Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 58, Issue 10, Pages 6096-6101Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf100414v
Keywords
Miraculin; transgenic tomato; artificial light; closed cultivation system; mass production
Funding
- Grants-in-Aid for Scientific Research [11J40033] Funding Source: KAKEN
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We constructed a cultivation system with a controlled light period, light intensity, temperature, and CO2 concentration for mass production of the taste-modifying protein miraculin from transgenic tomatoes. The tomato plants exhibited normal growth and produced over 270 g of fresh weight (FW) fruit per plant, with the recombinant miraculin concentration reaching up to 90 mu g per g FW of tomatoes. The recombinant miraculin content of transgenic tomatoes was compared to that of plants grown in a netted greenhouse. The recombinant miraculin content of transgenic tomatoes grown in a closed cultivation system was more stable than that of tomatoes grown in a netted greenhouse, suggesting that the closed cultivation system is suitable for the production of recombinant miraculin. We estimate that 45 tFW of tomatoes and 4 kg of recombinant miraculin per 1,000 m(2) of cultivation area can be harvested per year.
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