Article
Agriculture, Multidisciplinary
M. Luisa Hernandez, Cristina Munoz-Ocana, Pilar Posada, M. Dolores Sicardo, Damaso Hornero-Mendez, Raquel B. Gomez-Coca, Angjelina Belaj, Wenceslao Moreda, Jose M. Martinez-Rivas
Summary: The study investigates the variability of squalene content in a collection of 36 olive cultivars and identifies two cultivars with extremely high and low squalene content. The researchers also clone cDNA sequences encoding squalene synthases from olive and determine the gene mainly involved in squalene biosynthesis. The study highlights the transcriptional regulation of squalene biosynthesis in water-stressed olive mesocarp.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sergio Cerezo, M. Luisa Hernandez, Elena Palomo-Rios, Naima Gouffi, Lourdes Garcia-Vico, M. Dolores Sicardo, Carlos Sanz, Jose A. Mercado, Fernando Pliego-Alfaro, Jose M. Martinez-Rivas
Summary: The investigation on the functional analysis of the olive 13-HPL gene through overexpression and silencing demonstrated its important role in the biosynthesis of olive volatile compounds, impacting the quality of virgin olive oil. The study revealed that altering the expression levels of 13-HPL gene resulted in significant changes in the volatile profile of the leaves, affecting plant growth and development.
Article
Plant Sciences
M. Luisa Hernandez, Samuele Moretti, M. Dolores Sicardo, Ursula Garcia, Ana Perez, Luca Sebastiani, Jose M. Martinez-Rivas
Summary: Three different cDNA sequences encoding phospholipid:diacylglycerol acyltransferases (PDAT) have been isolated from olive, showing distinct features of the PDAT family. The expression of these PDAT genes is spatially and temporally regulated in different olive tissues, potentially contributing to triacylglycerol biosynthesis. Regulation of PDAT genes by environmental factors in the olive fruit mesocarp suggests their involvement in response to abiotic stresses.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Chaim Engelen, Tahel Wechsler, Ortal Bakhshian, Ilan Smoly, Idan Flaks, Tamar Friedlander, Giora Ben-Ari, Alon Samach
Summary: With global warming, it is crucial to understand how warmer winters will affect olive flower induction for predicting future olive oil production sustainability. This study investigated the impact of fruit load, winter drought, and different temperature regimes on olive flower induction. The findings highlighted the importance of studying trees without previous fruit load and showed that soil water content during winter does not significantly affect flower induction. Additionally, the study proposed an efficient method to calculate accumulated chill units correlated with flower induction.
Article
Horticulture
Jose Miguel Garcia-Garvi, Luis Noguera-Artiaga, Francisca Hernandez, Antonio Jose Perez-Lopez, Armando Burgos-Hernandez, Angel A. Carbonell-Barrachina
Summary: Olive oil, a basic product in the Mediterranean diet, is increasingly popular worldwide due to its nutritional value. However, the drought in Mediterranean countries necessitates water control mechanisms to restrict water supply without compromising product quality. This study analyzed the impact of deficit irrigation on olive oil, showing that it leads to oil that is more favored by consumers, with higher concentrations of aromatic compounds, polyunsaturated fatty acids, and antioxidant capacity.
Article
Horticulture
Jinhua Li, Xinyue Ji, Zhaoshan Wang, Yanfei Zeng, Jianguo Zhang
Summary: This study aimed to investigate the morphological and molecular characteristics of two inter-subspecific F-1 hybrids obtained by crossing olive cultivars with pollen donors from olive subspecies. The study identified significant differences in morphological traits between genotypes and confirmed the inter-specific hybrid nature of the two hybrids through molecular analysis.
Article
Plant Sciences
Meagan van Dyk, Christoffel F. J. Spies, Lizel Mostert, Marieta van der Rijst, Ihan L. du Plessis, Providence Moyo, Wynand J. van Jaarsveld, Francois Halleen
Summary: A recent survey in South Africa identified several fungi associated with olive trunk disease symptoms, some of which had not been reported before. Pathogenicity tests were conducted to determine the virulence of these species, with some showing significant lesions on olive branches. Pseudophaeomoniella globosa was identified as one of the main olive trunk pathogens due to its high incidence and virulence in trials.
Article
Plant Sciences
Meagan Van Dyk, Christoffel F. J. Spies, Lizel Mostert, Francois Halleen
Summary: This study reveals that pruning debris serves as an inoculum source of P. globosa in established olive orchards in South Africa. It emphasizes the importance of reducing the inoculum sources of this pathogen as an additional management strategy against olive trunk diseases.
