, organic selenium compounds promote plant growth, improve nutritional quality, and play important roles in plant ecosystems. Selenium accumulation plants release selenium-containing compounds that promote the growth of selenium accumulators but inhibit the growth and distribution of non-selenium accumulators.
Article
Chemistry, Applied
Wanqi Dong, Liyu Shi, Saisai Li, Feng Xu, Zhenfeng Yang, Shifeng Cao
Summary: The effect of hydrogen-rich water (HRW) treatment on softening, cell wall components, and cell wall metabolic genes in postharvest okras was studied. The results showed that HRW treatment could delay softening, maintain fruit firmness, and extend shelf life. This was achieved by altering cell wall components, up-regulating biosynthesis genes, and down-regulating degradative genes at different stages of storage.
Article
Agronomy
Xu Li, Zeyang Bao, Yana Chen, Qingqing Lan, Chunbo Song, Liyu Shi, Wei Chen, Shifeng Cao, Zhenfeng Yang, Qingbo Zheng
Summary: This study found that exogenous GSH treatment can inhibit weight loss and alleviate browning in postharvest okra, maintaining the appearance and quality of the fruit. GSH treatment also reduces the levels of ROS and regulates redox homeostasis in okra. Therefore, exogenous GSH treatment can prolong the shelf life of okra.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Forestry
Ke Yang, Zhongbing Liu, Xueyin Chen, Xian Zhou, Jiabao Ye, Feng Xu, Weiwei Zhang, Yongling Liao, Xiaoyan Yang, Qijian Wang
Summary: As the most significant transformation stage of plants, the flowering process has been a focus of research. This study identified 26 MADS-box genes from Ginkgo biloba and analyzed their gene structures, protein features, and expression patterns. The findings suggest that these genes may play an important role in the development and differentiation of G. biloba flowers.
Article
Plant Sciences
Chujiang Zhou, Wanqi Dong, Shuwan Jin, Qingli Liu, Liyu Shi, Shifeng Cao, Saisai Li, Wei Chen, Zhenfeng Yang
Summary: The treatment of peach fruit with GABA can reduce chilling injury during cold storage by up-regulating the biosynthetic genes and candidate transcription factors related to ascorbic acid and glutathione.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Applied
Qingli Liu, Xu Li, Shuwang Jin, Wanqi Dong, Yong Zhang, Wei Chen, Liyu Shi, Shifeng Cao, Zhenfeng Yang
Summary: The effect of GABA treatment in postharvest kiwifruit was studied, and it was found that GABA treatment resulted in higher chilling tolerance and better quality maintenance. This was attributed to the increase in AsA levels, which helped protect cell membranes and alleviate chilling-induced oxidative stress. GABA treatment also upregulated the expression of AsA metabolic genes and candidate transcription factors involved in AsA biosynthesis.
Article
Biology
Saisai Li, Yijuan Zhang, Liyu Shi, Shifeng Cao, Wei Chen, Zhenfeng Yang
Summary: In this study, we isolated and identified the R2R3-MYB type transcription factor, MrMYB9, through comparative transcriptome analysis of red and white Chinese bayberry fruits. The expression of MrMYB9 was positively correlated with anthocyanin levels and biosynthetic gene expression during fruit maturation. Our findings shed light on the biosynthesis of anthocyanins during color maturation in Chinese bayberry.
Article
Horticulture
Li Yu, Qiangwen Chen, Xiaoli Liao, Xiaoyan Yang, Wei Chao, Xin Cong, Weiwei Zhang, Yongling Liao, Jiabao Ye, Hua Qian, Yang Zhao, Shuiyuan Cheng, Feng Xu
Summary: Selenium is an important trace element for human and animal health. Approximately 0.5-1 billion people worldwide suffer from selenium deficiency, leading to various endemic diseases. Cabbage, as a popular vegetable, can accumulate selenium through biofortification. Therefore, producing selenium-enriched cabbage may be an effective solution to alleviate selenium deficiency.
Article
Engineering, Chemical
Yabin Feng, Kui Suo, Liqiang Chen, Hafida Wahia, Cunshan Zhou, Zhenfeng Yang, Xianhui Chang, Liyu Shi, Wei Chen, Chunbo Song, Saisai Li
Summary: A synergistic pretreatment of artificial perforation and alcohol maceration (PAT) was proposed to improve the drying of garlic. The results showed that PAT pretreatment significantly reduced drying time and energy consumption, and improved the quality of dried garlic.
Article
Food Science & Technology
Chunbo Song, Zhenfeng Yang, Xiang Xiao
Summary: This study investigated the role of dynamic changes in membrane lipid metabolism in GABA-mediated cold tolerance of peach fruits. GABA treatment reduced phosphatidic acid accumulation, attenuated its biosynthesis, and maintained higher levels of phosphatidylcholine and phosphatidylethanolamine, leading to improved cold tolerance. GABA treatment protected peaches against low-temperature stress by maintaining higher levels of specific lipids and enhancing the accumulation of diacylglycerol and triacylglycerol. This study provides novel insights into the mechanisms underlying cold tolerance in postharvest peach fruits.
Article
Plant Sciences
Chunbo Song, Mengbo Wu, Ying Zhou, Zehao Gong, Weiwei Yu, Yi Zhang, Zhenfeng Yang
Summary: The research identified two cold-responsive NAC transcription factors in bananas that negatively regulate cold tolerance in banana fruits by upregulating the expression of phospholipid degradation genes, forming a positive feedback loop to induce phospholipid degradation and produce phosphatidic acid. Ethylene was found to directly inhibit phospholipid degradation in banana and transgenic tomato fruits.
HORTICULTURE RESEARCH
(2022)