Article
Biochemistry & Molecular Biology
Maura Brioschi, Erica Gianazza, Piergiuseppe Agostoni, Beatrice Zoanni, Alice Mallia, Cristina Banfi
Summary: This study used a targeted mass spectrometry approach to analyze plasma samples from HF patients and healthy controls, identifying a panel of four plasma proteins associated with HF severity and pulmonary dysfunction, with the potential to distinguish between HF patients and healthy subjects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Claudia Gaither, Robert Popp, Sophia P. Borchers, Kjartan Skarphedinsson, Finnur F. Eiriksson, Margret Thorsteinsdottir, Yassene Mohammed, Christoph H. Borchers
Summary: The study demonstrated that leaving a lyophilized peptide mixture at room temperature for up to 20 days does not affect the quantitative performance of mass spectrometry analysis, making it a feasible option for sample transportation. Most peptide concentrations showed minimal fluctuations across different time points, indicating the stability of the peptide mixture under the tested conditions.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Raymond Neil Dalton, Timothy Scott Isbell, Ryan Ferguson, Louis Fiore, Andrei Malic, Jeffrey Anton DuBois
Summary: Early detection of CKD using point of care creatinine and eGFR testing improves patient management outcomes. A study was conducted to evaluate the use of a whole blood creatinine/eGFR device in a rural Nicaraguan population. Results showed a good correlation between all creatinine methods and the gold standard ID-LCMS method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Akash Kumar Bhaskar, Salwa Naushin, Arjun Ray, Praveen Singh, Anurag Raj, Shalini Pradhan, Khushboo Adlakha, Towfida Jahan Siddiqua, Dipankar Malakar, Debasis Dash, Shantanu Sengupta
Summary: Lipid compositions are complex and challenging to identify, necessitating new comprehensive analysis methods. This study proposes a targeted-mass spectrometry method that can rapidly screen a large number of lipid species, and identifies alterations in plasma lipids due to vitamin B(12) deficiency.
Article
Biochemical Research Methods
Dongyoon Shin, Sang Jin Rhee, Jihyeon Lee, Injoon Yeo, Misol Do, Eun-Jeong Joo, Hee Yeon Jung, Sungwon Roh, Sang-Hyuk Lee, Hyeyoung Kim, Minji Bang, Kyu Young Lee, Jun Soo Kwon, Kyooseob Ha, Yong Min Ahn, Youngsoo Kim
Summary: This study successfully differentiated major depressive disorder (MDD) from bipolar disorder (BD) using a targeted proteomic approach, developing a model composed of nine proteins with good discriminative ability. The proteins identified were associated with neuro, oxidative/nitrosative stress, and immunity/inflammation-related biological functions.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Rossella Barone, Matteo Conti, Pier Giorgio Cojutti, Milo Gatti, Pierluigi Viale, Federico Pea
Summary: Dalbavancin (DBV) is a long-acting antistaphylococcal lypoglycopeptide increasingly used for long-term treatment of subacute and/or chronic infections. Population pharmacokinetic studies have shown that two 1500mg doses of DBV 1 week apart can effectively treat infections for weeks. This study developed and validated a fast and simple analytical method for measuring DBV concentrations in human plasma microsamples using Liquid Chromatography-Isotope Dilution Tandem Mass Spectrometry (ITD LC-MS/MS) technique.
Article
Chemistry, Analytical
C. Venchiarutti, G. Stadelmann, R. Middendorp, Z. Macsik, A. Venzinb
Summary: In this study, picogram amounts of uranium in particle form were quantified using optical microscopy combined with Isotope Dilution Thermal Ionisation Mass Spectrometry (ID-TIMS). The uranium amount was verified using Isotope Dilution Mufti Collection Inductively Coupled Plasma Mass Spectrometry (ID-MC-ICPMS), and both techniques showed good agreement within uncertainties. The uranium particle material in this study was eventually certified for the uranium isotopic composition of particles and the uranium amount per particle, proving the suitability of the techniques for quantifying picogram amounts of uranium in particulate reference materials.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Huu-Hien Huynh, Amandine Boeuf, Maxence Derbez-Morin, Anne-Marie Dupuy, Beatrice Lalere, Vincent Delatour, Joelle Vinh
Summary: An optimized method for quantifying PCT in human serum at sub-microgram per liter level was developed using LC-MS/MS, with linearity established through peptide-based calibration curves and accuracy evaluated using quality control materials. A correction factor was applied to compensate for digestion incompleteness and material loss, enabling accurate quantification with results traceable to SI units. The method showed a twofold lower PCT concentration compared to immunoassay results when applied to serum samples from sepsis patients.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Gabriella Pinto, Michela Aurilia, Anna Illiano, Carolina Fontanarosa, Giovanni Sannia, Marco Trifuoggi, Vincenzo Lettera, Raffaele Sperandeo, Piero Pucci, Angela Amoresano
