Extensive Dry Ball Milling of Wheat and Rye Bran Leads to in Situ Production of Arabinoxylan Oligosaccharides through Nanoscale Fragmentation

This study investigated the potential of ball milling as a dry process for in situ production of (AXOS) in arabinoxylan (AX)-rich wheat and rye bran. An extensive (120 h, 50% jar volume capacity) increased the wheat bran water(WE-AX) level from 4% (untreated bran) to 61% of the wheat bran AX. (M of 15 kDa. Ball milling of rye bran gave rise to similar results, with the A/X ratio of the (0.51). Optimization of the ball mill treatment by varying the (>70%) in wheat (24 h, 16% jar Volume yielded wheat bran AXOS, with an A/X ratio of 0.65 and a MM of 6 kDa. Ball milling (24 h,) of pericarp-enriched wheat bran increased the WE-AX level from 1% (untreated pericarp) to 63%. The extractable material had a high A/X ratio (0.97) and a low MM (5 Fluorescence microscopy revealed that the extensive ball mill treatment led to the almost in situ from wheat or rye bran in a