Article
Agriculture, Dairy & Animal Science
Yi Wang, Wei Luo, Beibei Wang, Di Wu, Jinqiu Wang, Fang Geng
Summary: A qualitative and relative quantitative analysis of metabolites during egg yolk formation was conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results revealed that amino acids, organic acids, and their derivatives were the main metabolite categories involved in providing material support for embryonic development. Additionally, vitamins and carbohydrates were consumed during follicular development to support normal development. The small hierarchical follicle (SF) period was found to play a critical role in the regulation of transport and deposition of certain active ingredients.
Article
Environmental Sciences
Yueyue Li, Yashu Li, Guanxi Wang, Julin Li, Meihua Zhang, Jingyao Wu, Cheng Liang, Huayun Zhou, Jianxia Tang, Guoding Zhu
Summary: Insecticide resistance is a severe global problem for vector control. A recent study analysed the molecular metabolite changes in deltamethrin-resistant An. sinensis mosquitoes using liquid chromatography tandem mass spectrometry (LC-MS/MS) and found that the differential metabolites were mainly associated with glycerophospholipid metabolism. These findings provide further mechanistic understanding of insecticide resistance in An. sinensis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Xian Luo, Liang Li
Summary: Cellular metabolomics provides insights into metabolic processes in cells, and this study investigates the effects of solvent evaporation equipment and storage conditions on high-coverage cellular metabolomics analysis. The choice of solvent evaporation equipment does not significantly impact the cellular metabolome, but the presence of methanol during storage can lead to notable changes in the metabolome after 7 days. Thoroughly drying cell samples before storage or shipment at room temperature is recommended to ensure high-quality results.
Article
Biochemical Research Methods
Pauline Bosman, Valerie Pichon, Ana Carolina Acevedo, Helene Chardin, Audrey Combes
Summary: Advancements in metabolomics have allowed for the identification and characterization of saliva metabolites as biomarkers, but discrepancies in biomarker content for a given disease have been observed. Differences in saliva collection, storage, and treatment methods could contribute to these discrepancies, as there is currently no standardized method. This study investigated the effects of different collection methods on saliva metabolite analysis, and found that saliva obtained through spitting and aspiration yielded comparable results, while the Salivette (R) method did not appear suitable for untargeted analysis of the salivary metabolome.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Jingjing Li, Donghao Zhang, Lingqian Yin, Zhiqiang Li, Chunlin Yu, Huarui Du, Xiaosong Jiang, Chaowu Yang, Yiping Liu
Summary: This study investigated the chemical composition of chicken meat during different growth and development periods using metabolomics. The results revealed dynamic alterations in metabolite composition with age, with various metabolic pathways affected. Integration analysis of metabolome and transcriptome data provided insights into the biological processes underlying meat quality development.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Applied
Feng Xu, Yuanyuan Pang, Qixing Nie, Zhipeng Zhang, Chuan Ye, Changtao Jiang, Yuan Wang, Huiying Liu
Summary: Pyrimidines are essential nutrients and key molecules in nucleic acid biosynthesis and metabolism. The concept of pyrimidine metabolome was proposed, covering 14 analytes and establishing a novel analytical strategy. Metabolomics analysis revealed diverse pyrimidine metabolism and offered reference data for future studies.
