Article
Food Science & Technology
Miku Akishino, Yuko Aoki, Hajime Baba, Makio Asakawa, Yoichiro Hama, Susumu Mitsutake
Summary: This study investigates the interaction between Isofloridoside (IF) and the sweet taste receptor T1R2/T1R3, showing that IF interacts with T1R2/T1R3 and has the potential as a new alternative sweetener.
Article
Biochemistry & Molecular Biology
Congrui Wang, Yi Liu, Meng Cui, Bo Liu
Summary: Sweet taste is crucial for the preference and adaption of primates to diet. It is mediated by a GPCR-sweet taste receptor T1R2/T1R3. This study provides new insights into the molecular determinants of sweet taste recognition in primates.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kristian Daly, Andrew W. Moran, Miran Al-Rammahi, Darren Weatherburn, Soraya P. Shirazi-Beechey
Summary: This study demonstrates notable differences among species in the ability to detect certain non-nutritive sweeteners, attributed to amino acid substitutions and pseudogenization of taste receptor genes. Using pig models, it was shown that commonly used non-nutritive sweeteners such as sucralose, saccharin, and acesulfame K activate the T1R2-T1R3 receptor, while aspartame and cyclamate do not.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Agriculture, Dairy & Animal Science
Momoko Higashida, Yuta Yoshida, Yuko Kawabata, Yuki Matsui, Shotaro Nishimura, Shoji Tabata, Fuminori Kawabata
Summary: Investigating the sweet-sensing systems in chickens revealed that they can sense various sweet compounds through T1R2-independent pathways in the palate tissues. This research has important implications for improving poultry nutrition and feeding strategies in poultry farming.
Article
Biochemistry & Molecular Biology
Ken Kobayashi, Liang Han, Taku Koyama, Shan-Ni Lu, Takanori Nishimura
Summary: A sweet taste receptor subunit T1R3 was found in both the apical and basolateral membranes of mammary epithelial cells (MECs). In a cell culture model, sucralose on both the apical and basolateral membranes activated the sweet taste receptor, leading to increased phosphorylation of STAT5, which is involved in milk production.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Behavioral Sciences
Nitzan Dubovski, Yaron Ben-Shoshan Galeczki, Einav Malach, Masha Y. Niv
Summary: We have found that L-glucose, the non-caloric enantiomer of D-glucose, can activate the human sweet taste receptor T1R2/T1R3 transiently expressed in HEK293T cells. Both D-glucose and L-glucose can also activate T1R2 and T1R3 without their counterpart monomer. Mutation of residue 147 in the binding site of T1R3 VFT domain abolishes activation by L- or D-glucose, while T1R2/T1R3S147A responds similarly to T1R2 alone. We have also discovered that the original T1R2 reference sequence is less sensitive compared to the reference sequence at the time of the study, with a specific mutation in the VFT domain responsible for this effect.
Article
Chemistry, Physical
Wilfredo Evangelista-Falcon, Clement Denhez, Angelica Baena-Moncada, Miguel Ponce-Vargas
Summary: It is widely accepted that sweet taste perception is activated by the sweet taste receptor (STR), a complex of T1R2 and T1R3. However, the interaction between STR and sweeteners is not fully understood. In this study, a combination of molecular dynamics and the independent gradient model (igm) approach was used to analyze the interaction between the T1R2 Venus flytrap domain (VFD) and aspartame. The results provide insights into the key interactions that stabilize the closed form of VFD and can be applied to the design of new sweeteners.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Anatomy & Morphology
Jea Hwa Jang, Ha Kyeong Kim, Dong Woo Seo, Su Young Ki, Soonhong Park, Sang-Hyun Choi, Dong-Hoon Kim, Seok Jun Moon, Yong Taek Jeong
Summary: Research has shown that sweet taste receptors have unknown functions in the brain and may serve as a novel therapeutic target in the central nervous system. Using T1r2-Cre knock-in mice, the expression pattern of T1R2 in the brain was revealed. Sweet taste receptors are not only expressed in neurons, but also in glial populations in circumventricular organs and vascular structures in the brain.
FRONTIERS IN NEUROANATOMY
(2021)
Article
Multidisciplinary Sciences
Fabio Zani, Julianna Blagih, Tim Gruber, Michael D. Buck, Nicholas Jones, Marc Hennequart, Clare L. Newell, Steven E. Pilley, Pablo Soro-Barrio, Gavin Kelly, Nathalie M. Legrave, Eric C. Cheung, Ian S. Gilmore, Alex P. Gould, Cristina Garcia-Caceres, Karen H. Vousden
Summary: Artificial sweeteners, calorie-free substitutes for sugar, are widely used in many food products. However, concerns have been raised about the long-term safety of consuming certain sweeteners. This study shows that high doses of sucralose can have immunomodulatory effects, limiting T cell proliferation and differentiation, affecting membrane order, T cell receptor signaling, and intracellular calcium mobilization. Mice given sucralose showed decreased T cell responses in cancer and bacterial infection models, as well as reduced T cell function in autoimmune models. These findings suggest that sucralose intake can dampen T cell-mediated responses, which may be useful in treating T cell-dependent autoimmune disorders.