Summary: This study conducted a preliminary untargeted metabolic screening and a targeted quantification of polyphenols using LC-MS/MS, which found that chocolate made from Ecuador cocoa beans had the highest level of polyphenols.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Hong Liu, Cheng Yang Ng, Qinde Liu, Tang Lin Teo, Tze Ping Loh, Moh Sim Wong, Sunil Kumar Sethi, Jun Guan Tan, Ping Ying Heng, Sharon Saw, Leslie Choong-Weng Lam, Joanne Mee-Yin Lee, Khairee Bin Khaled, Hnin Pwint Phyu, Noel Wan Ting Ong
Summary: Urine albumin concentration and albumin-creatinine ratio are crucial for early-stage kidney damage screening. Commutable urine certified reference materials (CRMs) for albumin and creatinine are essential for standardization and accurate measurement. This study evaluated the commutability of two urine CRMs on five different clinical analyzers, showing satisfactory results for both albumin and creatinine measurements, indicating their suitability for routine use in clinical laboratories.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Infectious Diseases
Rossella Barone, Matteo Conti, Pier Giorgio Cojutti, Milo Gatti, Pierluigi Viale, Federico Pea
Summary: In this study, a fast and sensitive LC-ITD-MS/MS method was developed and validated for measuring Cefiderocol (C) in human plasma microsamples as small as 3 microliters. The method was successfully validated based on EMA guidelines and was applied to therapeutic drug monitoring (TDM) of critically ill patients with carbapenem-resistant Gram-negative bacterial infections, optimizing antibiotic therapy.
Article
Biochemical Research Methods
Jorge Pitarch-Motellon, Lubertus Bijlsma, Juan Vicente Sancho Llopis, Antoni F. Roig-Navarro
Summary: The study showed that using Isotope Pattern Deconvolution (IPD) quantification method for determining substances in wastewater can reduce uncertainty in estimating illicit drug concentration, improving accuracy and reliability. Additionally, IPD is faster and cost-effective when dealing with high matrix effects, analyzing a large number of samples and compounds.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jianyi Liu, Wen Zhu, Haofeng Sun, Dewei Song, Peng Xiao, Bei Xu, Hongmei Li
Summary: The development of a certified reference material (CRM) for natural CRP, named GBW09228, was reported, with certification results traceable to SI units. This CRM can be used to calibrate and verify the accuracy of CRP measurements, providing a foundation for establishing a complete traceability chain for CRP.
ANALYTICAL METHODS
(2021)
Article
Medicine, Research & Experimental
Qingbo Shu, Shan Liu, Tonino Alonzi, Sylvia M. LaCourse, Dhiraj Kumar Singh, Duran Bao, Dalton Wamalwa, Li Jiang, Christopher J. Lyon, Grace John-Stewart, Deepak Kaushal, Delia Goletti, Tony Hu
Summary: Using mass spectrometry (MS) to read immunoassays for biomarker-derived peptides can improve sensitivity and specificity, allowing for better identification of tuberculosis cases and differentiation between disease and asymptomatic infection. This approach shows strong potential for infectious disease diagnosis and monitoring changes in Mycobacterium tuberculosis burden for more effective disease control.
Article
Chemistry, Analytical
Esther S. Breuninger, Julie Tolu, Sylvain Bouchet, Lenny H. E. Winkel
Summary: Although oceans play a crucial role in the global selenium cycle, there is limited quantitative information available on selenium concentrations and speciation in marine environments. This study developed a sensitive method using species-specific isotope dilution gas chromatography coupled to inductively coupled plasma mass spectrometry (ID-GC-ICPMS) to determine the different selenium chemical fractions in seawater, allowing for the measurement of selenium concentrations in brackish and marine systems.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Lucia Bartella, Marcos Bouza, Priscilla Rocio-Bautista, Leonardo Di Donna, Juan F. Garcia-Reyes, Antonio Molina-Diaz
Summary: Ambient mass spectrometry is an alternative to classical mass spectrometry that allows fast, in-situ, and high-throughput analysis of samples with minimal sample pretreatment. This study explores the capability of different ambient ionization sources to analyze raw wine for quality control purposes. The spectra obtained from these sources showed the ability to classify and differentiate wine samples based on various compounds, including organic acids, phenolic acids, derivatives, and low-molecular-mass flavonoids. The plasma-based methods offer the advantage of solventless analysis, while the paper spray (PS) approach provides a richer MS profile.