Article
Plant Sciences
Kun Zhang, Jing Ma, Sunil S. Gangurde, Lei Hou, Han Xia, Nana Li, Jiaowen Pan, Ruizheng Tian, Huailing Huang, Xingjun Wang, Yindong Zhang, Chuanzhi Zhao
Summary: In this study, metabolite profiling was performed on four peanut germplasm lines with different testa colors. A total of 85 metabolites were identified, and 78 differentially accumulated metabolites were found. The results showed significant differences in the accumulation of metabolites in testa with different colors, providing valuable information for further investigation of biosynthesis and accumulation mechanisms of these metabolites in peanut.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Endocrinology & Metabolism
Lihua Yao, Qin Wang, Runjie Zhang, Xingyun Wang, Yiwen Liu, Fangfang Di, Liwen Song, Siliang Xu
Summary: This study identified differential metabolites in BAT transplantation that target key signaling pathways associated with PCOS, suggesting metabolome changes play a vital role in improving PCOS. Sphingosine, a differential metabolite, accelerated apoptosis in KGN cells, indicating its potential as a therapeutic target for PCOS treatment.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Chemistry, Analytical
Artemis Lioupi, Maria Marinaki, Christina Virgiliou, Olga Begou, Helen Gika, Ian Wilson, Georgios Theodoridis
Summary: Metabolomics is an interdisciplinary field with applications in various areas, involving the analysis of metabolites in pathways such as glycolysis, amino acid metabolism, and the Krebs cycle. The determination of polar metabolites poses major challenges for chromatographic analysis, and existing methods are unable to comprehensively profile the polar metabolome. This review summarizes current approaches, advances, and trends in using liquid chromatography/mass spectrometry to address the instrumental and intellectual challenges involved in mapping the polar metabolome.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hassan Shikshaky, Eman Abdelnaby Ahmed, Ali Mostafa Anwar, Aya Osama, Shahd Ezzeldin, Antony Nasr, Sebaey Mahgoub, Sameh Magdeldin
Summary: This article aims to develop a novel SWATH platform for metabolite identification using accurate mass spectral library, providing new avenues for clinical diagnosis, molecular medicine, and therapeutic drug monitoring and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Yuan-Chih Chen, Hsin-Yi Wu, Chih-Wei Chang, Pao-Chi Liao
Summary: Conjugation reactions play a critical role in human metabolism, but identifying conjugated metabolites is challenging. In this study, a strategy called PDMS2E-DIA was proposed for comprehensive profiling of the glucuronide-conjugated metabolome. It successfully identified a large number of glucuronide-conjugated metabolites in human urine samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Chu-Fan Wang, Liang Li
Summary: In this study, a segmented spectrum scan method using Orbitrap MS was developed to improve the efficiency of metabolite detection in CIL LC-MS. The optimal settings for the segment scan were determined by examining various experimental conditions. The results showed that the segment scan could increase the number of detectable metabolites while slightly reducing the relative quantification accuracy.
ANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Yingting Zhang, Liwei Yang, Junjie Yang, Hailiang Hu, Guangqian Wei, Jiebing Cui, Jin Xu
Summary: The metabolite contents of Chinese cedar needles vary across different seasons. This study performed integrated non-targeted metabolomic and transcriptomic analyses to uncover the biosynthetic mechanisms of differentially synthesized metabolites (DSMs) in Chinese cedar needles. The results provide valuable insights into the adaptive mechanisms and extraction of metabolites in Chinese cedar.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Kai Uchida, June-Sik Kim, Muneo Sato, Hiromitsu Tabeta, Keiichi Mochida, Masami Yokota Hirai
Summary: This study used metabolomic data obtained by liquid chromatography-mass spectrometry to perform a metabolome GWAS and identified SNPs highly associated with metabolite contents in Arabidopsis thaliana seeds. The study also discovered an unknown methyltransferase gene associated with N-methylhistidine content, and confirmed its importance in N-methylhistidine biosynthesis through knockout and overexpression experiments.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agricultural Engineering
Sainan Ma, Ruchang Hu, Jieyu Ma, Jinwan Fan, Feifei Wu, Yang Wang, Linkai Huang, Guangyan Feng, Dandan Li, Gang Nie, Xinquan Zhang
Summary: Anthocyanin and proanthocyanidin are natural plant products beneficial to human and livestock health. Comparative transcriptomics and metabolomics analyses revealed differentially expressed genes and potential metabolites involved in anthocyanin and proanthocyanidin biosynthesis in white clovers. The findings provide a molecular genetic basis for future validation of candidate genes and breeding of high-proanthocyanidin white clover cultivars.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Atsushi Kimura, Akiyoshi Hirayama, Tatsuaki Matsumoto, Yuiko Sato, Tami Kobayashi, Satsuki Ikeda, Midori Maruyama, Mari Kaneko, Mayo Shigeta, Eri Ito, Tomoya Soma, Kana Miyamoto, Tomoyoshi Soga, Masaru Tomita, Akihito Oya, Morio Matsumoto, Masaya Nakamura, Arihiko Kanaji, Takeshi Miyamoto
Summary: Ossification of the posterior longitudinal ligament (OPLL), a disease characterized by ectopic ossification of a spinal ligament, can lead to neurological disorders. This study investigated the role of hydroxyacid oxidase 1 (Hao1) in OPLL development and found that Hao1 expression decreased during osteoblast differentiation. Further experiments using Hao1-deficient mice showed that Hao1 loss did not promote ectopic ossification, but rather regulated the tricarboxylic acid (TCA) cycle metabolism.