Article
Multidisciplinary Sciences
Hengwu Jiao, Huan-Wang Xie, Libiao Zhang, Nima Zhuoma, Peihua Jiang, Huabin Zhao
Summary: Despite the high conservation of sweet taste receptor genes between frugivorous and insectivorous bats at the sequence level, there is a significant functional divergence, as evidenced by behavioral experiments and cell-based assays. Frugivorous bats exhibited a strong preference for natural sugars, while insectivorous bats did not, indicating a close relationship between the functional evolution of sweet taste receptors and diet. This study highlights the importance of combining different approaches in molecular evolutionary studies and cautions against relying solely on sequence conservation for inferring protein and physiological function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Tomoya Nakagita, Takumi Matsuya, Masataka Narukawa, Takuya Kobayashi, Takatsugu Hirokawa, Takumi Misaka
Summary: This study created a docked model of the transmembrane domain (TMD) of the T1R3 subunit of the human sweet taste receptor with neohesperidin dihydrochalcone (NHDC) and identified important residues for NHDC recognition. The docking mode of NHDC with T1R3 TMD was distinct from that of lactisole, a sweet taste inhibitor.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Psychology, Biological
Ezen Choo, Anna Koh, Jason Goodman, Jennifer Bushnell, Hanna Mielke-Maday, Bryan Merte, Robin Dando
Summary: During pregnancy, maternal taste preferences change, likely due to alterations in taste buds' expression profile, which may promote a less healthy diet while expecting.
PHYSIOLOGY & BEHAVIOR
(2021)
Article
Endocrinology & Metabolism
Yosuke Masubuchi, Jinhui Ma, Tomohiro Suzuki, Itaru Kojima, Takeshi Inagaki, Hiroshi Shibata
Summary: T1R3, expressed in adipocytes, negatively regulates insulin-induced glucose transport via Gαs-dependent microtubule disassembly. Activation of T1R3 also inhibits insulin-induced GLUT4 translocation to the plasma membrane.
Article
Biology
Nanako Atsumi, Keiko Yasumatsu, Yuriko Takashina, Chiaki Ito, Norihisa Yasui, Robert F. Margolskee, Atsuko Yamashita
Summary: The perception of salt taste is complex, with NaCl at low and high concentrations being preferentially or aversively perceived through different pathways. Cl- is believed to participate in taste sensation through an unknown mechanism. This study found that Cl- ions specifically bind to the ligand-binding domain (LBD) of taste receptor type 1 (T1r), inducing a conformational change in the receptor. The oral application of Cl- to mice increased the activity of taste nerves connected to T1r-expressing taste cells and influenced their behavioral preferences, which could be attenuated by blocking T1r or knocking out T1r3.
Review
Agriculture, Multidisciplinary
Shulei Zhao, Hanyuan Zheng, Yujia Lu, Na Zhang, Olugbenga P. Soladoye, Yuhao Zhang, Yu Fu
Summary: The long-term consumption of a high-sugar diet can lead to the development of chronic diseases like obesity and type 2 diabetes. Sweet peptides, derived from various food sources, can enhance the sweet taste while maintaining sensory properties. Therefore, the research and application of sweet peptides are promising strategies for reducing sugar consumption.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Takanori Nakane, Changyong Song, Mamoru Suzuki, Eriko Nango, Jun Kobayashi, Tetsuya Masuda, Shigeyuki Inoue, Eiichi Mizohata, Toru Nakatsu, Tomoyuki Tanaka, Rie Tanaka, Tatsuro Shimamura, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, So Iwata, Michihiro Sugahara
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Kaho Nishizawa, Tetsuya Masuda, Yasuyuki Takenaka, Hironori Masui, Fumito Tani, Yasuhiro Arii
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2016)
Review
Food Science & Technology
Tetsuya Masuda
JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI
(2016)
Article
Multidisciplinary Sciences
