4.7 Article

In Vitro Evaluation on the Antiobesity Effect of Lignans from the Flower Buds of Magnolia denudata

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 59, Issue 10, Pages 5665-5670

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/jf200230s

Keywords

Magnolia denudata; antiobesity effect; lignan; (+)-epimagnolin; (+)-magnolin

Ask authors/readers for more resources

In the present study, an attempt has been made to isolate antiobesity components from crude extracts of the flower buds of Magnolia denudata by CH2Cl2 and MeOH solvents. The crude extracts were partitioned into n-hexane, 85% aqueous MeOH, n-butanol, and water fractions. Their antiobesity effects were evaluated by measuring the effect on adipogenic differentiation using 3T3-L1 cells. Among the fractions, n-hexane and 85% aqueous MeOH fractions effectively reduced the lipid accumulation and the regulation of the adipogenic transcription factor. Both n-hexane and 85% aqueous MeOH fractions were further separated by diverse chromatographic methods to give four lignans (A-D). In comparative analysis, the presence of the lignans during adipogenic differentiation reduced the absorbance values of eluted Oil Red 0 solution in the order of potency C > D > B > A. Moreover, C and D effectively downregulated SREBP1, PPAR gamma, and C/EBP alpha.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available