Review
Biochemistry & Molecular Biology
Serafettin Gunes, Yumi Aizawa, Takuma Sugashi, Masahiro Sugimoto, Pedro Pereira Rodrigues
Summary: Early diagnosis is crucial for delaying the onset of Alzheimer's disease, and current diagnostic methods are invasive and expensive, highlighting the need for more convenient and effective biomarkers. Studies have identified potential biomarkers, but further validation research is necessary.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yukako Suzuki, Ryosuke Hayasaka, Masako Hasebe, Satsuki Ikeda, Tomoyoshi Soga, Masaru Tomita, Akiyoshi Hirayama, Hiroki Kuroda
Summary: This study identified specific small molecules expressed in the dorsal or ventral marginal zones in vertebrate gastrula, investigating their impact on cell fate. By analyzing hydrophilic metabolites and lipids, the research found differences in metabolism-related molecules between the two regions, contributing to a better understanding of cellular physiology during early embryogenesis.
Article
Biochemistry & Molecular Biology
Yoji Saeki, Akane Takenouchi, Etsuyo Otani, Minji Kim, Yumi Aizawa, Yasuko Aita, Atsumi Tomita, Masahiro Sugimoto, Takashi Matsukubo
Summary: This study evaluated the effect of long-term mastication on salivary metabolomic profiles and found that most metabolites showed lower concentrations after chewing. The pathways related to glycogenic amino acids also underwent significant changes.
Article
Gastroenterology & Hepatology
Taisuke Matsumoto, Masakatsu Fukuzawa, Takao Itoi, Masahiro Sugimoto, Yumi Aizawa, Makoto Sunamura, Takashi Kawai, Daiki Nemoto, Hirokazu Shinohara, Takahiro Muramatsu, Yuka Suzuki, Yasuyuki Kagawa, Maya Suguro, Kumiko Uchida, Yohei Koyama, Akira Madarame, Takashi Morise, Hayato Yamaguchi, Akihiko Sugimoto, Yoshiya Yamauchi, Shin Kono, Sakiko Naito
Summary: This study analyzed plasma metabolomics and identified a combination of quantified metabolites that showed high sensitivity for the detection of gastric cancer (GC). These metabolites were found to be independent of commonly used tumor markers, suggesting that their complementary use could be helpful for GC screening.
Article
Biochemistry & Molecular Biology
Daisuke Nose, Masahiro Sugimoto, Tsuneo Muta, Shin-Ichiro Miura
Summary: Pancreatic cancer is a highly malignant cancer with a poor prognosis. It is often diagnosed late due to the absence of typical symptoms. Therefore, it is necessary to establish a screening method for early detection of pancreatic cancer in high-risk individuals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Yoshie Nakayama, Mariko Hanada, Hiroshi Koda, Masahiro Sugimoto, Masahiro Takada, Masakazu Toi
Summary: This study aimed to evaluate volatile compounds in exhaled breath as a non-invasive screening method to detect breast neoplasms. After analyzing breath samples, significant differences in volatile compounds were found between patients with breast cancer and non-breast cancer controls. The developed MLR model can effectively discriminate between breast cancer and non-breast cancer samples. This study suggests the potential of volatile compounds in exhaled breath as a noninvasive screening method for breast cancer.
