Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 58, Issue 7, Pages 4051-4055Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf903658k
Keywords
ctx gene; culture fish; real-time PCR; Vibrio cholerae
Funding
- Xunta de Galicia (Spain) [08TAL003CT, 09TAL002CT]
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The main objective of the present work was to evaluate a real-time polymerase chain reaction (FOR) method to detect toxigenic Vibrio cholerae in Pangasius hypophthalmus, a freshwater fish cultured mainly in South East Asia. A FDA traditional culture method and a real-time FOR method of the ctx gene were used for detection of V. cholerae in spiked samples of pangasius fish. After an overnight enrichment of samples at 37 degrees C in alkaline peptone water, 2 cfu/25 g of fish was detected with both methods. Although both methods were very sensitive, obtaining results with culture methods may take several days, while real-time PCR takes only a few hours. Furthermore, with traditional methods, complementary techniques such as serotyping, although not available for all serogroups, are needed to identify toxigenic V. cholerae. However, with real-time PCR, toxigenic serogroups are detected in only one step after overnight enrichment.
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