MICROCHEMICAL JOURNAL
(2022)
Review
Chemistry, Multidisciplinary
David Bongiorno, Giuseppe Avellone, Anna Napoli, Fabio Mazzotti, Daniela Piazzese, Valentina Censi, Serena Indelicato
Summary: The production of red wine is vital for the economies of many countries. Fining agents are commonly used to clarify and remove undesired substances in the winemaking process. However, these agents may pose a potential risk to allergic individuals. This review highlights the recent advancements in the quantification of fining agent residues.
FRONTIERS IN CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Valentina Censi, Filippo Saiano, David Bongiorno, Serena Indelicato, Anna Napoli, Daniela Piazzese
Summary: Although petroleum-based plastics have advantages in meeting performance requirements, they are associated with environmental problems throughout their life cycle. Therefore, reducing consumption and dependence on fossil-based materials is necessary to preserve natural resources and combat climate change. The use of biopolymers has grown exponentially due to improvements in their physical and mechanical properties. This review discusses different ways of biopolymer degradation and evaluates their potential in eco-sustainability. It also focuses on the quantitative analysis methods of bioplastics and their degradation by-products, comparing and evaluating the advantages and disadvantages of each technique.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Lucia Bartella, Fabio Mazzotti, Ines Rosita Talarico, Giuseppina De Luca, Ilaria Santoro, Mario Prejano, Costanza Riccioni, Tiziana Marino, Leonardo Di Donna
Summary: The structural characterization and antioxidant ability of a new flavonoid from bergamot fruit have been investigated. The new molecule, named peripolin, possesses strong antioxidant behavior and shows higher activity compared to other flavonoids present in the fruit. This study provides important insights into the antioxidant characteristics of bergamot fruit.
Article
Food Science & Technology
Antonella Muto, Sarah R. Christofides, Tiziana Maria Sirangelo, Lucia Bartella, Carsten Muller, Leonardo Di Donna, Innocenzo Muzzalupo, Leonardo Bruno, Antonio Ferrante, Adriana A. C. Chiappetta, Maria Beatrice Bitonti, Hilary J. Rogers, Natasha Damiana Spadafora
Summary: This study investigated the quality changes of six peach and nectarine cultivars during cold storage. The results showed that different cultivars responded differently to storage conditions, and sensory parameters were correlated with various compounds. Acidity and firmness were negatively correlated with mouthfeel and sweetness, while Brix value and β-carotene were positively correlated with sweetness and harmony.
Article
Chemistry, Multidisciplinary
Fabrizio Olivito, Vincenzo Algieri, Matteo Antonio Tallarida, Antonio Jiritano, Paola Costanzo, Loredana Maiuolo, Antonio De Nino
Summary: The conversion of naturally occurring organic substances into value-added platform chemicals by simple, green, and efficient procedures represents one of the most accessible and sought-after routes towards sustainable chemistry. In this study, the catalytic activity of rare-earth metal triflates in conjunction with choline chloride was reported, which can selectively convert glucose and fructose into hydroxymethylfurfural (HMF). The catalytic system showed good activity and selectivity, with yields of 94% and 99% for glucose and fructose, respectively, using 8% and 4% molar quantities of catalyst.
Article
Chemistry, Physical
Fabrizio Lo Celso, Giampaolo Barone, Loredana Maiuolo, Vincenzo Algieri, Carmen Cretu, Pietro Calandra
Summary: This study investigates the solubility and molecular dynamics of two newly synthesized oxindole nitrones with proven antiproliferative and antioxidant activity in liquid amphiphile solvents using a combination of experimental and computational methods. The different chemical structures of the solvents provide different chemical environments for the nitrones, and the study highlights the intermolecular interactions involved in the stabilization of nitrones in the solvents, providing valuable information for the design of specific carriers for nitrones delivery.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Physical
Chiara Abate, Donatella Aiello, Massimiliano Cordaro, Ottavia Giuffre, Anna Napoli, Claudia Foti
Summary: This study investigates the thermodynamic properties and chelating behavior of a naturally occurring dipeptide, L-carnosine, in its interactions with three transition metal cations, Cu2+, Mn2+, and Zn2+. Potentiometric and NMR titration experiments were conducted to determine the speciation models and formation constants of the metal complexes. The study also utilized High Resolution Matrix Assisted Laser Desorption Ionization Mass Spectrometry to further understand the chelating mode. The sequestering ability of carnosine towards Cu2+, Mn2+, and Zn2+ was quantified under physiological conditions.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biochemistry & Molecular Biology
Vincenzo Algieri, Paola Costanzo, Matteo Antonio Tallarida, Fabrizio Olivito, Antonio Jiritano, Giulia Fiorani, Francesca Peccati, Gonzalo Jimenez-Oses, Loredana Maiuolo, Antonio De Nino
Summary: This study reports the regioselective synthesis of a novel series of 1,5-disubstituted-1,2,3-triazole derivatives functionalized with pyrimidine nucleobases via 1,3-dipolar cycloaddition reaction. The synthesized compounds exhibited good pharmacokinetic properties and potential binding capabilities to viral and bacterial protein receptors based on molecular docking simulations.
