4.7 Article

Extraction of Proteins with Low Fluoride Level from Antarctic Krill (Euphausia superba) and Their Composition Analysis

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 59, Issue 11, Pages 6108-6112

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/jf201009t

Keywords

Euphausia superba; krill proteins; fluoride; protein solubilization; protein precipitation; fluoride removal

Funding

  1. International Science and Technology Cooperation Project [2010DFA31330]
  2. Ministry of Science and Technology of China

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The extraction of proteins with low fluoride level (LFP) from Antarctic krill (Euphausia superba) was investigated in this work The optimal conditions for protein solubilization were determined to be pH 11.5 and 4 degrees C. The proteins were solubilized two times; a water/krill ratio (mL/g) of 6 and a time of 30 min were used for the first step, whereas the second used a water/krill residue ratio (mL/g) of 3 and a time of 30 min. The optimum pH for protein precipitation was 4.6. A LFP with fluoride content of 9.86 mg/kg (dry weight) was finally obtained through a fluoride removal program. The protein yield of LFP was 52.68%. Composition analysis of LFP indicated it was composed of 66.96% of crude proteins (dry weight) and 33.01% of total lipids (dry weight)(n) and all nine essential amino acids were in sufficient amounts to meet FAO/WHO/UNU requirements for adults and infants. In addition, LFP could be taken as a good source of EPA and DHA for consideration of use as a food item for human consumption.

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