Review
Plant Sciences
Rogerio Melloni, Elke J. B. N. Cardoso
Summary: International research has focused on studying the effects of different management practices in olive cultivation on soil. Both conventional techniques, such as using herbicides and machines to control weeds, inorganic fertilizers, and pesticides, and sustainable techniques, including pruning, the use of disease and climate-tolerant cultivars, organic conditioners, maintaining vegetation cover and using inoculants, have been examined. The impacts on soil quality, crop development, and production are evaluated by studying the presence, activity, and behavior of microorganisms in the soil or directly in the crop.
Article
Biochemistry & Molecular Biology
George T. Tziros, Anastasios Samaras, George S. Karaoglanidis
Summary: The study investigated the potential use of PRIs and a BCA for managing olive leaf spot, with control efficacy over 68% and higher efficacy with dual applications. Laminarin showed the highest control efficacy of 100% and induced different kinetics of gene expression, which could contribute to a better understanding of PRIs in olive culture and the reduction of copper accumulation in the environment.
Review
Agronomy
Giora Ben-Ari, Iris Biton, Yair Many, Dvory Namdar, Alon Samach
Summary: Climate change impacts olive reproduction and oil quality, requiring adaptation through breeding programs. It is crucial to define pathways controlling fruit productivity and oil quality to cope with current and predicted climate changes. Urgent designation of heat-resistant olive cultivars is necessary to address these crucial changes.
Article
Biochemistry & Molecular Biology
Soraya Mousavi, Roberto Mariotti, Maria Cristina Valeri, Luca Regni, Emanuele Lilli, Emidio Albertini, Primo Proietti, Daniela Businelli, Luciana Baldoni
Summary: This study evaluated the salt tolerance of four olive cultivars and identified key genes involved in salt stress response. The results showed that Koroneiki was the most salt-tolerant cultivar, while Fadak86 and Picual were the most susceptible ones. The expression analysis of candidate genes suggested the important roles of OeNHX7, OeP5CS, OeRD19A, and OePetD in activating a salt tolerance mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sandra Martins, Ermelinda Silva, Catia Brito, Carlos Martins-Gomes, Alexandre Goncalves, Margarida Arrobas, Manuel Angelo Rodrigues, Carlos M. Correia, Fernando M. Nunes
Summary: Soil degradation and climate change pose threats to rainfed olive orchards in the Mediterranean, but the use of soil amendments can improve soil fertility and enhance crop yield and olive oil quality.
Editorial Material
Plant Sciences
Carolina Sanchez-Romero
Summary: Somatic embryogenesis in olive is a pivotal in vitro technique with various biotechnological applications, but faces challenges in practical implementation. This editorial paper focuses on summarizing the contributions and discussing future research prospects in this field.
Article
Agronomy
Anja Novoselic, Dora Klisovic, Igor Lukic, Marina Lukic, Karolina Brkic Bubola
Summary: The study found that adding an appropriate amount of olive leaf during olive oil extraction can increase oil yield, extractability, pigments concentration, and positive sensory attributes. However, excessive addition of leaf may have negative effects on the phenolic composition of the oil.
Article
Chemistry, Applied
Rosa Maria Alonso-Salces, Luis Angel Berrueta, Beatriz Quintanilla-Casas, Stefania Vichi, Alba Tres, Maria Isabel Collado, Carlos Asensio-Regalado, Gabriela Elena Viacava, Aimara Ayelen Poliero, Enrico Valli, Alessandra Bendini, Tullia Gallina Toschi, Jose Manuel Martinez-Rivas, Wenceslao Moreda, Blanca Gallo
Summary: This study utilized H-1 NMR fingerprinting of edible oils and multivariate classification and regression models to accurately identify olive oils and their blends with other vegetable oils, providing support for regulations and control bodies. The models showed stable binary classification for olive oil type and precise determination of percentage of vegetable oil in the mixture. The approach demonstrated satisfactory performance with blind samples, confirming its potential for authenticity and traceability assurance.
Article
Engineering, Environmental
Angeles Trujillo-Reyes, Erika Sinisgalli, Juan Cubero-Cardoso, Ana G. G. Perez, Antonio Serrano, Rafael Borja, Fernando G. G. Fermoso
Summary: This study investigates the direct effect of different mechanical treatments on the chemical composition and anaerobic digestion of lignocellulosic substrates. The results show that mechanical treatments can increase the solubilisation of substrates, resulting in enhanced anaerobic digestion.