Yohta Fukuda, Ka Man Tsea, Takanori Nakane, Toru Nakatsu, Mamoru Suzuki, Michihiro Sugahara, Shigeyuki Inoue, Tetsuya Masuda, Fumiaki Yumoto, Naohiro Matsugaki, Eriko Nango, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Changyong Song, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Michael E. P. Murphy, Tsuyoshi Inoue, So Iwata, Eiichi Mizohata
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Multidisciplinary Sciences
Takanori Nakane, Shinya Hanashima, Mamoru Suzuki, Haruka Saiki, Taichi Hayashi, Keisuke Kakinouchi, Shigeru Sugiyama, Satoshi Kawatake, Shigeru Matsuoka, Nobuaki Matsumori, Eriko Nango, Jun Kobayashi, Tatsuro Shimamura, Kanako Kimura, Chihiro Mori, Naoki Kunishima, Michihiro Sugahara, Yoko Takakyu, Shigeyuki Inoue, Tetsuya Masuda, Toshiaki Hosaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Tsuyoshi Inoue, Osamu Nureki, So Iwata, Michio Murata, Eiichi Mizohata
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Multidisciplinary Sciences
Tetsuya Masuda, Keisuke Ohta, Naoko Ojiro, Kazuki Murata, Bunzo Mikami, Fumito Tani, Piero Andrea Temussi, Naofumi Kitabatake
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Michihiro Sugahara, Changyong Song, Mamoru Suzuki, Tetsuya Masuda, Shigeyuki Inoue, Takanori Nakane, Fumiaki Yumoto, Eriko Nango, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, So Iwata
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Michihiro Suga, Fusamichi Akita, Michihiro Sugahara, Minoru Kubo, Yoshiki Nakajima, Takanori Nakane, Keitaro Yamashita, Yasufumi Umena, Makoto Nakabayashi, Takahiro Yamane, Takamitsu Nakano, Mamoru Suzuki, Tetsuya Masuda, Shigeyuki Inoue, Tetsunari Kimura, Takashi Nomura, Shinichiro Yonekura, Long-Jiang Yu, Tomohiro Sakamoto, Taiki Motomura, Jing-Hua Chen, Yuki Kato, Takumi Noguchi, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Eriko Nango, Rie Tanaka, Hisashi Naitow, Yoshinori Matsuura, Ayumi Yamashita, Masaki Yamamoto, Osamu Nureki, Makina Yabashi, Tetsuya Ishikawa, So Iwata, Jian-Ren Shen
Article
Multidisciplinary Sciences
Tetsuya Masuda, Mamoru Suzuki, Shigeyuki Inoue, Changyong Song, Takanori Nakane, Eriko Nango, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Bunzo Mikami, Osamu Nureki, Keiji Numata, So Iwata, Michihiro Sugahara
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Tetsuya Masuda, Bunzo Mikami, Fumito Tani
Article
Biochemical Research Methods
Michihiro Sugahara, Eiichi Mizohata, Eriko Nango, Mamoru Suzuki, Tomoyuki Tanaka, Tetsuya Masudala, Rie Tanaka, Tatsuro Shimamura, Yoshiki Tanaka, Chiyo Suno, Kentaro Ihara, Dongqing Pan, Keisuke Kakinouchi, Shigeru Sugiyama, Michio Murata, Tsuyoshi Inoue, Kensuke Tono, Changyong Song, Jaehyun Park, Takashi Kameshima, Takaki Hatsui, Yasumasa Joti, Makina Yabashi, So Iwata
Article
Multidisciplinary Sciences
Michihiro Sugahara, Takanori Nakane, Tetsuya Masuda, Mamoru Suzuki, Shigeyuki Inoue, Changyong Song, Rie Tanaka, Toru Nakatsu, Eiichi Mizohata, Fumiaki Yumoto, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, Eriko Nango, So Iwata
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Tetsuya Masuda, Kyohei Okubo, Kazuki Murata, Bunzo Mikami, Michihiro Sugahara, Mamoru Suzuki, Piero Andrea Temussi, Fumito Tani
Article
Multidisciplinary Sciences
Michihiro Sugahara, Koji Motomura, Mamoru Suzuki, Tetsuya Masuda, Yasumasa Joti, Keiji Numata, Kensuke Tono, Makina Yabashi, Tetsuya Ishikawa
SCIENTIFIC REPORTS
(2020)
Article
Biochemical Research Methods
Takeshi Murakawa, Mamoru Suzuki, Kenji Fukui, Tetsuya Masuda, Michihiro Sugahara, Kensuke Tono, Tomoyuki Tanaka, So Iwata, Eriko Nango, Takato Yano, Katsuyuki Tanizawa, Toshihide Okajima
Summary: Recent progress in understanding the mechanisms by which enzymes promote catalytic reactions efficiently through their structural changes has been made using serial femtosecond X-ray crystallography and mix-and-inject serial crystallography techniques. In this study, researchers successfully observed the structural changes associated with ongoing enzymic reactions using a liquid-jet system. The results indicate that anaerobic conditions were maintained throughout the measurements, preventing interference with the catalytic intermediates.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2022)
