Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 58, Issue 20, Pages 10876-10879Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf1028416
Keywords
(+)-Osbeckic acid; tartary buckwheat; vasorelaxation; aorta
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The candidates responsible for vasorelaxation action of rutin-free tartary buckwheat extract (TBSP) were examined in this study. As a result of reversed-phase high-performance liquid chromatography (HPLC) separations, five prominent peaks in the acidic fraction of TBSP were obtained at 260 nm. Among the five collected peaks, we successfully identified four compounds by nuclear magnetic resonance (NMR) and mass spectrometry (MS) measurements: (+)-osbeckic acid as a dimer ([M -H](-) m/z 371.2 >184.9 > 140.9), 5-hydroxymethyl-2-furoic acid, protocatechuic acid, and p-hydroxybenzoic acid. A vascular contractive measurement in 1.0 mu M phenylephrine-contracted Sprague Dawley rat thoracic aorta rings revealed that (+)-osbeckic acid dimer evoked a potent vasorelaxant effect with an EC(50) value of 887 mu M compared to other isolates (EC(50): 5-hydroxymethyl-2-furoic acid, 3610 mu M; protocatechuic acid, 2160 mu M; p-hydroxybenzoic acid, no inhibition). Dimeric (+)-osbeckic acid was stable in solutions and at high temperatures, while its degraded peak on the HPLC chromatogram was observed when it was dissolved in dimethyl sulfoxide.
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