Article
Biochemistry & Molecular Biology
Andres Notario, Rosario Sanchez, Pilar Luaces, Carlos Sanz, Ana G. Perez
Summary: This study analyzed the changes in the biosynthesis of volatile and phenolic compounds in olive fruits induced by B. oleae infestation. Infested fruits showed increased content of certain volatile compounds and decreased phenolic content. The study also discovered the presence of a PPO-mediated oxidative defense system in olives.
Article
Food Science & Technology
Alberto Navarro, Maria-Victoria Ruiz-Mendez, Carlos Sanz, Melchor Martinez, Duarte Rego, Ana G. Perez
Summary: The use of pulsed electric fields technology in olive oil extraction can significantly increase oil yield and extraction efficiency, as well as enhance the phenolic and volatile compound content in olive oil. PEF treatment does not affect the physicochemical parameters and tocopherol content of olive oil, and sensory evaluation shows that PEF-extracted oils have better taste.
Article
Biochemistry & Molecular Biology
Ana M. Pastrana, Celia Borrero, Ana G. Perez, Manuel Aviles
Summary: Fusarium oxysporum f. sp. fragariae and Macrophomina phaseolina are soilborne fungi that cause significant economic losses to strawberry growers worldwide. The symptoms caused by both pathogens are similar and include vascular discoloration, wilting, stunting, and dieback of plants. However, the infection of these pathogens can alter the content of certain phenolic and volatile compounds in the strawberry plants, which have antifungal and antioxidant properties. These changes in aroma profiles not only have sensory and economic implications but also play a role in the plant's defense system against pathogens. This line of research has potential for developing new tools for detecting and controlling soil pathogens.
Article
Biochemistry & Molecular Biology
Rosario Sanchez, Laura Arroyo, Pilar Luaces, Carlos Sanz, Ana G. Perez
Summary: Phenolic compounds containing hydroxytyrosol have a significant impact on the functional properties and health benefits of virgin olive oil. This study identified and characterized olive polyphenol oxidase (PPO) genes to evaluate their role in the metabolism of hydroxytyrosol-derived compounds. Four PPO genes were synthesized, cloned, and expressed in Escherichia coli, and their functional identity was confirmed using olive phenolic substrates. Among these genes, OePPO2 showed strong diphenolase activity in the oxidative degradation of phenols during oil extraction and appeared to play a vital role in the natural defense mechanism against biotic stress, while OePPO3 encoded a tyrosinase protein with both diphenolase and monophenolase activity, catalyzing the hydroxylation of tyrosol to form hydroxytyrosol.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Antonia M. Gallardo-Martinez, Julia Jimenez-Lopez, Maria Luisa Hernandez, Juan Manuel Perez-Ruiz, Francisco Javier Cejudo
Summary: The absence of 2-Cys Prxs leads to inhibition of seed development in Arabidopsis, highlighting the significance of plastid redox systems in embryogenesis.
Article
Agriculture, Multidisciplinary
M. Luisa Hernandez, Cristina Munoz-Ocana, Pilar Posada, M. Dolores Sicardo, Damaso Hornero-Mendez, Raquel B. Gomez-Coca, Angjelina Belaj, Wenceslao Moreda, Jose M. Martinez-Rivas
Summary: The study investigates the variability of squalene content in a collection of 36 olive cultivars and identifies two cultivars with extremely high and low squalene content. The researchers also clone cDNA sequences encoding squalene synthases from olive and determine the gene mainly involved in squalene biosynthesis. The study highlights the transcriptional regulation of squalene biosynthesis in water-stressed olive mesocarp.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Horticulture
Hande Yilmaz-Duzyaman, Maria G. Medina-Alonso, Carlos Sanz, Ana G. Perez, Raul de la Rosa, Lorenzo Leon
Summary: This study assessed the influence of genotype and environment on the phenolic content and composition in extra virgin olive oil. The results showed that genotype had a stronger effect than environment, and there were significant variations among different cultivars. These findings suggest the feasibility of breeding new cultivars with distinctive phenolic content and composition.
Article
Horticulture
Maria Gomez-del-Campo, Ana G. Perez, Jose M. Garcia
Summary: This study analyzed the quality parameters of oils from seven different varieties of olives grown in hedgerow under cold conditions. The oils were evaluated for hydrolytic, oxidative, and sensory deterioration, as well as their content in photosynthetic pigments, fatty acids, and phenolic compounds. The study found a strong correlation between oxidative stability and the ratio of MUFA/PUFA and the content of secoiridoid phenolic compounds. Genetic factors were found to have a major influence on the phenolic composition of the oils. All of the oils remained in the extra category, although there were significant differences in UV absorbance and phenolic content among the varieties.