Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 56, Issue 16, Pages 7231-7235Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf800748p
Keywords
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Funding
- PAI Excellence program [FQM-1515]
- Fundacao para a Ciencia e Tecnologia [SFRH/BD/28333/2006]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/28333/2006] Funding Source: FCT
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A continuous approach assisted by ultrasound for direct enrichment of edible oils (olive, sunflower, and soya) with the main phenols in olive leaves (i.e., oleuropein, verbascoside, apigenin-7-glucoside, and luteolin-7-glucoside) has been developed. Multivariate methodology was used to carry out a detailed optimization of the enrichment, and quantitation of the transferred compounds was based on LC-MS-MS in multiple reaction monitoring optimizing the most sensitive transition for each biophenol. Under the optimal working conditions, only 20 min is necessary to enrich the edible oils with 14.45-9.92 mu g/mL oleuropein, 2.29-2.12 mu g/mL verbascoside, 1.91-1.51 mu g/ mL apigenin-7-glucoside, and 1.60-1.42 mu g/mL luteolin-7-glucoside. The enrichment method is carried out at room temperature and is organic-solvent-free; thus, the healthy properties of the edible oils improve as does their quality. Also, the low acquisition and maintenance costs of an ultrasound source and its application in a dynamic system make advisable the industrial implementation of the proposed method.
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