Article
Pharmacology & Pharmacy
Cristina Algieri, Chiara Bernardini, Saverio Marchi, Maurizio Forte, Matteo Antonio Tallarida, Franca Bianchi, Debora La Mantia, Vincenzo Algieri, Rosita Stanzione, Maria Cotugno, Paola Costanzo, Fabiana Trombetti, Loredana Maiuolo, Monica Forni, Antonio De Nino, Flavio Di Nonno, Sebastiano Sciarretta, Massimo Volpe, Speranza Rubattu, Salvatore Nesci
Summary: Small molecules of triazole derivatives (Tzs) rescue compromised cardiovascular cells by preventing mitochondrial dysfunction. The protective effect of Tzs on mitochondria and cell metabolism was confirmed, leading to improved cell viability. These findings have important implications for the treatment of cardiovascular diseases.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Noemi Baldino, Ilaria Carnevale, Olga Mileti, Donatella Aiello, Francesca R. Lupi, Anna Napoli, Domenico Gabriele
Summary: Industrial hemp, with its rich protein and oil content, has gained attention as a new raw material in the food industry. A study on the extraction process of hemp oil using supercritical CO2 and ultrasounds revealed that it can be a high-quality alternative to traditional animal sources. The waste from extraction can be utilized in food formulations, and a stable emulsion can be formed by adding thickening polysaccharides. This research offers possibilities for innovative food products with high nutritional value.
APPLIED SCIENCES-BASEL
(2022)
Article
Pharmacology & Pharmacy
Vincenzo Algieri, Cristina Algieri, Paola Costanzo, Giulia Fiorani, Antonio Jiritano, Fabrizio Olivito, Matteo Antonio Tallarida, Fabiana Trombetti, Loredana Maiuolo, Antonio De Nino, Salvatore Nesci
Summary: An efficient, eco-compatible, and inexpensive method for constructing fully substituted pyrazoles (Pzs) was developed via the eliminative nitrilimine-alkene 1,3-dipolar cycloaddition (ENAC) reaction. The reaction yielded high regioselectivity and excellent yield, with enaminones and nitrilimines as dipolarophiles and dipoles, respectively. The F1FO-ATPase activity and mitochondrial permeability transition pore (mPTP) opening were evaluated for three different structures of Pzs, showing reduced activity and concentration-dependent inhibitory effects.
Article
Biochemistry & Molecular Biology
Luca Frattaruolo, Federica Marra, Graziantonio Lauria, Carlo Siciliano, Rosita Curcio, Luigina Muto, Matteo Brindisi, Donatella Aiello, Anna Napoli, Giuseppe Fiermonte, Anna Rita Cappello, Marco Fiorillo, Amer Ahmed, Vincenza Dolce
Summary: Dyslipidemia is a metabolic disorder associated with lipid imbalance, leading to conditions such as atherosclerosis and cardiovascular diseases. Statins are the main pharmacological therapy, but their limitations have prompted the search for new strategies. This study investigated the hypolipidemic effects of a picrocrocin-enriched fraction derived from saffron extract in HepG2 cells. The results suggest that this compound possesses interesting hypolipidemic effects through a non-statin-like mechanism, highlighting the biological potential of saffron as a useful adjuvant in balancing blood lipid homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Fabrizio Olivito, Vincenzo Algieri, Antonio Jiritano, Matteo Antonio Tallarida, Paola Costanzo, Loredana Maiuolo, Antonio De Nino
Summary: In this study, we assessed the potential of two synthesized bio-based polyurethane foams, PU1 and PU2, for the removal of diesel and gasoline from water mixtures. The results showed that both PU1 and PU2 had a higher sorption capacity for diesel/water mixtures compared to gasoline/water mixtures. The sorption process followed a pseudo second-order kinetic model, and the best isotherm models were Langmuir and Redlich-Peterson models. Furthermore, the foams exhibited even better performance in continuous-flow experiments, and can be regenerated up to 50 times without losing efficacy through centrifugation.
Article
Materials Science, Multidisciplinary
Giorgia Pagnotta, Maila Becconi, Marco Malferrari, Donatella Aiello, Anna Napoli, Luana Di Lisa, Stefano Grilli, Stefania Rapino, Maria Letizia Focarete
Summary: 3D bioprinting is a promising technology to replicate the complex characteristics of real tissues in vitro. Researchers developed a green synthetic route to create bioink formulations using HeLa cells, achieving good cellular viability and tunable mechanical properties. A systematic study was conducted to investigate the effects of the photoinitiator concentration, crosslinking process, and mechanical properties on cell viability.
MATERIALS ADVANCES
(2023)