JOURNAL OF BREATH RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Akiyoshi Hirayama, Takamasa Ishikawa, Haruka Takahashi, Sanae Yamanaka, Satsuki Ikeda, Aya Hirata, Sei Harada, Masahiro Sugimoto, Tomoyoshi Soga, Masaru Tomita, Toru Takebayashi
Summary: High-throughput metabolomics has facilitated large-scale cohort studies. Long-term studies require multiple batch-based measurements, which necessitates sophisticated quality control to eliminate bias. In this study, liquid chromatography-mass spectrometry was used to analyze 10,833 samples in 279 batch measurements. The quantified profile included 147 lipids. Normalization using QC samples reduced the coefficient of variation for the lipids. This study provides unbiased and quantified data for large-scale metabolomics.
Article
Oncology
Sachika Shiraishi, Masahiro Sugimoto, Koichi Tokuuye
Summary: The study evaluated salivary metabolomics as an independent predictor for radiation pneumonitis, identifying three metabolites with predictive ability for clinical RP.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Chemistry, Medicinal
Hiroshi Arakawa, Takumi Kawanishi, Dai Shengyu, Takumi Nishiuchi, Makiko Meguro-Horike, Shin-ichi Horike, Masahiro Sugimoto, Yukio Kato
Summary: This study investigates the effects of hepatobiliary cholestasis on renal exposure to nephrotoxic drugs. The results show that tissue accumulation of imatinib and cisplatin in kidney slices is higher in mice with bile duct ligation (BDL) compared to sham-operated mice. Proteomic analysis reveals a reduction in the expression of the efflux transporter Mrp6 in the kidneys of BDL mice. These findings suggest that cholestasis leads to drug accumulation in renal cells and promotes drug-induced renal injury.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Daisuke Nose, Tomokazu Matsui, Takuya Otsuka, Yuki Matsuda, Tadaaki Arimura, Keiichi Yasumoto, Masahiro Sugimoto, Shin-Ichiro Miura
Summary: A study developed a model to estimate pleural effusion in heart failure patients using multi-frequency bioelectrical impedance analysis, which showed high accuracy in discriminating patients with pleural effusion. This approach offers a potential clinical application for assessing intrathoracic conditions.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Article
Health Care Sciences & Services
Masahiro Sugimoto, Atsumi Tomita, Michiko Oyamada, Mitsue Sato
Summary: The gaze trajectories of nurses with different levels of experience were monitored during an intravenous injection task. The results showed that experienced nurses had different patterns of gaze duration compared to nursing students. A state transition diagram visualized the recognition errors and repeated confirmation behaviors. Clustering analysis of gaze durations also indicated similarity among participants with similar experiences.
Article
Biochemical Research Methods
Ryuhei Kudo, Kaori Igarashi, Tomoyoshi Soga, Takamasa Ishikawa, Yasuhiro Saito
Summary: Capillary electrophoresis mass spectrometry (CE-MS) and liquid chromatography mass spectrometry (LC-MS) are two commonly used methods for metabolite analysis, but require independent sample preparation. This article presents a sample preparation protocol for breast cancer cell lines.
Article
Multidisciplinary Sciences
Misa Yoshizawa, Masahiro Sugimoto, Minoru Tanaka, Yusuyuki Sakai, Masaki Nishikawa
Summary: Liver fibrosis is a result of homeostasis breakdown caused by repetitive injury, leading to collagen accumulation that disrupts liver structure and function, resulting in serious consequences such as cirrhosis. Study using mathematical simulation model found accumulation of collagens from different sources in different regions of the liver, providing insights into a new mechanism for understanding liver fibrosis.
SCIENTIFIC REPORTS
(2022)
